Identification of autotoxic compounds from Atractylodes macrocephala Koidz and preliminary investigations of their influences on immune system.

Structural characterization and evaluation the elicitors activity of polysaccharides from Chrysanthemum indicum

A fungal elicitor induces Sclerotium rolfsii sacc resistance in Atractylodis maceocephalae koidz.

OBJECTIVE To study on extraction of total polysaccharides in Chrysanthemi Indici Flos.And determine total polysaccharides in water extraction of Chrysanthemi Indici Flos from various habitats.METHODS Total polysaccharides were determined with phenol-sulfuric acid method and orthogonal test was used to select the best coloration.RESULTS Recovery of total polysaccharides was 100.3%,RSD was 1.19%,there was difference among Chrysanthemi Indici Flos from various habitats.CONCLUSION The method is accurate,repeatable and suitable to determine total polysaccharides in water extraction of Chrysanthemi Indici Flos.

Study on Extraction of Total Polysaccharides in Chrysanthemi Indici Flos

CIP elicitors on the defense response of A. macrocephala and its related gene expression analysis.

Atractylenolides compounds (atractylenolide-I, atractylenolide-II and atractylenolide-III) extracted from Atractylenolides macrocephala were analysed for their differential presence in the rhizome of both the infected and healthy plants and its fungitoxicity on prophylactic treatment with polysaccharides of Chrysanthemum indicum against Sclerotium rolfsii. It was evident that the plant under stress by pathogen has instigated the significant synthesis and accumulation of atractylenolide-II and atractylenolide-III in the rhizome of plants as a result of elicitation with polysaccharides of C. indicum. Our results also showed that the absence of atractylenolide-II in the rhizome made the plant susceptible to S. rolfsii. Furthermore, on in vitro antifungal studies, exogenous application of atractylenolide-II with the concentration of 200 μg/ml showed a significant inhibitory effect on mycelial growth by achieving a 77.23% antifungal activity rate, and a minimal inhibitory effect at a concentration of 12.5 μg/ml with atractylenolide-II. To our knowledge, this novel study on phytoalexins in A. macrocephala suggested that A. macrocephala plants produce elevated levels of atractylenolide-II and atractylenolide-III in a pattern typical of phytoalexins, in response to an eliciting treatment after infection.

Evidence of Phytoalexins in Rhizome of Atractylodis Maceocephalae Koidz Infected with Sclerotium rolfsii sacc Following Treatment with the Polysaccharides of Chrysanthemum indicum

Yinfeng Zhou

Papers

Evidence of Phytoalexins in Rhizome of Atractylodis Maceocephalae Koidz Infected with Sclerotium rolfsii sacc Following Treatment with the Polysaccharides of Chrysanthemum indicum