Biotransformation studies using hairy root cultures — A review

Biotransformation Through Hairy Roots: Perspectives, Outcomes, and Major Challenges

Objective: To investigate the biotransformation of eight active constituents chemicals by hairy roots of Polygonum multiflorum Thunb. Methods: Active compounds were added to the culture system of hairy roots of P.multiflorum after precultured for 9 d, then they were co-cultured for another 7 d. The products were isolated and purified by column chromatography. Results: Three of the eight active constituents were identified to be biotransformed, which were furannoligularenone (6), 1,4-benzenediol (7) and artemisinin (8). HPLC analysis of the biotransformation of 6 showed two new peaks except the peak of substrate, then the corresponding two compounds were obtained; Substrate 7 was converted into arbutin. In the case of 8, there were two new peaks and the corresponding two monomers have been also isolated. Conclusion: The result showed that there were several enzymes in the culture system of P.multiflorum hairy roots, which could processed glycosylation, oxidation, reduction and hydroxylation reactions.

YU Rong-min

Papers

Biotransformation of Eight Active Constituents by Hairy Roots of Polygonum multiflorum Thunb