The invention discloses a method for breeding purple-black special rice. The method comprises the steps of selecting purple-black leaf rice as parent A, selecting rice with purple-black chaffs and seed coats as parent B, and mating parent A with parent B to obtain F1 seeds; planting single cross F1 seeds and backcrossing with the parent A to obtain BC1F1 seeds; planting BC1F1 seeds; using anther culture to construct a double haploid population and harvesting seeds according to per plant; planting DH1 seeds, and selecting strains with purple-black leaves, chaffs and seed coats; planting DH2 toDH3 seeds, selecting good rice varieties with a japonica rice and rice blast comprehensive resistance index of less than grade 9; planting DH4 seeds, selecting case-hardened rice with purple-black leaves, chaffs and seed coats and a rice blast comprehensive resistance index of less than grade 9, and obtaining the purple-black special rice. The new rice variety selected by the invention has purple-black leaves and chaffs, and is more ornamental. Combined with high value-added and deep-processing applications of purple-black special rice, the new rice variety is beneficial to combination of agriculture and tourism.

Method for breeding purple-black special rice

The invention discloses a breeding method for red endosperm rice. The method comprises the following steps: using rice containing starch-fat complexes as a parent A, using rice with a red seed coat asa parent B, and using the parent B as a female parent, hybridizing with the parent A, to obtain a F1-generation; backcrossing with the parent A, to obtain a BC1F1-generation; selfing, to obtain BC1F2-generation seeds, selecting and remaining grains with the red seed coat, constructing a double haploid; and successively planting DH1-DH3 generations and selfing, using a pale yellow seed coat, red endosperm, crude fat content greater than or equal to 2% and more than 70% of the starch-fat complexes as screening indexes, to harvest the red endosperm rice. The rice obtained by breeding has the advantages of pale yellow seed coat, red endosperm, high starch-fat complex content and high anthocyanin content, and anthocyanin is effectively expressed in a part of the edible endosperm, a natural color product, such as red rice noodles, can be developed without using a food colorant, and the rice is particularly in comply with general requirements of people to natural and healthy food.

Breeding method for red endosperm rice

The invention provides a method for culturing transgenic rice GRH and black gold rice and detecting a target gene of the transgenic rice GRH, and belongs to the field of seed breeding of rice. The method for culturing the transgenic rice GRH comprises the following steps of (1) building a final expression vector; (2) transforming the heredity ofperforming genetic transformation on the final expression vector, so as to obtain a T0-generation transformed plants; (3) screening the plants with positive target plants in the T0-generation transformed plants, and selfing to fruit, so as to obtain a T1-generation transformed plants; (4) screening the plants without hpt expression in the T1-generation transformed plants, so as to obtain the transgenic rice GRH. The transgenic rice GRH and the blackrice are used as the parents to culture the black gold rice which is rich in anthocyanin, lutein and beta-carotenoid, so as to obtain the certain eye-protection and health-care effects. The method has the advantages that the method is simple and reliable, the transformation rate is high, the deactivating of the endogenous gene of the rice due to insertion of the target genes is avoided, and the good commercializing potential is realized.

Method for culturing transgenic rice GRH and black gold rice and detecting target gene of the transgenic rice GRH

The invention relates to an efficient creation method of nonglutinous rice with high flower culture induction rate. The efficient creation method comprises the following steps: firstly hybridizing a parent of which the flower culture induction efficiency is more than 50 percent with multiple breeding backbone parents to obtain hybrid F1 seeds of different combinations; and then planting the hybridF1 seeds, taking young ears for anther culture for the first time, screening out the combinations of which the respective induction efficiency is more than 60 percent and harvesting anther culture H1seeds by single plants; then planting H1 seeds by strains, taking young ears for anther culture for the second time and screening out the strains of which the respective induction efficiency is morethan 60 percent. According to the efficient creation method provided by the invention, not only can the stable strains with high flower culture induction rate be obtained, but also the period is shorter.

Efficient creation method of nonglutinous rice with high flower culture induction rate

The invention discloses a rice breeding method, comprising the following steps: performing hybridization by taking a black rice giant embryo rice variety as a male parent and a high-zinc variety as a female parent to obtain F1 generation seeds; backcrossing F1 plants with the black rice giant embryo rice variety once to obtain BC1F1 seeds; planting BC1F1 plants, constructing a doubled haploid population by adopting an anther culture technology, and harvesting DH0 seeds according to individual plants; for a DH1 line, selecting individual plants with aggregation of three nutrient characteristics namely black rice, giant embryo and high zinc content; for a DH2 line, selecting strains with good rice blast resistance; for a DH3 line, selecting strains with a high yield; and for a DH4 line, breeding a constant strain, thereby obtaining a high-zinc giant embryo black rice variety. The hybrid rice provided by the invention aggregates the three good characteristics namely black rice, giant embryo and high zinc content, the contents of certain microelements are improved, and a technology of performing backcrossing with a parent and then performing anther culture is adopted, so that good comprehensive characteristics of the parent are better preserved, the properties are stabilized quickly and can be evaluated early, and the breeding period is short.

Zhang Ning

Papers

Rice breeding method