The paper presents qualitative and quantitative composition of the nucleotide sequences of the 5'‑region of the first subunit of the mitochondrial cytochrome oxidase (COI) gene (decoded in the framework of the International DNA Barcoding Project) and the variability of the corresponding amino acid sequences in the genus Jordanita Verity, 1946. The number of variable positions in the COI region (219 amino acid residues) is 14.2% that significantly exceeds the data for other Lepidoptera. Thirty one amino acid variable positions were revealed, and two of them are localized near the active site of the enzyme.

Molecular Genetic Characters of Species of the Genus Jordanita Verity, 1946 (Lepidoptera: Zygaenidae, Procridinae): DNA Barcodes and Corresponding Amino Acid Sequences

Four new species of the genus Phantasca Redtenbacher, 1906 (Phantasca bulbosa n. sp., P. arlequina n. sp., P. kawensis n. sp. and P. margaritae n. sp.) from French Guiana are described and illustrated. The female of P. femorata Hennemann, Conle, Bellanger, Lelong & Jourdan, 2018, is re-described due to subsequent discovery of an adult specimen, and the previously unknown egg is described for the first time. The male of P. ruboligata Hennemann et al., 2018, is re-described with the holotype illustrated to rule out confusion with the male of P. margaritae n. sp. A barcoding study based on COI gene of eight species of Phantasca is presented and supports their inclusion within the genus.

Supplements to the knowledge of Phantasca Redtenbacher, 1906, with the descriptions of four new species from French Guiana (Phasmatodea, Diapheromeridae, Diapheromerinae)

The aim of this article is to contribute to the recent distribution and systematic classification of Zygaenidae (Lepidoptera) in 12 provinces in the Aegean, eastern Anatolia and Thrace regions of Turkey, with the records obtained in 2020 and 2021. Twenty Zygaenidae species from five genera belonging to Procridinae and Zygaeninae subfamilies were recorded: Theresimima Strand, 1917 (1 species), Rhagades Wallengren, 1863 (1 species), Adscita Retzius, 1783 (1 species), Jordanita Verity, 1946 (5 species), and Zygaena Fabricius, 1775 (12 species). It is the first record of Zygaena (Zygaena) ephialtes (Linnaeus, 1767) in Thrace region of Turkey since Herrich-Schäffer (1845). Zygaena (Mesembrynus) lydia Staudinger, 1887 endemic to Turkey, was also recently recorded in this study. In addition, Zygaena (Agrumenia) armena Eversman, 1851, which is known only in Turkey and Georgia, was also recorded from the province of Ardahan in the eastern Anatolia region of Turkey.

Contribution to the knowledge of the zygaenidae (lepidoptera) of turkey

Son yıllarda pek çok böcek grubuyla birlikte Lepidoptera takımında da türlerin teşhis edilmesinde moleküler çalışmalar artarak önem kazanmış ve karşılaştırmalı morfolojik sınıflandırmanın tamamlayıcısı olmuştur. Tüm dünyaya yayılmış ve kendi içinde beş alt familyaya bölünmüş Zygaenidae familyasının ülkemizde 54 türü bulunmaktadır. Bu çalışmada Doğu Akdeniz Bölgesi’ndeki 31 lokasyondan toplanan 13 Zygaenidae türü kullanılarak DNA izolasyonu ve PCR analizlerinde optimizasyon çalışmaları yapılmıştır. DNA izolasyonu aşamasında “Qiagen DNeasy DNA izolasyon kiti” (QDNA) ve “Macherey Nagel Nucleospin DNA Insect kiti” (MN) kullanılmıştır. Zygaenidae familyası ile ilgili yapılacak çalışmalarda MN kitinin, QDNA kitinden daha etkili ve başarılı sonuç verdiği belirlenmiştir. Ayrıca çalışmada nanodrop ölçüm sonuçlarının yol gösterici olmadığı görülmüştür. Gelecekte yapılacak çalışmalarda ise daha farklı DNA ölçüm tekniklerinin denenmesinin faydalı olacağı düşünülmektedir. PCR analizlerinde ise iki farklı karışım, DreamTaq PCR karışımı ile primer ve laboratuvar koşullarına göre modifiye edilmiş PCR karışımı, denenmiştir. Ticari karışımın çok daha hızlı ve etkin sonuç verdiği ancak olanaklar doğrultusunda ihtiyaç duyulması halinde ise laboratuvarda oluşturulan karışımla da başarılı sonuçlar elde edilebileceği görülmüştür. Çalışmada kullanılan LCO1490/HCO2198 primer çiftinin Zygaenidae türlerinin tanılanması için uygun olduğu saptanmış ve analizlerde tür tanılamasında %92.7 oranında başarı elde edilmiştir 

/pdf/zygaenidae-lepidoptera-familyasinda-dna-izolasyonu-ve-pcr-275zer3q.pdf

Zygaenidae (Lepidoptera) familyasında DNA izolasyonu ve PCR optimizasyonu üzerine araştırmalar

Harsh environments (e.g., hypoxia and cold temperatures) of the Qinghai–Tibetan Plateau have a substantial influence on adaptive evolution in various species. Some species in Lycaenidae, a large and widely distributed family of butterflies, are adapted to the Qinghai–Tibetan Plateau. Here, we sequenced four mitogenomes of two lycaenid species in the Qinghai–Tibetan Plateau and performed a detailed comparative mitogenomic analysis including nine other lycaenid mitogenomes (nine species) to explore the molecular basis of high-altitude adaptation. Based on mitogenomic data, Bayesian inference, and maximum likelihood methods, we recovered a lycaenid phylogeny of [Curetinae + (Aphnaeinae + (Lycaeninae + (Theclinae + Polyommatinae)))]. The gene content, gene arrangement, base composition, codon usage, and transfer RNA genes (sequence and structure) were highly conserved within Lycaenidae. TrnS1 not only lacked the dihydrouridine arm but also showed anticodon and copy number diversity. The ratios of non-synonymous substitutions to synonymous substitutions of 13 protein-coding genes (PCGs) were less than 1.0, indicating that all PCGs evolved under purifying selection. However, signals of positive selection were detected in cox1 in the two Qinghai–Tibetan Plateau lycaenid species, indicating that this gene may be associated with high-altitude adaptation. Three large non-coding regions, i.e., rrnS-trnM (control region), trnQ-nad2, and trnS2-nad1, were found in the mitogenomes of all lycaenid species. Conserved motifs in three non-coding regions (trnE-trnF, trnS1-trnE, and trnP-nad6) and long sequences in two non-coding regions (nad6-cob and cob-trnS2) were detected in the Qinghai-Tibetan Plateau lycaenid species, suggesting that these non-coding regions were involved in high-altitude adaptation. In addition to the characterization of Lycaenidae mitogenomes, this study highlights the importance of both PCGs and non-coding regions in high-altitude adaptation.

https://www.frontiersin.org/articles/10.3389/fgene.2023.1137588/pdf

Comparative mitogenomic and evolutionary analysis of Lycaenidae (Insecta: Lepidoptera): Potential association with high-altitude adaptation

https://nl.pensoft.net/article/33190/download/pdf/

Zoya S. Lazareva

Papers

DNA barcoding of Zygaenidae (Lepidoptera): results and perspectives