Showing papers by "Bial published in 1979"
Bial1
TL;DR: The AFP from human cord blood was isolated by means of affinity chromatography with the use of antibodies as ligands and by gel filtration and homogeneity of the purified AFP was demonstrated.
Abstract: The AFP from human cord blood was isolated by means of affinity chromatography with the use of antibodies as ligands and by gel filtration. The preliminary purification was achieved by affinity chromatography on CNBr-Sepharose 4B coupled with anti AFP-antibody. Further purification was obtained by the use of immunoadsorbent with anti-human serum protein antibodies. Final purification was achieved by gel filtration on Sephadex G-200. Homogeneity of the purified AFP was demonstrated by means of gel filtration, polyacrylamide gel electrophoresis, isoelectric focusing and immunoelectrophoresis.
6 citations
Bial1
TL;DR: It is suggested that AFP plays an important role in immunoregulation during the fetal life and inhibits both blastic transformation and immunoglobulin release in lymphocyte cultures.
5 citations