Showing papers by "Kagawa University published in 1976"
TL;DR: A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen and produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium.
Abstract: A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C.
19 citations
TL;DR: It was suggested that protein synthesis causally linked with resistance expression of Shokan 1 leaves to race 226 occurred between 14 and 24hr after inoculation, and blasticidin S markedly inhibited the incorporation of 14C-leucine into acid insoluble fraction.
Abstract: Blasticidin S completely supressed the resistance expression of Shokan 1 oat leaves to Puccinia coronata avenae race 226 when 5×10-6M aqueous solution of the chemical was supplied to the plants for 4hr from stem cut-ends from 8 or 12hr after inoculation. This blasticidin S effect was decreased as the initiation of the chemical supply was delayed to 14 or more hr after inoculation, and no effect was found by the supply after 24hr. The ratio of 14C-leucine incorporation into acid insoluble fraction to total uptake increased in the resistant-responding leaves to 1.2- to 1.35-fold of non-inoculated control, when the leaves were fed with 14C-leucine for 1 and 4hr from 14 to 24hr and 14 to 18hr after inoculation, respectively. No obvious increase in the ratio of incorporation was observed during the period of experiments in the leaves which were inoculated with compatible race 203. Blasticidin S markedly inhibited the incorporation of 14C-leucine into acid insoluble fraction in all cases including the blasticidin S treatment was initiated at 24hr after inoculation, in which no effect of blasticidin S on the resistance was detected. It was suggested that protein synthesis causally linked with resistance expression of Shokan 1 leaves to race 226 occurred between 14 and 24hr after inoculation. Peroxidase isozymes and phenylalanine ammonia-lyase activity increased in leaves inoculated with race 226, but the increases were not reduced by the blasticidin S treatment. It was assumed, therefore, that the resistance in this host-pathogen system involved the synthesis of protein(s) other than these enzymes.
18 citations
16 citations
14 citations
TL;DR: The strain of E. coli in the title was found to produce considerably this enzyme adaptively, and showed optimum pH and temperature at 6.8 and 37°C, respectively, with the substrate p-nitrophenyl-α-D-galactoside (PNPG).
Abstract: Galactosylsucroses contained in soybeans are not digestible. Thus we wished to detect α-galactosidase (EC 3. 2. 1. 22) in intestinal bacteria. The strain of E. coli in the title was found to produce considerably this enzyme adaptively. We could prepare rather pure solution of the enzyme from the sonicate of the strain. It was purified about 142-fold. It showed optimum pH and temperature at 6.8 and 37°C, respectively, with the substrate p-nitrophenyl-α-D-galactoside (PNPG). Dilute enzyme solutions were very unstable even at 0_??_5°C. However, concentrated solutions were considerably stable. The Michaelis constant (M) was 1.07×10-4, 2.33×10-3, and 3.65×10-1 for PNPG, melibiose, and raffinose, respectively, The maximum velocity (mole/min/mg protein) was 2.72×10-5, 2.67×10-5, and 2.04×10-5, respectively for the same three substrates. This enzyme had a weak transferase action.
12 citations
11 citations
TL;DR: Observations strongly indicate that production and excretion of ED are characteristic of Shokan 1 responding hypersensitively to race 226, though causal linkage of these events with the resistance expression has not yet been made clear.
Abstract: Primary leaves of 7-day-old seedlings of three oat cultivars were inoculated with uredospores of two races of Puccinia coronata avenae and were subjected to electron microscopic observations in order to elucidate changes in fine structure during the resistance expression. Shokan 1 responds to race 226 with hypersensitive necrosis and fungal growth ceases 35-40hr after inoculation. Cellular disorganization in mesophyll tissue was found by 28hr after inoculation, subsequent to the invasion of the first haustoria. No decompartmentalization of subcellular organelles could be detected in host cells around the infected sites at least within 20hr after inoculation. This fact supports our previous proposal that in this host-pathogen system the determination of resistance which occurs between 8 and 12hr after inoculation is not triggered by the collapse of host cells. During the prehaustorial stage of infection of Shokan 1 with race 226, the number of Golgi apparatus increased, comparing with that of non-inoculated control, and Golgi vesicles containing an electron dense material (ED), which had never been observed in healthy leaves, became abundant. ED was also observed as a deposite at intercellular space of mesophyll tissues and on inner surface of host cell walls where the host plasma membrane invaginated. Large ED particles free from enclosures were occasionaly observed in the host cytoplasm. Contrary to Shokan 1, in Hyuga-Kairyo-Kuro, resistant to race 226, the fungus continues to grow till 72-96hr after inoculation and no necrotic symptom appears. In this cultivar, the number of Golgi apparatus in mesophyll cells increased after infection, but ED was seldom observed in Golgi vesicles and no deposition of ED occurred. Neither production of ED-containing vesicles nor deposition of ED was observed in leaves of compatible combination, i.e. Shokan 1 and Hyuga-Kairyo-Kuro inoculated with race 203, and Victoria 226-S inoculated with race 226. These observations strongly indicate that production and excretion of ED are characteristic of Shokan 1 responding hypersensitively to race 226, though causal linkage of these events with the resistance expression has not yet been made clear.
5 citations
3 citations
TL;DR: In this paper, an acid-and heat-stable β-Fructofuranosidase from Corticium rolfsii was presented. But the β-fructofuranase was not shown to work well in the presence of acid and heat.
Abstract: (1976). An Acid- and Heat-stable β-Fructofuranosidase from Corticium rolfsii. Agricultural and Biological Chemistry: Vol. 40, No. 10, pp. 2107-2108.
2 citations
TL;DR: A new step of NAD metabolism was shown in Aspergillus niger and a part of the radioactive precursors was also incorporated into an unknown compound that was proved to be a terminal metabolite in the NAD pathway.
Abstract: A new step of NAD metabolism was shown in Aspergillus niger. Radioactive nicotinic acid and nicotinamide were incorporated into nicotinamide ribose diphosphate ribose (NAm-RDPR), which had been isolated from the culture filtrate. Its content in the culture medium increased with an increase of culture time, and this compound was proved to be a terminal metabolite in the NAD pathway. The experimental results also showed that the Preiss-Handler pathway and the NAD cycling system function in the NAD biosynthesis in A. niger. A part of the radioactive precursors was also incorporated into an unknown compound.
1 citations
TL;DR: In this article, partial wave amplitude of K+p elastic scattering is investigated from the information on the inelastic channels, Kil (1236) and K* (891) p in terms of the three channel N/D model.
Abstract: P" partial wave amplitude of K+p elastic scattering is investigated from the information on the inelastic channels, Kil (1236) and K* (891) p in terms of the three channel N/ D model. The calculated P" phase shift shows a counterclockwise circle in the Argand diagram and has the maximum of phase shift versus energy at 1.15 Ge V / c of kaon incident momentum. \Ve argue that the exotic resonance Z,*, if it exists, is a highly inelastic resonance caused by the strong absorption through inelastic channels and is coupled very weakly with K+p elastic channel.