Showing papers by "National Chung Hsing University published in 1977"
TL;DR: Two methods of in vitro culture were employed to regenerate papaya plants, one involved regeneration of plants from callus and the other, production of multiple plants from single shoot-tip explants.
Abstract: Two methods of in vitro culture were employed to regenerate papaya plants. One involved regeneration of plants from callus and the other, production of multiple plants from single shoot-tip explants. Callus was induced from stem sections of papaya seedlings in a medium containing 1 mg per 1 NAA and 0.1 mg per 1 kinetin. The callus regenerated shoots and/or embryoids when transferred to a medium of lower auxin, 0 to 0.05 mg per 1 IAA, and higher cytokinin, 1 to 2 mg per 1 kinetin. Multiple shoots were produced when the excised shoot-tip explants were cultured in a medium supplemented with 0.05 mg per 1 IAA and either 5 mg per 1 kinetin or 0.5 to 1.0 mg per 1 benzyladenine. Root formation of the shoots or embryoids that derived from callus or shoot tips occurred in a medium containing 5 mg per 1 IAA and in a light intensity of 3000 to 4000 lx. The rooted plants could be established in soil and under standard greenhouse conditions after they had been acclimated by initially growing them in moist vermiculite contained in polyethylene-covered pots.
75 citations
TL;DR: In this article, the authors performed a study on twenty two surface soils, representing six parent materials and four soil groups, were sampled in Taiwan for the present study and the results indicated that the ammonium oxalate-extractable Al and Fe are related to the contents of organic matter but not to acidity and the contents in clay.
8 citations