Showing papers in "European Journal of Cell Biology in 1980"

TL;DR: In ovulated mouse oocytes, IPC disturbed microtubule orientation and the relative distance between MTOCs and chromosomes, however this herbicide did not seem to act on polymerized microtubules and its action was apparently reversible.

TL;DR: This review provides an integrated description of normal meiosis and ascospore genesis for all eight-spored Neurosporas with photographs of asci stained with iron-hematoxylin and maturing asci from crosses with selected mutants and a chromosome rearrangement are illustrated.

TL;DR: The data show that aromatic hydrocarbons are metabolized not only in ER membranes but also in other kinds of membranes, including Golgi apparatus and plasma membrane, and the mode and extent of this metabolism can differ in different membranes.

TL;DR: In the hamster small intestine, membrane-bound bodies are formed individually and in groups from the plasma membrane surrounding the microvilli in cells of the small intestine as mentioned in this paper, and they retain at least a portion of glycocalyx.

TL;DR: Rat peritoneal mast cells were separated by using density gradients of PVP-coated silica particles (Percoll) and Percoll was found to be superior to earlier isolation procedures by giving a practically pure and intact mast cell suspension and by avoiding cell aggregation.

TL;DR: It is hypothesize that the two proteins do not travel in-parallel towards the Golgi complex, and that, compared with Chtg, Am would lag behind in the RER cisternae.

TL;DR: In this article, structural models for the organization of the peripheral nucleolar strands, the Balbiani ring (BR) transcription axes, and the tubular rough endoplasmic reticulum (RER) were formulated.

TL;DR: In this work the structure of spontaneous nucleolar vacuoles is compared with that induced by drugs such as cordycepin, and FUdR and corresponds to a transient structure which not only shows higher metabolic activity but also supplies a storing and/or transporting mechanism for nucleolar products.

TL;DR: Mouse peritoneal macrophages were cultured in vitro, exposed to exogenous tracers, and examined by transmission electron microscopy to confirm previous notions concerning a role of cytoplasmic microtubules in endocytosis and indicate that absence ofmicrotubules affects the intracellular handling of molecules binding to the cell surface.

TL;DR: It is concluded that these microfilaments may constitute one structural component of the traction apparatus that moves chromosomes during metakinesis and anaphase, and that at least some (and possibly many) of the MTs of the kinetochore fiber are derived from those entering the nucleus at prometaphase.

TL;DR: Evidence is considered for the hypothesis that cellular retraction, the apparent disorganization of stress fibers, and the redistribution of contractile proteins result from unremittent energy-dependent contraction induced by CD, and that the compact forms may be analogous to rigor complexes.

TL;DR: Root primordia of the water fern Azolla pinnata were examined by conventional and high voltage electron microscopy to extend previous evidence concerning the existence and behaviour of nucleating sites (NS) for microtubule arrays in the cortex of plant cells.

TL;DR: Three aspects of the location and properties of pre-prophase bands of microtubules inplant tissues were examined, with particular reference to possible roles of the pre-Prophase band site after its microtubule have disappeared at prophase.

TL;DR: Mitosis and cytokinesis have been followed in live cells using Nomarski and birefringence optics, with emphasis on the dynamics and mechanics of spindle assembly, elongation and disassembly.

TL;DR: A 30 to 50% inhibition of photosynthesis and respiration as well as calcium uptake was caused by the herbicides, whereas colchicine did not affect these metabolic processes.

TL;DR: Inside and around the mitotic apparatus a vesicular, a tubular and a cisternal type of membranes are demonstrated and elements of the tubular ER are often aggregated in parallel arrays at the periphery of the spindle region, thus resembling dictyosomes.

TL;DR: Immunofluorescence cytochemistry showed that GP-2 antigens were exclusively located in the exocrine pancreas, where they were preferentially found in the perimeter of secretory granules and in the acinar lumina, suggesting the possibility of loss of GP -2 from exocrine cells during secretion and raising doubts as to the status of GP-1 as a true membrane glycoprotein.

TL;DR: The results indicate that phalloid-in-stabilized filamentous actin can still give rise to contraction within the stress fiber system, and that F-actin bound to phaloidin may be translocated within the cell.

TL;DR: It would appear that the endomembrane concept sensu strictu is not applicable to higher plant cells whose main secretory product is polysaccharide rather than protein, and consequences and alternatives in relation to membrane-flow in such cells are discussed.

TL;DR: The data are interpreted to indicate that the predominant, if not exclusive form of supranucleosomal packing existing in condensed inactive chromatin of living cells is a chromatin fibril made up of serially arranged, close-packed granular particles ("suprabeads" sensu in [64]).

TL;DR: The unravelling of the large supranucleosomal granule into the extended nucleofilament chain in low salt buffers seemed to be a gradual process since intermediate stages of variable particles sizes and shapes were observed.The organization of the chromatin of sperm nuclei of sea urchin was studied by electron microscopy using thin sections of fixed cells and spread preparations of chromatin obtained after various procedures involving incubation inLow salt buffers.

TL;DR: Pairs of fluorescent A-T specific dyes and nonfluorescent agents with similar or complementary base pair binding specificity were used to analyse the extent to which banding patterns in human chromosomes obtained by fluorescent staining can be modified by counterstaining.

TL;DR: In cells of Rhodopseudomonas capsulata, grown at growth limiting light intensity, the cellular bacteriochlorophyll content increased 13-fold, the Bchl concentration of membranes 3.4 fold, and the number of intracytoplasmic membrane vesicles, bearing the photosynthetic apparatus, 6..3 fold in comparison with cells grown at 2000 W x m-2 light intensity.

TL;DR: The highly helical soluble proteolytic fragments of mature alpha-keratin contains antigenic determinants shared by the cytokeratins present in non-epidermal cells, and antibodies to these keratin fragments can be used for the demonstration of at least some cytokeratin-containing structures in other cells and tissues.

TL;DR: In sedimentation analyses of partially dissociated phycobilisomes a fragment consisting of two phycoerythrin hexamers associated with one polar C-phycocyanin hexamer with a sedimentation constant of 22 S were demonstrated and the corresponding molecular weight of the tripartite units coincides well with morphological measurements on the basis of an average protein packing density and with earlier estimates on cross-linked biliprotein aggregates in gradient gel electrophoresis.

TL;DR: The results show that micro Tubules can undergo a disruption-reorganization process without changes in the organization of intermediate filaments and suggest that the maintenance of normal intermediate filament organization is independent of microtubules.

TL;DR: The fractional rate of degradation was constant over a twelve-fold range of intracellular asialo-fetuin concentrations and the average activation energies of degradation over the range 20 to 40 degrees C were 125 kJ/mol for asialosomucoid and 149 kj/mol; however, the Arrhenius plots were not straight lines over this temperature range.

TL;DR: The data support the concept of an altered nucleohistone conformation not only in transcribed chromatin but also in the vicinity of transcriptional events.