Showing papers in "Indian Journal of Medical Research in 1972"

Antifertility activity of an indigenous plant preparation (ROC-101). I. Effect on reproduction.

Abstract: Rats mice rabbits and guinea pigs were fed diets containing ROC-101 an herbal preparation from 3 plants which has been reported to prevent conception. Spayed female mice showed no reaction to the ROC-101 when administered. When immature mice were fed 10% RC-101 the establishment of vaginal patency was unaffected. When 10% was fed to adult mice the estrus cycle was interrupted. Mated females did not become pregnant. When both male and female mice had been fed RC-101 there was 100% inhibition of mating. At the 5% and 10% feeding level of females there was 100% inhibition of the females. Males fed at the lowest dose showed impaired fertility. The breeding performance of rats was affected by the fertility of adult female guinea pigs and rabbits was uneffected. ROC-101 does not appear to be 100% anti-ovuletory since newly formed corporalutea were seen in all breeded animals ovaries.

Classification of subjects as slow or rapid inactivators of isoniazid, based on the ratio of the urinary excretion of acetylisoniazid to isoniazid.

Abstract: Following an intramuscular injection of isoniazid 3 mg/kg body-weight, the urinary excretion of isoniazid and acetylisoniazid during the periods 0-1, 1-2, 2-3 and 3-4h was determined for 124 patients with pulmonary tuberculosis. On the same occasions, the serum isoniazid concentration at 4 ½h was determined by microbiologic assay. The ratios of acetylisoniazid to isoniazid in the urine collections at 2, 3 and 4 h were bimodally distributed. Rules were derived from these ratios for classifying subjects as slow or rapid inactivators of isoniazid. There was 100% agreement between the classification based on each of these ratios and that based on the serum isoniazid concentration at 4 ½h.

Antifertility activity of an indigenous plant preparation (ROC-101). II. Effect on the male reproductive system.

Abstract: ROC-101 a plant preparation was administered orally to male and female rats and mice to determine its antifertility effects. The antifertility effects were considerably greater in the male than in the female. 50 and 100 g/kg diet of ROC-101 inhibited spermatogenesis in mice. Over a 3-week period of treatment with the 100 mg dose the number of primary and secondary spermatocytes diminished considerably. Testicular weights were reduced by 75% compared to controls in animals receiving ROC-101 for 4 weeks. The testes in this group exhibited only Sertoli cells type A spermatogonia and primary spermatocytes. There was no evidence of androgenic or antiandrogenic activity when ROC-101 was tested in castrated immature mice and rats. The action of ROC-101 appears to be similar to that of ORF-1616.

Post-irradiation vascular injury and accelerated development of experimental atherosclerosis.

Abstract: An experiment was conducted in albino rabbits to elucidate the effect of focal x irradiation on the aorta and heart in the initiation as well as aggravation of atherosclerotic lesions. Two groups were fed cholesterol in olive oil for a period of 31 days and one of these groups was also given focal x irradiation in the beginning of the experiment. It was observed at the end of the experiment that the frequency incidence, extent, intensdty, and morphological complications were significantly greater in animals subjected to x irradiation and cholesterol feeding as compared to those given cholesterol alone. The determining factor for this change between the two groups appeared to be focal vascular trauma produced by x irradiation. (auth)