Journal of Chinese Practical Diagnosis and Therapy 

About: Journal of Chinese Practical Diagnosis and Therapy is an academic journal. The journal publishes majorly in the area(s): Gene polymorphism & Genotype. Over the lifetime, 99 publications have been published receiving 933 citations.

Intensive insulin therapy for the critically ill patients with stress hyperglycemia

Abstract: Objective To observe the effect of intensive insulin therapy on the critically ill patients with stress hyperglycemia in ICU.Methods One hundred and ten critically ill patients in ICU were randomly divided into two groups,the intensive insulin therapy group(n=55) and control group(n=55).The blood glucose in the intensive insulin therapy group was controlled at 4.4 to 6.1 mmol/L,and the blood glucose in control group was controlled at 10.0 to 11.1 mmol/L.The two groups were observed and compared the days in the ICU,the numbers of patients requiring mechanical ventilation,the days of mechanical ventilation,the incidences of infection in hospital,the days of using antibiotics,Acute Physiology and Chronic Health Evaluation Ⅱ score of the last day in ICU,the morbidity of multiple organ failure,the morbidity of hypoglycemia and mortality.Results All the above indices except morbidity of hypoglycemia were significantly lower in the intensive insulin therapy group than those in control group(P0.05 or P0.01).Conclusion Intensive insulin therapy and keeping blood glucose at 4.4 to 6.1 mmol/L can improve the clinical curative effect and reduce the mortality for the critically ill patients with stress hyperglycemia,

Umbilical cord mesenchymal stem cell transplantation for spinal cord injury

Abstract: Objective To explore the safety and efficacy of umbilical cord mesenchymal stem cell(UC-MSC)transplantation in the treatment of spinal cord injury.Methods Fifteen patients with spinal cord injury were treated with CT-guided UC-MSC transplantation(observation group).Another 15 patients received rehabilitation excise(control group).ASIA motor score,score of pain sensation,light touch sensation,ASIA scoring and the improvement rate of AIS grading were compared before and 6months after operation between two groups.Results The improvement rate of AIS grading was significantly higher in observation group(66.7%)than that in control group(13.3%)(P0.05).The motor score(33.24±19.45),score of pain sensation(49.45±19.67),light touch sensation(51.34±18.67)and ASIA scoring(134.03±57.79)in observation group 6 months after operation were significantly higher than those before operation(25.35±18.32,33.24±22.38,36.11±20.05,94.7±60.75),and higher than those in control group(27.89±21.07,37.83±22.87,39.66±24.83,105.38±68.77)(P0.05).There were no significant differences6 months after operation in comparison with those before operation(P0.05).No major adverse reactions occurred during operation and in follow-up survey in observation group.Conclusion UC-MSC transplantation can effectively improve neurological function and life quality of patients with spinal cord injury,and it is safe.

Influence of smoking and the polymorphisms of CYP1A1 and GSTM1 on the susceptibility of lung cancer

Abstract: Objective To study the relationship of cytochrome P-450 1A1(CYP1A1) and glutathione S-transferase M1 (GSTM1) polymorphisms with occurrence of lung cancer.Methods PCR-RFLP was used to determine genetic polymorphisms of CYP1A1 and GSTM1 in 103 patients with lung cancer and 138 healthy controls.Logistic regression analysis was used to assess the value of odds ratio (OR) and confidential intervals (CI) between different genotypes and smoking after adjusted by ages and gender.Results The allele frequencies of CYP1A1 M1 were 27.6% and 42.7% in control and lung cancer groups,respectively.The allele frequencies of GSTM1 deletion were 44.2% and 61.2% in control and lung cancer groups,respectively.OR for the CYP1A1 (B) or (C) genotype indicated 3.19 and 2.61-fold increased risk for developing lung cancer.The adjusted OR of the GSTM1 deletion genotype showed a 2.09-fold increased risk for lung cancer.Both associations were statistically significant.The increased risk for developing lung cancer was 5.72 folds for the individuals with combination of the CYP1A1(B or C) and GSTM1 deletion genotypes.CYP1A1 M1 and the GSTM1 deletion genotypes increased risk for developing small cell carcinoma and squamous carcinoma,significantly.Conclusion The mutant genotypes of the CYP1A1 M1 and GSTM1 deletion polymorphisms are the risk for lung cancer.CYP1A1 M1 and GSTM1 interact obviously,both of which have the joint effect with smoking.

Tumor lysis syndrome in patients with acute myeloid leukemia

Abstract: Objective To analyze the incidence,biochemical features,high risk factors and the experiences in the diagnosis and treatment of tumor lysis syndrome in patients with acute myeloid leukemia.Methods The clinical data of blood and biochemical tests were retrospectively analyzed in 84 patients with acute myeloid leukemia before and after induction therapy.Results Eight patients(9.5%)developed tumor lysis syndrome(3.6% clinical tumor lysis syndrome and 6.0% laboratory tumor lysis syndrome).The main biochemical features of tumor lysis syndrome were hyperkalemia,hyperuricemia,hyperphosphatemia,hypocalcemia and abnormal function of the kidney.The increased lactate dehydrogenase levels and high white blood cell counts were the risk factors for tumor lysis syndrome.Conclusion Tumor lysis syndrome is frequently observed in acute myeloid leukemia patients during induction therapy.The early diagnosis and treatment are the key to curing tumor lysis syndrome.

Association of adiponectin gene polymorphism with metabolic syndrome

Abstract: Objective To investigate the association of adiponectin gene(apM1) polymorphism with metabolic syndrome.Methods A total of 344 unrelated subjects were divided into metabolic syndrome group(n=180) and abnormal metabolism group(n=164) according to the degree of abnormal metabolism.Another 50 cases of normal metabolism were as controls.All these three groups were detected apM1 exon 2+45T→G and the apM1 exon 3+331T→C with PCR-RFLP technique.Results The polymorphisms existed at nucleotide +45 in exon 2 region in apM1 gene in Chinese Han people,and two alleles of T and G existed,consisting of three genotypes: TT,TG and GG.There were no significant differences in genotypic frequency and allele gene frequency among these groups(P0.05).There were no significant differences in the clinical characteristics,such as the concentrations of serum adiponectin,glucose and insulin between the subjects with allele gene G and the subjects without allele gene G(P0.05).There were no polymorphisms at nucleotide +331 in exon 3 region in apM1 gene in Chinese Han people,and the genotype was TT.Conclusion In Chinese Han people,the exon 2+45 polymorphism in apM1 is not correlated with metabolic syndrome,and the exon 3+331 polymorphism in apM1 gene does not exist.