Showing papers in "Plant Biosystems in 2023"

Micromorphological characterization of seeds of dicot angiosperms from the Thal desert (Pakistan)

Abstract: Abstract Micromorphological and ultrastructural data have been helpful in determining the evolution, classification, ecology and phylogeny of seed plants. Taxonomic utility of seed characters has been explored, although the value of micro-structure in selected dicot angiosperms from desert areas has not been adequately addressed. We conducted a comparative morphometric analysis of internal and external seed features in the 16 dicot species representing 10 families from the Thal desert (Pakistan) using scanning electron microscopy. Seeds were mostly minute and small and slightly larger than 1 mm in length except for Acacia nilotica, Astragalus hamosus and Prosopis juliflora. Seeds varied in shape from elliptical to obovate, D-shaped, reniform, rhomboid and ellipsoidal. The cell outline, periclinal boundaries and sculpture pattern of the anticlinal wall were generally rugulate, reticulate and striate, papillate and rugose or rarely undulating granulate. The seed coat also comprises diverse forms of epicuticular projections and texture. The phenetics of 84 character-states using principal component and dendrogram statistics supported the affinities among desert species. Here, we identified micromorphological similarities and differences among dicot angiosperms to determine their systematic relationships. Graphical Abstract

In vitro antibacterial activity of essential oils from Carum copticum and Ziziphora clinopodioides plants against the phytopathogen Pseudomonas syringae pv. syringae

Abstract: Abstract The present study aimed to determine the in vitro antibacterial activity and the chemical composition of essential oils (EOs) from two medicinal plants (seeds of Carum copticum L. and leaves of Ziziphora clinopodioides Lam) against the bacterial plant pathogen Pseudomonas syringae pv. syringae. The sensitivity of P. syringae to the two types of EOs was investigated through three methods including disc diffusion, well diffusion, and vapor phase tests. Gas chromatography (GC) and gas chromatography attached to a mass spectrometer (GC-MS) were used to identify and quantify the essential oil compounds. The greatest growth inhibition zones for disc diffusion, well diffusion, and vapor tests were observed for the highest essential oil volume (50 µL) of Z. clinopodioides as 0.925 cm, 1.75 cm, and 1.45 cm, respectively. Concerning the EOs from C. copticum seeds, the diameters of growth inhibition zones were 0.775 cm, 1 cm, and 0 cm, respectively. Based on the obtained results, the highest antibacterial activity, with a growth inhibition zone of 1.75 cm, belonged to 50 µL EOs of Z. clinopodioides on Luria-Bertani (LB) agar medium. The minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) for the EOs of Z. clinopodioides were 8 µL mL−1 and 1 µL mL−1 in LB agar medium. The MBC and MIC values for EOs of C. copticum were 16 µL mL−1 and 1 µL mL−1, in LB agar medium, respectively. According to the chromatography results, 16 different compounds were identified in the EOs of C. copticum, of which thymol (54.6%) was the major constituent. Moreover, 27 different compounds were identified in the Z. clinopodioides EOs, of which pulegone (23%) and 1,8 cineole (20.3%) were the main essential oil constituents. The results demonstrated that the EOs of Z. clinopodioides plants had higher chemical diversity and displayed stronger antibacterial activities against P. syringe pv. syringae, compared to those of C. copticum.

Modeling the influence of temperature, salt and osmotic stresses on seed germination and survival capacity of Stipa tenacissima L.

Abstract: Abstract This work stands for a modelling approach to identify the cardinal temperature of germination of Stipa tenacissima L., its capacity to recover and its adaptation potential considering dormancy and viability after exposure to salinity and drought. Seed traits characterised before germination were tested. The seeds were incubated at temperatures of 10 to 30 °C and low osmotic potentials were induced using sodium chloride (NaCl) or polyethylene glycol (PEG 6000) solutions of 0 to −1.6 MPa. The capacity of seeds to recover and remain viable under stress conditions was quantified. Our results proved that the seed yields and sizes were uniform. The analysis of cardinal temperature by thermal time model exhibited an optimal germination temperature (T o) of about 19 °C, a base temperature (T b) of 0.6 °C and a ceiling temperature (T c) of 31 °C at 0 MPa. The base water potential Ψb (50) values ranged between −0.83 and −1.54 MPa and between −0.97 and −2.27 MPa for osmotic and salt stresses, at 30 °C and 20 °C, respectively. In conclusion, the species showed a lower seed recruitment related to a low drought tolerance (drought tolerance index = −0.5 MPa), To requirement and threshold sensitivity. Under salt stress, S. tenacissima used osmotic adjustment in order to germinate in a lower osmotic potential.

First morphological and molecular investigation of the mycobiota associated with Astragalus nebrodensis in its natural environment (Madonie mountains, Sicily)

Abstract: Abstract Although Astragalus nebrodensis plays a fundamental ecological role, mycorrhizal fungi associated with this species have never been investigated in natural habitats. Our aim was to check the presence and occurrence of Arbuscular Mycorrhizal Fungi (AMF) in roots of A. nebrodensis in a natural habitat and to screen the fungal diversity in the surrounding soil, considering the positive influence of mycotrophic shrub species on soil microbiota. A morphological analysis was performed on A. nebrodensis roots samples from the Madonie mountains (Sicily, Italy), while high-throughput-sequencing was carried out in A. nebrodensis roots and in the associated soil. Observations of A. nebrodensis roots revealed typical structures of AMF. Sequencing revealed that Ascomycota was the most abundant phylum in both roots and soil samples, followed by Basidiomycota and Mucoromycota. A. nebrodensis roots host a fungal community with lower richness as compared to soil and specific taxa were differentially abundant between roots and soil. The endomycorrhizal symbiosis in A. nebrodensis from a natural habitat is reported here for the first time. The fungal diversity between the two matrices (soil vs roots) supports the notion of a specialised and well-established root microbiome in A. nebrodensis enabling it to survive the stressful conditions of its harsh environment.

Red-list of Italian bryophytes. 1. Liverworts and hornworts

Abstract: Abstract The objective of this study is to provide an updated conservation status of all liverworts and hornworts in the Italian territory, evaluated according to IUCN’s Guidelines for Application of IUCN Red List Criteria at Regional Level. Overall, 27.4% of taxa assessed in this study are assigned to a risk category and, therefore, considered threatened in Italy. Four liverworts are considered as Extinct, two liverworts and one hornwort Possibly Extinct at regional level. Moreover, a further 9.8% are assessed as Near Threatened, 10.8% as Data Deficient and 49.7% as Least Concern. Most threats are co-occurring, indicating that many taxa are subjected to a set of correlated adverse processes. The main IUCN major threats identified were natural system modifications (i.e. dam construction, increases in fire frequency/intensity, and water management/use), climate change (mainly increasing frequency of droughts and temperature extremes), agriculture (including pollution from agricultural effluents) and aquaculture.

Morphological and phytochemical features in fruits of Pimpinella anisoides V.Brig. (Apiaceae)

Abstract: Abstract The morphological characteristics, phytochemical composition of Pimpinella anisoides fruits was investigated. The morphological analysis of the monocarps contributed to distinguish P. anisoides from other related Pimpinella species used for commercial purposes, in particular P. anisetum and P. anisum. Our study showed that these fruits are different for their surface and shape: in particular, P. anisoides monocarps are glabrous, whereas the other two species are strigose. Two main compounds were identified in the essential oil of the five studied accessions: limonene (46.78-50.74%) and anethole (38.50-42.77%) whose sum ranged between 85.28 and 93.51%. estragole and β-phellandrene were other two representative compounds. All other volatiles accounted for less than 1% of the total volatile composition. One-way ANOVA analysis showed significant differences in almost all volatiles, whereas non-significant differences were found in the compounds detected in very low quantity. Hierarchical cluster analysis allowed to group the five accessions in three main clusters. Principal Component Analysis (PCA) showed that the first two principal components explained 71.80% of total variance. Results show that morphological and phytochemical features of the ripe fruits of P. anisoides can be used to distinguish this species from others, to avoid commercial sophistications and for a better conservation.

High temperatures during late floral bud stages decrease fertilization in strawberry (Fragaria × ananassa): pollen-pistil interaction and anatomical evidences

Abstract: Abstract High temperature (HT) effects on pistil tissues and female gametophyte have been scarcely investigated in crops species. HT in strawberry can induce fruit malformations due to poor pollen performance in pistils, reducing the fertilization level. In this study, Fragaria × ananassa cvs. Earlibrite and Fortuna were exposed to ambient temperature (AT) or HT at late flower bud development over the duration of 3 or 5 days. To evaluate the capacity of heated apical and basal pistils to support and guide pollen tubes, we examined the performance of unheated pollen grains and their path along the pistil, as well as the anatomy of reproductive tissues. HT significantly induced a decrease in the number of adhered and germinated pollen grains and of pollen tubes in the style and the ovule micropyle. Microscopic observations revealed loss of stigmatic papillae turgidity and fertilization failures due to abnormal pollen tube paths. The latter finding was related to the high incidence of immature female gametophytes in apical pistils and unviable female gametophytes in basal pistils. Facultative apomixis is also described. This is the first report of impaired pistil functions when strawberry buds are exposed to HT, as revealed by in vivo poor pollen performance 24 h after pollination.

Mixed inoculation of dark septate endophytic and ericoid mycorrhizal fungi in different proportions improves the growth and nutrient content of blueberry seedlings

Abstract: Abstract Despite extensive studies on the interaction between dark septate endophytes (DSE) and host plants, little is known about the effects of mixed inoculation of DSE and ericoid mycorrhizal fungi (ERMF) on plants. We assessed the effects of mixed inoculation of five species of ERMF and five species of DSE on soil factors and growth and physiological functions of blueberry (Vaccinium corymbosum L. ‘O’Neal’) seedlings. The strains were isolated from blueberries in several regions of Guizhou Province. The fungi suspension root irrigation method was used to inoculate the seedlings. Six inoculation treatments, i.e. D (Cladosporium cladosporioides), N (Oidiodendron citrinum), D1N1 (D : N, 1:1), D1N2 (D : N, 1:2), D2N1 (D : N, 2:1) and CK (not inoculated) were conducted. Samples were collected two months after inoculation. The mixed inoculation of DSE and ERMF significantly promoted the height, stem diameter, shoot dry weight, total biomass, root activity and total chlorophyll content of the blueberry seedlings and enhanced their absorption capacity to mineral nutrients. The mixed inoculation also improved the activities of soil enzymes such as urease and acid phosphatase. The effect of mixed inoculation was better than that of single inoculation or CK. Combining the analysis results of the membership function, we concluded that D1N2 and D2N1 most significantly promoted the growth of blueberry seedlings. These data provide a basis for the potential application of DSE and ERMF mixed inoculation for promoting the growth of seedlings.

Co-inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria can mitigate the effects of drought in wheat plants (Triticum durum)

Abstract: Abstract Drought stress (DS) is one of the severe abiotic stresses that cause reductions in agricultural production. This study was conducted to examine the efficiency of arbuscular mycorrhizal fungi (M: consortium) and plant growth-promoting rhizobacteria (R: Z2 and Z4 strains, B: CHIN14, AAP22, AAP2, and CHIN15 strains), individually or in combination, as an appropriate strategy to improve durum wheat productivity under two water regimes: well-watered (75% FC) and DS (25% FC). DS exhibition reduced biomass, stomatal conductance, stem water potential, and increased malondialdehyde and hydrogen peroxide content. Under DS, AMF or B promoted plant growth, such as shoot height by 49 and 17%, respectively; by activating the photosynthetic mechanism and increasing the synthesis of proline content by 369% in plants treated with AMF, and antioxidant enzyme activities, such as polyphenoloxydase (PPO), in leaves by 36% in plant inoculated with B. After the experiment, total organic matter and carbon of the soil were improved by single application of B by 99 and 317%, respectively. This finding implies that the combination of mycorrhiza and PGPR regulates the physiological and biochemical processes utilized by wheat to cope with drought and improves the comprehension of the soil-plant relationship.

Ecological and syntaxonomical characteristics of early spring therophytic ephemeral grasslands (alliance Romuleion) in the northeastern Mediterranean

Abstract: Abstract Our study deals with dry grasslands dominated by Romulea bulbocodium, Romulea linaresii subsp. graeca and Romulea columnae that develop in early spring in the eastern Mediterranean region. Based on numerical classification, we describe two new associations: Romuleo bulbocodii-Poetum bulbosae ass. nova and Ornithogalo exscapii-Poetum bulbosae ass. nova. The studied communities are classified within the Romuleion alliance, order Poetalia bulbosae, and class Poetea bulbosae. We analyzed all available relevés of Romuleion communities from the eastern Mediterranean and ordination analysis (NMDS) revealed that temperature and altitude are the most important ecological factors influencing the vegetation composition and distribution of dry grasslands of the Romuleion alliance. Newly described associations from Montenegro are floristically and ecologically similar to the Alyssum alyssoides-Poa bulbosa community from Epirus (Greece).

The bryophyte vegetation of gypsum outcrops: a focus on communities with Tortula revolvens

Abstract: Abstract This paper describes a bryosociological study on the gypsicolous bryophyte communities of the Mediterranean area and central Europe, characterized by Tortula revolvens, the only bryophyte that is strictly gypsophytic. The cluster analysis has emphasized the occurrence of four associations belonging to the class Psoretea decipientis, well differentiated as concerns the ecological requirements and floristic composition; they are: Tortuletum revolventis and Trichostomo crispuli-Tortuletum revolventis of the order Barbuletalia unguiculatae, Crossidio crassinervis-Tortuletum obtusatae and Trichostomopso-Tortuletum obutasatae of the order Tortulo brevissimae-Aloinetalia bifrontis. Trichostomo crispuli-Tortuletum revolventis is here described as new. Considering the ecological requirements, type of gypsum outcrop and topographic location, it is possible to identify three distribution models: a first model with distribution in dry areas of central Europe and in Sicily, a second one with core distribution in the driest areas of southern Spain, and a third one in the semidesert and desert areas of Tunisia, Jordan and Israel.

Contribution to the knowledge of genome size variation in Calendula L. (Asteraceae) with special focus on the SW Mediterranean region

Abstract: Abstract Calendula is one of the most taxonomically complex genera within the Asteraceae family due to hybridization, polyploidization events, and production of a highly variable morphology of the achenes. Considering the complexity of Calendula, this study was conducted to extend the understanding of the relationships between SW Mediterranean taxa, and assess the relationships between genome size and chromosome number, ploidy level and life cycle in 77 populations covering 14 taxa. Genome size estimations are provided for the first time for five species and for three putative new undescribed species. Mean 2C values differed up to 6-fold among different euploid species (from 1.37 pg in diploid to 8.26 pg in octoploid populations). 1Cx-values varied 2.07-fold (between 0.68 pg and 1.41 pg). Mean 1Cx genome size revealed significant differences between different ploidy levels. Genome size variation is a significant factor for explaining the relationships within Calendula, and individuals not fitting the current classification were found and should be analysed in detail in future studies.

Tropical terrestrial and epiphytic ferns have different leaf stoichiometry with ecological implications

Abstract: Terrestrial and epiphytic herbaceous forest species have different ecological qualities and leaf stoichiometry. Ferns represent a great component of herbaceous forest species in tropical regions with different lifeforms and evolutionary histories. However, little is known about the differences in leaf stoichiometry between the lifeforms. We studied the concentrations of leaf elements (N, P, K, Ca and Mg) between lifeforms (16 terrestrial, 4 epiphytic) and evolutionary clades (12 polypods, 8 non-polypods). The leaves were collected from the field, acid-digested and analyzed for nutrient elements. Results show that epiphytic species had a higher concentration of most leaf elements. Epiphytes had a lower N:P ratio compared with terrestrial ones. This suggests that epiphytes were nutrient-limited, relying on stochastic elements supply compared to terrestrial species which have a constant supply. Epiphytes tend to accumulate higher element concentration beyond their immediate metabolic needs, defined as luxury consumption. Epiphytes showed a higher concentration of P and Ca for their necessity of coping with severe habitat conditions and sclerophyll leaves. We found that modern polypod and archaic non-polypod ferns are significant stoichiometry different for K. The results contribute to the context of the poorly studied stoichiometry of tropical Asian fern species and their ecophysiology.

Antifungal and antiparasitic activity of Wunderlichia azulensis (Asteraceae) roots

Abstract: Abstract The aim of this work was to evaluate the antifungal and antiparasitic activity of roots of Wunderlichia azulensis (Asteraceae). A CH2Cl2 partition of W. azulensis was tested against Candida tropicalis, C. buinensis and Trypanosoma cruzi parasites. A chemical study of the CH2Cl2 partition resulted in the isolation and identification of dehydrobrachylaenolide (1), lasiodiplodin (2), a taraxerane-type triterpene (3) and a mixture of sitosterol and stigmasterol (4–5). Of the 13 fractions tested, fractions 5 and 7 were the most bioactive ones. No live epimastigotes were observed even after the shortest time of exposure to fraction 7 (25 μg.mL−1/24 h). Fractions 5 and 7 inhibited yeast growth in proportions between 63.9–80.7% at 50 μg.mL−1, and 70.3–96.7% at 100 μg.mL−1. Fractions 5 and 7 (100 μg.mL−1) induced loss of viability of C. buinensis by 97.61 and 92.63%, respectively, whereas C. tropicalis was less susceptible. The SYTOX Green marker used on Candida yeasts suggested membrane permeabilization and consequently plasma membrane damage. We concluded that W. azulensis offers new sources of biologically-active components. However, complementary studies on other variables, such as synergy, toxicology, and mechanism of action, must be carried out.

Chemical sensitivity phenome of an Armillaria mellea isolate determined using phenotype microarrays and potential use of caffeine-rich wastes for disease control

Abstract: Abstract Armillaria fungi are frequently documented as economically and ecologically significant plant pathogens, recognised as the causal agents of Armillaria root rot disease. Armillaria mellea is one of the most aggressive pathogens in the Armillaria genus. None of the chemicals tested against Armillaria have been effective in fully eradicating an established A. mellea mycelium from an infection site and/or preventing plant mortality, which makes studies about the chemical sensitivity of A. mellea essential. In this work, the inhibitory effects of 120 different chemical agents on the growth rate of A. mellea were examined using the Biolog Phenotype MicroArray system of chemical sensitivity panels. Among the tested substances, aromatic and membrane function compounds showed the highest inhibitory activity against A. mellea. Interestingly, our results demonstrated promising potential for application of caffeine as an A. mellea-oriented fungicide. Further studies were conducted to explore the antifungal activity of a low-cost and locally available caffeine-rich waste, i.e. spent coffee grounds (SCG) against A. mellea. It is noteworthy that the hyphal growth of A. mellea was significantly inhibited when cultivated on malt extract agar supplemented with SCG. Current findings uncovered, for the first time, the potential use of caffeine-rich wastes for designing management strategies to practically control the spread of A. mellea.

In vitro antioxidant and cytotoxic effects of three endemic plants from Turkey based on their phenolic profile

Abstract: Abstract This study aimed to determine in vitro antioxidant and cytotoxic activities of acetone and water extracts obtained from Helicrysum noeanum, Onosma bozakmanii, and Sideritis amasiaca. We tested their total phenol and flavonoid content and analyzed their 14 phenolic compounds using HPLC method. Cinnamic acid, gallic acid, protocatechuic acid and quercetin were phenolics found in all extracts. Acetone extract of S. amasiaca was the experiment containing the most phenolic compounds by containing 11 of them. While the highest total phenol content belonged to water extract of S. amasiaca (189.37 µg gallic acid equivalent/mg extract), water extract of O. bozakmanii had maximum total flavonoid rate (13.16 µg quercetin equivalent/mg extract). Acetone and water extracts of S. amasiaca showed higher DPPH scavenging (IC50: 43.40 and 18.71 mg/L, respectively) and metal chelating (IC50: 62.26 and 61.32 mg/L, respectively) activities compared to other plants. When viability rates of human cervical cancer cells (HeLa) treated with the extracts were investigated, the experiment with the highest cytotoxic effect was acetone extract of S. amasiaca (IC50: 45.10 mg/L). Overall, our data showed that H. noeanum, O. bozakmanii and especially S. amasiaca are important natural antioxidant sources. They can be used in pharmacology thanks to their essential phytochemicals.

Morphological diversification of Teucrium montanum sensu lato on the Balkan Peninsula

Abstract: Abstract In this work, the overall morpho-anatomical variability of Teucrium montanum sensu lato on the Balkan Peninsula was described. A total of 60 populations were collected all over the Balkans. For each analysed individual, the morphological characters of the stem, leaves, and flowers, as well as the anatomical characters of leaves were measured. The habitats of each population were characterized by altitude zone, type of geological substrate, vegetation, and climate type. Basic descriptive statistics were calculated for each character state, and multivariate analyses were used to compare all assessed characters and groups. The results showed that the analysed populations exhibit high morphological variability and broad ecological preferences. Five of the seven morphologically defined groups fit the already described taxa (T. montanum subsp. montanum, T. montanum subsp. helianthemoides, T. pannonicum, T. skorpili, T. montanum var. parnassicum), whereas two groups are morphologically specific but did not fit the existing taxonomic concepts. The patterns of observed morphological variability only partially match the patterns of ecological differentiation of the habitats of the analysed populations. The occurrence of different morphological groups inhabiting similar habitats in the same biogeographic region suggests that the morpho-anatomical differentiation of T. montanum s.l. may be due to genetic or evolutionary factors.

Leaf morphoanatomical character variation of Gyrinops and Aquilaria (Thymelaeaceae) in Indonesia region at east Wallace line

Abstract: Abstract There are seven species of Gyrinops and two species of Aquilaria (Thymelaeaceae), which are distributed in Eastern Indonesia at the east of the Wallace line. The anatomical character of the paradermal tissue, midrib and petiole were the important characteristics possessed by plants that can be used as data for identification. The purpose of this article was to study leaf morphoanatomical character variation of Gyrinops and Aquilaria in Indonesia Region at East Wallace Line. The Gyrinops specimen used in the research was the collection of the Agarwood Study Center, Universitas Mataram, Mataram, Indonesia. The paradermal slide was made with the whole-mount slide method and the slide cross-section of midrib and petiole was made by using a hand-free section. Their preparation process used a permanent slide method and mounted with glycerine jelly. Based on the results of the research conducted, it can be seen that the anatomical characters of paradermal tissues, midrib and petiole of Gyrinops and Aquilaria have general characteristics such as hypostomatic leaves, stomata type anomocytic, non-glandular and unicellular trichome. While other characters of structure paradermal, midrib and petiole could be used as a taxonomy diagnostic marker to identify at the level genus and species on Gyrinops and Aqilaria.

Lavandula austroapennina and Lavandula angustifolia essential oils and bioactive components: in vitro anti-denaturation effect of lavender from the Pollino massif (Southern Italy)

Abstract: Abstract The Mediterranean basin represents a rich source of medicinal and aromatic plants. These species contain different metabolites such as phenolics, flavonoids, tannins, coumarins and sterols known for their beneficial properties. The essential oils (EOs) of Lavandula austroapennina and Lavandula angustifolia from the Pollino massif (southern Italy) were characterized using gas chromatography-mass spectrometry and their anti-denaturation effect was assessed using in vitro models with heat-treated bovine serum albumin (BSA) chosen as a protein model. L. austroapennina EO showed a better in vitro anti-denaturation activity compared to L. angustifolia, with IC50 values equal to 260.4 ± 4.2 and 480.0 ± 2.6 µg/mL, respectively. In order to relate the observed results to the most interesting identified phytochemicals, some major components were also tested. Linalool and terpinen-4-ol proved to be effective in protecting BSA from heat denaturation. Moreover, the inhibitory properties on nitric oxide production were verified in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both lavender EOs showed concentration-dependent inhibitory properties, as well as the monoterpene linalool. Taken together, these results suggest that L. austroapennina EO and its main constituent linalool could be good candidates for further investigations, aimed at finding new drugs with anti-arthritic potential.

Cathissa villasina (Hyacinthaceae), a new endemic species from the southeastern Iberian Peninsula

Abstract: Abstract A new species, Cathissa villasina, endemic to the Sierra de Las Villas (Jaén province, Eastern Andalusia, Spain), is described, illustrated and compared to its closest relative (C. reverchonii), with which it was initially confused in this area. A comparative morphometric study (quantitative and qualitative) of both species including a statistical analysis of 39 macroscopic characters and the detailed microscopic observation of stigma, seminal testa and pollen was performed. In terms of its ecology and phytogeography, C. villasina grows in pockets of clayey soils on limestone pavements on terrain with little or no incline. Humidity levels in these soils are high and they can become temporarily waterlogged. This species is a narrow endemic to the Cazorlense biogeographical district. By comparison, C. reverchonii is clearly a rupicolous species that grows in the shade of vertical or steeply sloping limestone rocks, and is found in numerous scattered localities in the biogeographical Rondense district.

Phylogenetic analysis of Ziziphora (Lamiaceae) using nuclear (nrITS) and plastid (trnL-F) DNA: suggestions for new arrangements and status changes

Abstract: Abstract In this study, Ziziphora taxa grown in Turkey along with those grown in different parts of the world were analyzed with respect to morphological revision and molecular systematic analyses using nuclear ribosomal ITS (nrITS) sequences and the chloroplast (trnL-F) sequences. Both nrITS and trnL-F sequences clearly suggested Ziziphora was a monophyletic genus. Morphological revision mostly confirmed the previous systematic position of the taxa examined, while for some taxa, morphological uncertainties combined with DNA sequence data suggested rearrangements and new taxa. With this respect, some of the unidentified Z. clenopodioides specimens were suggested as potentially 2 new subspecies while the status of Z. clenopodioides subsp. filicaulis was suggested to change from subspecies to species. Interestingly, four Z. tenuior ecotypes from the eastern, central and southern parts of Turkey displayed different nrITS sequences and hence they were suggested as four different new subspecies of Z. tenuior. This is the first report presenting a revised status of Ziziphora L. taxa in Turkey based on molecular systematic analysis, with respect to its related members in different parts of the world.

Predicting the suitable habitats of Elwendia persica in the Indian Himalayan Region (IHR)

Abstract: Abstract Rare, Endemic and Threatened (RET) species with naturally small populations are always at high risk of extinction, especially during the climate change process. Climate change phenomena are also identified as a strong driver in the habitat shift of many medicinal and aromatic plants (MAPs). The expected consequences of climate change are so dangerous that some key species can move to extinction. Therefore, the quest for suitable habitats of such species is taken as a challenge by various ecologists and conservationists. This study aims to predict the suitable habitats of Elwendia persica (Boiss.) Pimenov & Kljuykov, a threatened species for current and future climatic scenarios in the Indian Himalayan Region (IHR). The assessments for current and future climatic scenarios are accessed on the optimistic Representative Concentration Pathway 4.5 (RCP 4.5). The MaxENT algorithm has helped to predict the suitable habitat of the species in the study area. The model has predicted 1.12%, 2.37%, and 0.98% of the total study area as highly suitable habitats in current (2000) and future (2050 and 2070) climatic scenarios. South-eastern facing slopes are considered as the most suitable areas for the species in the Indian Himalayan Region. Our results show that suitable habitats of the species may increase upto 2050, and subsequently decrease.

Afforestation of semi-arid regions of Mongolia: carbon sequestration in trees and increase of soil organic carbon

Abstract: Abstract This work examines the variation of carbon sequestration (considered as the sum of above- and below-ground plant biomass production added to soil organic carbon) occurring in two sites (named respectively BN and ER) characterized by a semi-arid climate after planting three tree species (Populus sibirica, Ulmus pumila, and Hippophae rhamnoides). Soil organic carbon (SOC) was analyzed at four different depths (from 0–60 cm). In these two sites, the three tree species contributed differently to carbon sequestration. We found that above-ground plant biomass (leaf, branch, stem) was higher at the BN site whereas at the ER site prevailed below-ground biomass. That is probably due to the low tree density in BN site and the higher soil moisture content present in ER site. Among the three tree species considered, P. sibirica showed the highest biomass value. The SOC values were highest in the topsoil layer (0-40 cm) and decreased with depth. This works clearly shows that in semi-arid lands carbon sequestration depends on the environmental factors that characterize the site, the tree density, and the tree species selected.

Phytosociological analysis of the endemic Quercus faginea forests of the Iberian Peninsula

Abstract: Abstract A phytosociological analysis of the Iberian Quercus faginea forests was carried out. For this purpose, 291 relevés were studied. A multivariate analysis consisting of a numerical classification based on the floristic composition of the relevés, was performed. The coherence and explanatory value of the current syntaxa are reviewed. As a result, the diversity of Iberian gall oak forests is resolved into 15 associations characterised according to their floristic, biogeographical, bioclimatic and synecological data supported by the statistical analyses. Thirteen have been described previously, and two new associations are proposed: Glandoro diffusae-Quercetum fagineae and Helianthemo mollis-Quercetum fagineae. A new combination, a lectotypification and a nomenclatural correction are also proposed. The Ios2 (ombrothermic index of the warmest bimonth of the summer quarter), Itc (compensated thermicity index) and Ic (continentality index) indexes proved to be fundamental to explain the syntaxonomic differentiation of the forests in the study. We consider that the differentiation and precision in the definition of clear syntaxonomic entities is vital to prioritize objectives in the conservation of these singular forests, and to ensure their adequate management in the context of the Habitats Directive.

Tree tracking: species selection and traceability for sustainable and biodiversity-friendly urban reforestation

Abstract: Abstract The current climate change and increasing climate variability represent a serious global challenge that demands urgent responses. Among the multiple actions that should be taken, afforestation and reforestation are the most common strategies. The current paper aims to assess the potential of tree species to be used in urban forestry in the Po Plain ecoregional province (Northern Italy) using DNA barcoding approach for plant traceability and ecophysiological indexes (shade, drought and waterlogging) for plant selection. DNA barcoding markers results showed a 55.5% of success, but the power of discrimination increased to 94.5% when considering only the autochthonous ecoregional species pool. Concerning ecophysiological markers, Fagus sylvatica, Abies alba, Picea abies and Taxus baccata were very shade-tolerant. The best performing trees concerning drought tolerance were oak species (Quercus ilex, Q. pubescens and Q. cerris), pines (Pinus pinea, P. nigra and P. sylvestris) and Fraxinus ornus. Only a few tree species (Salix alba, Alnus glutinosa, Populus nigra and Prunus padus) showed high tolerance to waterlogging. The combination of both approaches makes it possible to select the most suitable species, based on eco-pedological and microclimatic conditions, and to trace their identity along the entire supply chain, from nursery to planting, for urban forestry. GRAPHICAL ABSTRACT

Origanum minutiflorum: phytochemical profile and inhibitory effects on key enzymes associated with inflammation

Abstract: Abstract Origanum minutiflorum, an endemic species in Turkiye, is used for both food and medicinal purposes. 70% ethanol extract of aerial parts of O. minutiflorum was subjected to liquid-liquid extraction using; n-hexane, dichloromethane, ethyl acetate and n-butanol. The inhibitory activity of these fractions on lipoxygenase (LOX), cyclooxygenase (COX) and matrix metalloproteinase-9 (MMP-9) was investigated at 100 µg/mL concentration. The LOX enzyme was most effectively inhibited by the dichloromethane fraction (82.33%), whereas the COX-1, COX-2 and MMP-9 enzymes were most effectively inhibited by the ethyl acetate fraction (78.50%, 83.96% and 45.11%, respectively). The ethyl acetate fraction was selected for activity-guided fractionation based on the COX enzymes’ inhibitory activities by column chromatography. Only the ethyl acetate sub-fraction-1 showed inhibition on the COX-1 with 65.78%. The COX-2 inhibitory activities of the sub-fractions ranged from 59.96% to 97.62%. The main components were determined by HPLC-MS/MS as jaceidine in the dichloromethane fraction and rosmarinic acid in the ethyl acetate fraction. As far as we know, this study is the first to correlate the anti-inflammatory activity of O. minutiflorum extracts with LOX, COX and MMP-9 enzyme pathways and their secondary metabolites. These data showed that O. minutiflorum may contribute to other studies on natural origin anti-inflammatory agents.

Mitigation of salt stress and stimulation of growth by salicylic acid and calcium chloride seed priming in two barley species

Abstract: Abstract The main objective of the current study is to underline the effects of salicylic acid or calcium chloride seed priming on plant growth, water content, chlorophyll content and photosynthetic activity of two barley species Hordeum marinum and Hordeum vulgare exposed to salinity (0, 100 and 200 mM NaCl). Results revealed that salinity decreased shoot and root growth, transpiration rate, chlorophyll concentration, stomata conductivity and water content in plants obtained from unprimed seeds. This reduction was dependent on the saline concentration used. However, Hordeum marinum species showed tolerance by maintaining tissue hydration, growth, photosynthetic assimilation and chlorophyll biosynthesis in stressed plants compared to controls. Moreover, priming seeds with salicylic acid or calcium chloride significantly improved all the above parameters under different salt treatments, suggesting that the effectiveness of this approach mainly depends on the priming agent applied and may vary with species and stress intensity applied.

Neotropicomus australis var. porulosus var. nov. (Agaricomycetes, Boletaceae), a new bolete from “brejo de altitude” forest in Brazil

Abstract: Abstract Neotropicomus australis var. porulosus is characterized macroscopically by the small basidiomata, the dark reddish brown, slightly viscid and scrobiculate pileus with unchanging context, the greenish and unchanging hymenophore with small (≤0.5 mm in diam.) pores and the brownish stipe with unchanging context, creamy towards the base and fibrillose. Microscopically, the variety is characterized by pale to slightly dark yellowish basidiospores of 11.7–13.3(–13.8) × 4.1–5.1 µm, a pileipellis as epithelium consisting of erect to suberect then sometimes very slightly intertwined chains of aggregated globose, subglobose to ellipsoid cells, a phylloporoid tube trama, subparallel to barely divergent, and some caulocystidia with apex bearing an elongate cylindrical projection. The new taxon is described here from the Caatinga biome, in a ‘brejo de altitude’ forest in the states of Paraíba and Pernambuco. Thus, this is the first record of this genus from northeastern Brazil. We also propose here a modification of the genus to include small-pore specimens, and a new combination of Xerocumus globuliger with Neotropicomus.