Showing papers in "Southwest China Journal of Agricultural Sciences in 2007"

Single spore strains without producing fruit body isolated from Cordyceps militeris and their RAPD analysis

Abstract: 6 single spore strains without producing fruit body were isolated from the original Cordyceps militeris,WWM04 with producing fruit bodyThe genetic diversity of SSP2,SSP7,SSP19 and SSP21 strains without producing fruit body,the original strain and the strain after 12 transferred from the original strain was analyzed by the RAPD methodThe results show that the primers S37 and S61 are of apparently specific to the amplified bands of six strains,which establishes the basic for the degenerate molacular marker of Cordyceps militeris and confirms heterokaryosis and parasexuality of Cordyceps militeristo some extent

Investigation of geographical distribution and evaluation of Jatropha curcas in Yunnan province

Abstract: The distribution,resource volume,content of crude fat of Jatropha curcas were systematically investigated and studied in Yunnan province.The result showed that the resource of Jatropha curcas are mainly sporadically distributing in 260 countryside of the watershed of Jinshajiang,Lancanjiang,Nujiang and Honghe in Yunnan province.There was greatly difference in crude fat among 81 populations and individuals.The contains of crude fat in the 81 populations,the Yongren county contained 67.00 % and the Pingbian county contained 34.88 %.The resources status and prospect of Jatropha curcas exploration and utilization in Yunnan province was analyzed.

Research advance on Banded Leaf and Sheath Blight of maize.

Abstract: Banded Leaf and Sheath Blight were becoming the serious and major diseases in main maize production region of ChinaThis paper reviewed the recent advances of researches on the disease symptom,pathogens,nosogenesis,occurrence rule,penetration paths,damage,preventive treatment and inheritance of resistance to Banded Leaf and Sheath Blight in maizeWorks on Marker-assisted Selection(MAS) for Banded Leaf and Sheath Blight in maize were also summarized

Investigation on fattening and carcass traits in Tibetan pig and its combinations.

Abstract: Four pig breeds including Tibetan Pig,Chenghua Pig,Duroc and Pietlan were chosen,and 8 combinations(ZZ,ZC,CZ,DZ,DCZ,DZC,PZC PCZ) were set in present study.Fattening and carcass performances of 64 pigs(8 heads from each combination) were measured.The results showed that PCZ and PZC had higher average daily gain,feed conversion rate,growth rate and lean meat percentage,but poorer color scoring and lower intramuscular fat content(IMF).Although the fattening and carcass performances of DCZ DZC were a little lower than that of PCZ PZC,the latter had better color scoring and higher IMF,DCZ DZC were better combinations in the study.

A study on the benefit of feeding Hy-line white egg with fermented feed.

Abstract: The experiment aims at finding out what role EM will play on content of cholesterol in eggs and what role EM will play on the immune of chickens..Feed fermented fully with EM was mixed with the unfermented feed with percent of 20%,30%,40%,50%,60%,70%,80%,90%,100% to feed Hy-Line White Egg variety.The control was feed by unfermented feed.The results showed that the content of fat and cholesterol of the 80%,90%,100% groups were significantly lower than those in control group(P0.05);the content of protein,dry ingredient and weight of eggs have no differences among all groups;the content of fat and cholesterol in feed fermented were significantly lower than unfermented feed and the antibody and T.cell of the 50%-100% groups were significantly higher than those in control group(P0.05).The immune organ of chickens has no difference among all groups,it is different with other people's experiment.But if the percent of the ferment is too high,the output of egg will descend.

Utinization of CIMMYT germplasm and breeding technologies in wheat improvement in Sichuan, China.

Abstract: In 1966,the first CIMMYT released wheat variety Penjamo 62 was introduced into Sichuan;in 1989,the agreement of China-CIMMYT wheat shuttle breeding collaborative project was signed in Beijing and Sichuan was designated one of the three spring wheat shuttle sites in China.Since then,wheat breeders in Sichuan started to use CIMMYT germplasm and breeding technologies regularly for improving wheat breeding in Sichuan.In this long period of cooperation with CIMMYT,we realized that the very important and helpful things we got from CIMMYT in our wheat improvement in Sichuan were basically three areas:the exchange of useful breeding germplasm;the offering of new breeding strategies and technologies;and the exchange of scientists,especially the training course for young breeders from Sichuan.What we should do and how we could do to use these CIMMYT germplasm and advanced technologies in improving wheat breeding work in Sichuan? Our basic experience are as followings:(i)screen introduced CIMMYT germplasm under Sichuan ecologic environments intesively;(ii)based on the breeding programs in our academy and breeding materials and breeding systems they have,several CIMMYT basic,new and advanced breeding technologies and strategies were chose,such as winter/spring,shuttle breeding,modified-bulked selection,and multi-minor genes durable resistance of yellow rust,to improve and form a more effective breeding system in Sichuan with the DH population method and RILs we learned from a China-ACIAR Sichuan wheat improvement project;(iii)because the big difference of ecologic environments and the genetic background of germplasm between CIMMYT and Sichuan,we emphasize that introducing is just for improving and creating our own new breeding system,for example,we introduced winter/spring method from CIMMYT but now we modified it into winter/spring//spring,or CIMMYT/Sichuan//Sichuan and these were indicated very helpful breeding pattern in using CIMMYT germplasm in Sichuan;(IV)formed a scientific research team based on the wheat breeders who know both CIMMYT wheat breeding and Sichuan wheat breeding very well,especially the young trainees backed from CIMMYT training curse,this is a very important work base for continuing,insistant and successful cooperation with CIMMYT;and(V)strengthen the exchange of scientists and training the young wheat breeders in CIMMYT to enhance the understanding of scientists between CIMMYT and Sichuan in their breeding philosorph,breeding strategies,ecologic environments,their breeding materials and the growth of breeding materials in field,and in this way the scientists of both side can help each other and therefore making the cooperation more effective and smoothly.By year 2006,19 CIMMYT germplasm derived wheat varieties were released in Sichuan,among them 4 were also released by national level and one was the first commercial common wheat variety in the world derived from a CIMMYT synthetic hexaploid wheat.These new released CIMMYT germplasm derived wheat varieties were performed very well in Sichuan in their resistance to stripe rust by incoporated some new resistant genes,the broken through of breeding for high quality by releasing of the first strong gluten varieties in Sichuan,and the broken through of breeding for high yield potential indicated by a new record of yield of 6.13 t/hm2 in provincial yield trial by new variety chuanmai 42.It is expected that in near future there will be more CIMMYT derived new wheat varieties to be released in Sichuan.All of these give us a confident information that CIMMYTgermplasm and breeding strategies and breeding technologies are now one of the very important factors to create new technologies and develop new type of wheat varieties in Sichuan.For future,the collaboration of Sichuan-CIMMYT on wheat breeding should be continued and strenthened.Recent years,some quite new and more interesting research areas in improving wheat breeding in both side are iditified by CIMMYT and Sichuan scientists,for example,(i)the development of new breeding materials of high yielding potential with good adaptable type and their genetic studies(may be based on Chuanmai 42);(ii)studies and new varieties development on durable stripe rust resistance in Sichuan,and in particularly the exploitation of new resource of durable resistant new genes(based on present research and some core-germplasm in Sichuan);(iii)screening and developing new breeding materials and varieties of stable performance on high quality characters(particularly for high quality noodles) under Sichuan ecological environments;and(IV)exploiting the new cultivation systems for high yield with high quality under more saving,easy operating and more environment protection.To ensure this cooperation could be continuing insistently and maintain more active,more efficient and more fruitful,it is especially important that the training of young scientists from Sichuan in CIMMYT and the exchanging of scientists through some sepcial studies and programs should be continuing and strethening.

Mechanism of ethephon on increasing cane yield and sucrose % cane in sugarcane.

Abstract: A series of investigations was conducted at levels of population,plant,organ,cell and molecule to illustrate the mechanism of ethephon on increasing cane yield and sucrose % cane in sugarcane.It was found that the ethephon treatment first induced the expression of the genes encoding ACC synthase and ACC oxidase and resulting in an ethylene releasing peak inside the tissues,which changed balances of the five endo-hormones,which affected the expression of various functional genes and so affecting the division,elongation and differentiation of the cells that leaded to the direct changes in the development of organs and tissues and the growth of the plants.The increases of peroxidase and IAA oxidase activities induced by foliar spray of ethephon promoted the oxidation and degradation of IAA,which led to the decrease of IAA in the tissues so resulting in temporary inhibition of the growth of the above-ground part of the plants.However,the relative high concentration of ethylene speeded up the synthesis of IAA inside the tissues,which explained the fast growth after a short inhibition.If the applied ethephon concentration is too high,the plant growth would be inhibited for a long time.However,this situation would be good for sugar accumulation in the stalks during sugar accumulation stage,so ethephon could be used as the ripener of sugarcane.

Study on the distant hybrid utilization between Saccharum and Erianthus arundinanceus

Abstract: Progress of distant hybridization utilization between Saccharum and Erianthus arundinaceus were reported in this paperErianthus arundinaceus F1 and BC1 and BC2 real hybrids have been obtained through crossing and backcrossing between Erianthus arundinaceus and Erianthus arundinaceus F1 and BC1 and Saccharum officinarum or cultivaresThe results showed that setting percentage of Erianthus arundinaceus F1 and BC1 was low,their fertile was low and infertile,but low setting percentage of Erianthus arundinaceus F1 and BC1 was improved by backcrossing,and Erianthus arundinaceus BC1 and BC2 hybrids of backcrossing showed stronger vigor and high yield and sucrose abilitiesIt should be established well bases for breeding high yield and sucrose sugarcane clones with consanguinity of Erianthus arundinaceus

Studies on the species of flower thrips and its occurrence in Dounan Chenggong county of Yunnan.

Abstract: The paper studied on the thrips species on 5 kinds of flowers,that were Carnation,Rose,Gypsophilag,Lily and Calla in Dounan,Chenggong county of yunnan province384 thrips specimens were collected and prepared on slide,and 6 thrips species were identified,that were Aeolothrips fasciatus,Frankliniella occidentalis,Frankliniella intonsa,Thrips flavus,Thrips hawaiiensis and Thrips tabaciThe identification characters were also described in detailFrankliniella occidentalis and Frankliniella intonsa were the predominant thrips species on the flowersThe results of field investigation indicated that,thrips occurred all seasons a year,and occurence fastigium ranged from April to JulyThrips could be found in every greenhouse when the damage was seriousThrips population decreased from August along with the temperature decreasing

Primary studies of acarocidal activity of twenty plants extracts against Panonychus citri.

Abstract: The bioactivities of ethanol extracts from twenty plants against Panonychus citri were measured in the laboratory with the glass plate dipping to find the strongest acaricidal plant.The results demostrated that Boenninghausenia sessilicarpa,Laggera pterodont,Humulus scanden and Rabdosia of ethanol extracts,of which LC50 were 0.9241,0.9827,0.9905 and 1.0196 mg·mL-1,respectively.The extracts of B.sessilicarpa possessed significantly ovicidal activity four extracts,of which LC50 was 1.7032 mg·mL-1.

Investigation and protection countermeasures for wild rice resources in Guiping, Guangxi.

Abstract: Two species of wild rice resources,Oryza rufipogon and Oryza officinalis,originally distributed in Guiping,Guangxi.Resources of wild rice in Guiping were investigated and recollected in May of 2007.The results showed that 2 new distribution sites with an area of 1998 m2 in original places were found.24 existing distribution sites of Oryza rufipogon accounted for 42 % of original records,which showed that 58 % of them were destroyed.2 existing distribution sites of Oryza officinalis accounted for 14 % of original records,which showed that 86 % of them were destroyed.The reasons that led to the serious destruction of wild rice resoures were land reclaim of agriculture,over herding,interruption by exotic species and coal mining.These protection countermeasures were put forward,including deepening the whole nation's protection understandings to wild rice resources,establishing in-situ protection areas of wild rice,strengthening conservation and etc.

Genetic diversity of tea camellias germplasm by ISSR molecular marker.

Abstract: ISSR molecular marker were used to analyze the genetic polymorphism of Sichuan-Chongqing tea camlias germplasm.The results showed that the genetic polymorphism of these materials were abundant.100 bands were amplified with 12 ISSR primers,and 90 bands of these were polymorphic ones(about 90 %).The genetic similarity of the materials varied between 0.41 and 0.87.The 14 materials were classified into 2 groups with UPGMA method,the first group is original arbor and large-leaf type,the second one is little-arbor or shrub type,and can be classified into 4 subgroups,mainly the cultivars of Sichuan-chongqing region.The results suggest that ISSR marker can be effectively used to evaluate the genetic polymorphism of tea camellias,and provide the foundation for the systematic research.

Relationship between starch saccharification and propagation of bulblets from scales in oriental hybrid lily (Lilium L.).

Abstract: The bulbs of three oriental lily varieties were stored in low temperature conditions for different time respectively.The relationship among soluble sugar content of bulbs,the number of propagation and perimeter of the bulbscales were analyzed.The results show that there is a degree of positive relativity between soluble sugar content and the number of propagation in bulbscales.With the increase of storage day,the number of propagation will keep a approach balance among different varieties,but the perimeter of bulbscales has a decline trend.

Relationship between SPAD readings chlorophyll contents and yield of potato(Solanum tubersosum L.)

Abstract: SPAD readings and chlorophyll contents in leaves of the first generation of crossed combination true seed potato were measured at bud stage,then the correlation between SPAD readings chlorophyll contents and single yield were analyzed.The results showed that correlation between SPAD readings and Chla,Chlb,Tchl,single yield are significant at 0.01 level respectively and the regression equation are: y=0.0472x-0.8102(r=0.8304);y=0.013x-0.1848(r=0.7424);y=0.0601x-0.9948(r=0.8221);y=23.22x-736.76(r=0.496),respectively.The conclusion showed that SPAD readings can fully reflect the chlorophyll contents and single yield,further more the measure process is simple and convenient without any harm on leaves,so SPAD readings is a useful index for selecting the high yield varieties in the early period

Impacts and mechanism of Eulaliopsis binata hedgerow system on soil and water losses control in purple hilly area in Sichuan basin.

Abstract: The purple hilly area of Sichuan basin,located in the upper reach of the Yangtze River,is one of the most severe soil erosion in China,where three quarters of its land surface are seriously affected by soil erosion and the average erosion rate can reach 3200-7900 t/km2/aThis area is the most important agricultural section of Sichuan province and Chongqing municipalExtensively cultivation,dense population and heavy soil and water losses is not only a severe environmental problem but also an obstacle to agricultural sustainable developmentTo improve soil erosion environment,reduce soil and water losses using hedgerow system is one of the most important issues in over-farming land and ecological fragile areaEulaliopsis binata(Retz)GEHubb has abundant roots and high yield,and the time when Eulaliopsis binata(Retz)GEHubb growths fast is as the same as the time of the rainy season in this area,so Eulaliopsis binata(Retz)GEhubb can mulch soil surface well and reduce soil and water losses significantlyThe total length of roots in a 120 cm wide and 90 cm deep soil profile is 987148cm,which can enlace soil about 235 circlesThe average diameter of Eulaliopsis binata(Retz)GEHubb root is from 020 mm to 050 mm,and 95% roots,diameter is thinner than 040 mmThe total surface areas of roots is 1013968 cm2,and the surface areas of roots is about 2 to 3 times to soil surface areas in the surface layerEnlacing and concretion to soil profile of Eulaliopsis binata(Retz)GEHubb root and the gigantic adsorption to soil grains of root surface are the mechanism why Eulaliopsis binata(Retz)GEHubb can reduce soil and water loss significantlyThe monitoring result from 2003 to 2004 indicates that,when the gradient is 12 degrees,the runoff rate of planting Eulaliopsis binata(Retz)GEHubb completely after changing slope land to ladder(PECC,so as the follows) is only 4990 m3/hm2·yrand the soil loss rate is 15578 kg/hm2·yr,however,the runoff rate of farming and being not change slope land to ladder(FNC,so as the follows) is 31429 m3/hm2·yr that is 630 times to PECC,and the soil loss rate is 332250 that is 2133 times to PECC;When the gradient is 24 degrees,the runoff rate of planting eulaliopsis binata(Retz)GEHubb in the ridge and border slope land after changing slope land to ladder(PERBC,so as the follows) is 24804 m3/hm2·yr and the soil loss rate is 61200 kg/hm2·yr,the runoff rate of PECC is only 4244 m3/hm2·yr and the soil loss rate is 15375 kg/hm2·yr,however,the runoff rate of FNC reaches to 78573 m3/hm2·yr that is 317 times to PERBC and 1852 times to PECC,and the soil loss rate reaches to 37503 m3/hm2·yr that is 6133 times to PERBC and 24412 times to PECC

Retrospect and prospect for the cropping system research in western plain of Sichuan

Abstract: In term of ecological and climatic conditions,this paper reviews briefly the progress of research and application of cropping system in Western Plain of Sichuan and analyzes emphatically the research background,main planting patterns and resources-utilization of the new triple cropping system since 1990s last century.Authors also put forward the future direction of cropping system in this region.

Comprehensive evaluation and screening identification indexes of cold tolerance at flowering stage in rice

Abstract: In this paper,eight morphological indexes correlating with cold tolerance including leaf age,plant height,root number,leaf area were monitored under low-temperature stress at seedling stage for 21 rice cultivars and seven physiological and biochemistry indexes correlating with cold tolerance including Chl content,soluble sugar content,soluble protein content,proline content,superoxide dismutase(SOD) activities,malondialdehyde content were monitored under low-temperature stress for 5 rice cultivars.The indexes that were significantly correlated with their cold tolerance were screened by the main content analysis and the step regression analysis.The results showed that at seedling stage leaf age and MDA content were significantly correlated with their cold tolerance and could be selected to identify the cold tolerance of rice.The cold tolerance of 5 rice cultivars was evaluated by stepwise regression equation and the results were basically consistent with the results in their livability rate,which indicated that it was feasibleto predict the cold tolerance in rice at seedling stage using the two indexes screened out in this study and the way that this paper used was feasible to comprehensive evaluate the cold tolerance in rice.

A new species and a new variety of Curvularia in China

Abstract: A new species and a new variety of Curvularia were reported.They were Curvularia sichuanensis Meng Zhang T.Y.Zhang and Curvularia verruciformis var.cucurbita Meng Zhang T.Y.Zhang.Latin diagnosis and illustration of these new taxa were provided.Type specimens were deposited in the Herbarium of Shandong Agricultural University: Plant Pathology(HSAUP).

Study on ecological adaptability and yield potential of new wheat cultivar 'Chuanmai 42' derived from synthetic hexaploid wheat.

Abstract: Wheat is one of the most important crops in the world.Increasing grain yield of wheat steadily to match the increasing demand though germplasm innovation and breeding is the great goal for agricultural scientists.Chuanmai 42 is the first new commercial cultivar of wheat with high-yielding potential derived from CIMMYT synthetic hexaploid wheat.In 2004-2006,several experiments were conducted in different regions in Sichuan province to reveal its ecological adaptability and yield potential by comparing with seven commercial wheat varieties.The results indicated that: (i) Grain yield of Chuanmai 42 ranges from 4.7 to 8.6 t/hm2,with average yield of 6.7 m2 to 7.9 t/hm2,higher than others by 7 %-15 %.The coefficient of variation in grain yield for Chuanmai 42 among five ecological experimental sites reached 20 %-25 %,relatively higher than other experimental cultivars.(ii) Grain yields of treatments with high nitrogen level(150kg/m2) were significantly higher than those of treatments with low nitrogen level(75 kg/hm2) and median to high level in basic seedlings(220-240 seedlings/m2) was beneficial to increasing grain yield.(iii) Optimum sowing date for Chuanmai 42 is from Oct.25 to Oct.30 in most regions of Sichuan province due to its strong spring.(iv) The grain filling pattern of Chuanmai 42 had a little difference from that of other cultivars and its filling rate during middle to late filling stage was high and beneficial to forming great kernels.

Determination of dimethachlon resistance in Alternaria alternata.

Abstract: To control tobacco brown spot disease rationally,reduce resistance to fungicide and reduce pesticide pollution,96 strains of Alternaria alternata were isolated from 11 region of Yunnan province and all isolates were determined their resistance to dimethachlon.The results indicated that of all tested isolates,there were 28 high resistance,29 middle resistance and 29 low resistance isolates respectively.The islates fungicide resistance from different region was different obviously,the highest resistant isolate come from Dali and Simao region and the Kunming,Honghe,Lincang isolates had lowest resistance among all tested isolates.The evident disparity of high-resistance,mid-resistance and low-resistance isolates ratio was exist at different region.AL208# from Dali Yongping was the highest dimethachlon resistant isolate,its resistance multiple arrived to 20 compared to the lowest resistance isolate AL171#,which from Hongta District of Yuxi City.The EC50 of 96 Alternaria alternata isolates was 81.34 mg/L in average,was in the range of 13.28-269.58 mg/L.

The growing status and biomass partition of oriental lilies under different light intensity.

Abstract: The growing status and biomass partition of lilies under three cultivation conditions,namely,in open land with full sunlight,in plastic tunnels with 80 % of full sunlight and under shadecloth with 30 % of full sunlight,were measured,respectively.The results showed the following.Firstly,the morphological characters,such as the plant height,the single leaf area,roots originated from stems and the special leaf area etc.were significantly affected by light intensity.Secondly,with worse light intensity stressed,lilies adjusted its biomass partition among different organs by increasing the biomass of the overground part and decreasing that of the belowground part relatively,which could be seen clearly from the increase of total dry mass of leaves and the decrease of total dry bulb mass.Thirdly,the sensitivity of different cultivars to light intensity was different.For example,Lilium 'Tiber' and Lilium 'Sorborne' were much more sensitive in the sense of morphology and biomass partition,while Lilium 'Siberia' was more stable.Fourthly,no matter what the light intensity could be,the biomass of the lilies was mainly constituted by that of leaves and that of bulbs,the former occupying 60 % of the total overground part and the latter occupying 70 %-90 % of the total belowground part.

Effects of seed tuber weight on potato as influenced by other factors under different ecological conditions in Sichuan

Abstract: study was carried out to investigate these effects and their interactions with effects of the other factors in sub-tropical double cropping systems. When evaluated at constant spacing, seed tuber weight significantly affected tuber yield in all conditions under study although seed origin, variety, altitude and season muddled these effects in varying degrees. The effects of seed tuber weight could be attributed to more main stems per plant from heavier seed tubers, resulting in more tubers produced per plant and thus per Unit area. When number of sprouts was set equal per unit area, effect of weight of seed tubers on final tuber yield per unit area was absent in seed tuber weight range from 22 to 124g. However, lighter seed tubers tended to increase leaf area per plant thus compensating the lower plant stand, with larger progeny tubers achieved. If calculated at a per stem basis the numbers of tubers produced and the tuber yields were different for the seed weight treatments; these differences were confounded by seed age effects. The number of tubers produced per unit area largely determined plant yield because the varieties used produced few tubers per plant whereas variation was caused by seed weight or seed density.

Effect of Pseudomonas spp. strain 20#-5 on Phytophthora infestans.

Abstract: Effect of different concentration of Pseudomonas spp.strain 20#-5 on the mycelial growth and germination zoospores of Phytophthora infestans indicated that pseudomonas.spp.Strain 20#-5 could inhibit the mycelial growth of Phytophthora infestans on potato,tomato and tobacco.Effect of 2.86 % fermented broth could reach higher than 60 %.High concentration of fermented broth suppressed growth of mycelial than killed them.The effect of inhibition was obvious on the germination zoospores of Phytophthora infestans.In experiment of pot culture,strain 20#-5 took a better action on protection than on control of Phytophthora infestan especially by living bacteria.

Prospect on the population carrying capacity of resource in Tibet from 1985 to 2005

Abstract: Based on analyses of the actuality of the natural,social and economical resource of Tibet,using the research theory and the calculation methods of the relative carrying capacity of synthetic resources,and selecting some data such as:population,agrarian area and GDP,the relative carrying capacity of synthetic resource of Tibet was analyzed in the past twenty years.The result showed:the contribution of the carrying capacity of natural resources to the carrying capacity of synthetic resources is much higher than that of the carrying capacity of economic resources,and the increase of the carrying capacity of synthetic resource is generally lower than the population growth;The practical carrying capacity of synthetic resource is higher than the relative carrying capacity of synthetic resource,the carrying capacity of resource of Tibet is overloaded.At the same time,the existent causes of the carrying capacity of resource of Tibet was analyzed,and the paper discuss the countermeasures of solving conflict of the man-land relationship in Tibet was discusssed.

Determination of colchiane in Iphigenia indica Kunth et Benth. by HPLC

Abstract: Simulataneous HPLC determine the colchicine in different part of bulb of Iphigenia indica Kunth et Benthand the wild population of the northwestward of Yunnan province and Yuexi county in SichuanThe result shows:The up-ground parts of Iphigenia indica Kunth et Benthcontain few colchicineThe colchicine gradually reduce from outside to inner in the bulb of Iphigenia indica Kunth et Benth,the outer-epidermis contain 0134 %,the outer of bulb contain 0171 %The content of colchicine in the twenty-two wild populations GS01 is 061 % and GS22 is 013 %The average conents of these colchicine is 040 %

Preparation and regeneration of protoplasts from Morchella sp.

Abstract: For the Morchella sp.,preparation and regeneration conditions of protoplast were reported in this paper.The results showed that the optimal liquid medium and time for the mycelial growth of two strains were No.③ liquid medium and 2-4 days,respectively,the appropriate liquid enzymes,enzymolysis time and temperature for the mycelial digesting were No.① liquid enzymes,3.5 h and 30 ℃.On the other hand,there were a lot of regeneration colony in No.③ regeneration medium after 3-5 days.

Construction and analysis of AFLP fingerprinting in Nancy Hall of sweetpotato.

Abstract: AFLP(Amplified Fragment Length Polymorphism) analysis system was established in Nancy holls that used widely sweetpotato breeding in China.These 5 pairs of primers like EATT,MCCA which had high polymorphism and powerful distinctiveness were been selected from 16 pairs of primers to establish the fingerprinting of Nancy holls.16 pairs primers were used for clustering analysis about 47 related varieties with Nancy holls.The genetic distance of Nancy holls was calculated and its dendrogram which were divided into 5 types was performed based on AFLP data of 16 pairs of primers.According to these results,the breeders could know and use Nancy holls better.

Genetic analysis of resistance to bacteria wilt in eggplant (Solanum melongena).

Abstract: The genetic parameters of resistance to bacterial wilt in eggplant were estimated by analyzing the appearance of resistance among 49 hybrids derived from the crosses between 4 resistant parents and 3 susceptible ones with complete diallel mating design.The results showed that the heredity of resistance was not correspondent with the additive-dominant model,and the resistance was controlled by a few recessive genes as well as influenced by epitasis effect.