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A manual for the practical study of root-nodule bacteria

J. M. Vincent
- Iss: 1
TLDR
A manual for the practical study of root-nodule bacteria is presented in this article, where the authors present a set of root nodule genes and root nodes.
Abstract
A manual for the practical study of root-nodule bacteria , A manual for the practical study of root-nodule bacteria , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

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Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE.

TL;DR: It was demonstrated that agricultural soil harbours a substantial diversity of nirS denitrifiers by use of the new nIRS primers, which acted as broad range primers for each of the three genes.
Journal ArticleDOI

Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal.

TL;DR: Using R. meliloti strains overproducing symbiotic Nod factors, the major alfalfa-specific signal, NodRm-1, is purified by gel permeation, ion exchange and C18 reverse-phase high performance liquid chromatography and elicited root hair deformation on the homologous host when added in nanomolar concentration.
Journal ArticleDOI

A general method for site-directed mutagenesis in prokaryotes.

TL;DR: A general technique applicable to Gram-negative prokaryotes for site-directed mutagenesis of cloned DNA fragments which is applied to the study of the symbiotic nitrogen fixation genes of Rhizobium meliloti is reported.
Journal ArticleDOI

Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

TL;DR: It is demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasid pRMESU47a, which includes four loci involved in exopolysaccharide (exo) synthesis as well as two lociinvolved in thiamine biosynthesis.
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