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All figures (8)
Figure 7. Semi-log plot of normalized scattering intensity versus radius of hydration, Rh. The solid line represents the model of a spherical particle, the dashed line represents the model of a disk, the dotted line represents the model of a rod. The dotted-square line represents the DLS data. The DLS data fit the curve for the sphere indicating the shape of the DMPC / C8E5 aggregates are spherical.
Figure 6. Rh versus theoretical q. Increasing theoretical q corresponds to an increase in the hydrodynamic radius of the particle.
Figure 2. Meff versus D2O concentration. Approximately 18.4% D2O is required to density match DMPC-C8E5 lipid-detergent aggregates.
Figure 3. Density matching using three biocompatible density modifiers: A) D2O B) glycerol C) sucrose. Approximately 71.7% (v/v) D2O, 23.5 % (v/v) glycerol and 0.418 M sucrose is required to density match DMPC-DHPC bicelles.
Figure 4. 31P-phosphorus NMR spectra of C8E5 (blue) and DMPC (red). The chemical shift of DHPC is 35.1 ppm and the chemical shift of DMPC appears at 42.0 ppm for DMPC in DMPC / DHPC bicelles and 42.5 ppm in DMPC / C8E5 bicelles. Panel A is the sample analyzed at 25 ⁰C and Panel B is the sample analyzed at 37 ⁰C. Note: Data was also collected at 31 ⁰C showing chemical shifts similar to 37 ⁰C. (spectrum not shown).
Table I. Measurements of viscosity and hydrodynamic radius in relation to increasing lipid concentration
Figure 5. Rh versus % total lipid (w/w). Particle size decreases as total lipid concentration increases.
Figure 1. A) Schematic diagram of a bicelle containing DMPC and DHPC. Samples were prepared replacing DHPC with C8E5 predicted to replace DHPC at the rim of bicelle. B) Dodecyl-β -D-maltoside (DDM) lipid with dense maltose sugar headgroup; glucose = 0.622 cm3 / g, 12-carbon chain = 0.990 cm3 / g. DDM has been used in mixed micelle systems to study the human adenosine A2A receptor expressed and isolated from S. cerevisiae.
Posted Content
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DOI
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A novel lipid-polymer system with unique properties has potential in drug delivery and biotechnology applications
[...]
Rieth
1
,
Glover Kj
2
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Institutions (2)
Southern Illinois University Edwardsville
1
,
Lehigh University
2
07 Oct 2018
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bioRxiv