Figure 4: zferv1a is expressed in cells from the lymphoid lineage in adult hematopoietic tissues. A. Single slice DIC and confocal fluorescence colocalisation image of the thymus (black star) situated underneath the ear in 5dpf embryos and the signal from the Tg(zferv1a:GFP)sh533 reporter. Scale bar 100µm. B. Cell tracking reveal dynamic behaviour of entry and exit of thymus by GFP positive cells in Tg(zferv1a:GFP)sh533. Whole stack analysis of cell movement by using TrackMate on FiJi. Scale bar 10µm. C. Expression of Tg(zferv1a:GFP)sh533 in a 25dpf zebrafish highlighting the triangular shaped thymus (white arrowhead). D. Side scatter (SSC) and GFP plot from single cell suspension of a whole kidney marrow from an adult Tg(zferv1a:GFP) with gate selecting GFP positive cells. Inset plot showing the distribution of cells from a non-transgenic adult whole kidney marrow. E. Forward and side scatter (FSC/SSC) plot of selected GFP positive cells from panel D. Inset plot showing the location of the GFP positive cells in the haematopoietic lineage FSC/SSC plot. F. FSC/SSC plot of a whole kidney marrow separating different haematopoietic lineages by size and granularity.
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