Patent
Electrochemical sensor having three layer membrane containing immobilized enzymes
Yasusi Niiyama,Kenshi Sugahara +1 more
TLDR
In this article, an electrochemical sensor is formed having a working electrode for detecting hydrogen peroxide surrounded by a cylinder portion, and with an enzymecontaining membrane at its tip.Abstract:
An electrochemical sensor is formed having a working electrode for detecting hydrogen peroxide surrounded by a cylinder portion, and with an enzymecontaining membrane at its tip. The membrane has a porous layer permeable to hydrogen peroxide between a layer containing an immobilized enzyme capable of decomposing hydrogen peroxide and a layer containing an immobilized enzyme capable of decomposing a substrate to form hydrogen peroxide. The cylinder portion is embedded in the layer containing the hydrogen peroxide decomposing enzyme and surrounds the working electrode such that the electrode is in contact with the porous layer but is not in contact with the layer containing the hydrogen peroxide decomposing enzyme. The layer containing the hydrogen peroxide forming enzyme is on a side of the porous layer opposite the electrode so as not to contact the electrode. Activity of the hydrogen peroxide decomposing enzyme is no more than one-fourth of the activity of the hydrogen peroxide forming enzyme. The electrochemical sensor reduces base line elevation after measurement action has been discontinued and measurement of a next sample is restarted.read more
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Patent
Analyte Monitoring Device And Methods Of Use
James Say,Michael F. Tomasco,Adam Heller,Yoram Gal,Behrad Aria,Ephraim Heller,Phillip John Plante,Mark S. Vreeke,Keith A. Friedman,Fredric C. Colman +9 more
TL;DR: An analyte monitor includes a sensor, a sensor control unit, and a display unit as discussed by the authors, which is used to display an indication of a level of an analyte, based on the data obtained using the sensor.
Patent
Subcutaneous glucose electrode
Adam Heller,Michael V. Pishko +1 more
TL;DR: In this article, a small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described, which is designed to allow "one-point" in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood.
Patent
Small volume in vitro analyte sensor
TL;DR: In this article, a sensor is designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator.
Patent
Electrochemical analyte sensor
James Say,Michael F. Tomasco,Adam Heller,Yoram Gal,Behrad Aria,Ephraim Heller,Phillip John Plante,Mark S. Vreeke +7 more
TL;DR: An electrochemical analyte sensor formed using conductive traces on a substrate can be used for determining and/or monitoring a level of analyte in in vitro or in vivo analyte-containing fluids as discussed by the authors.
Patent
Method of using a small volume in vitro analyte sensor
TL;DR: In this article, a sensor is designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator.
References
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Patent
Surface-modified electrodes
TL;DR: In this paper, a surface-modified electrodes which may be used in electrochemical cells for production of electrical energy comprise an enzyme immobilized on a support consisting of at least a monolayer coating of a carbonaceous pyropolymer possessing recurring units containing at least carbon and hydrogen atoms composited on a high surface area refractory inorganic oxide.
Patent
Enzyme electrode provided with immobilized enzyme membrane
TL;DR: An enzyme electrode comprising an anode, a cathode, an immobilized enzyme membrane, and an electrolyte is used for polarographic determination of a small amount of components in body fluid, body tissue, foodstuffs, or the like without any undesirable hindrance with low molecular interfering materials without using any other specific means for removing such low molecular interferences as mentioned in this paper.
Patent
Electrochemical sensor having an immobilized enzyme membrane
TL;DR: In this article, a polyester woven fabric on which urease is immobilized is attached to the vessel so as to cover the NH 4 + selective membrane, and the immobilized enzyme membrane is applied with an appropriate tension by the slightly protruded ion selective membrane.
Patent
Immobilized enzyme membrane
TL;DR: In this article, an asymmetric membrane for using at the working face of an electrochemical electrode is prepared which comprises an asymmetrical membrane integrally formed from a skin layer substantially incapable of permeating an enzyme therethrough but capable of permeATING a gas and a liquid, and a sponge layer having pores containing an enzyme.
Patent
Glucose oxidation with immobilized glucose oxidase-catalase
TL;DR: In this article, an immobilized glucose oxidase-catalase composition was used for economic oxidation of glucose in a reaction mixture at low temperatures below 15°C, and the freezing point of the reaction mixture between 10° C and 15° C.