Journal ArticleDOI
Growth and metabolic activities of heat treated Staphylococcus aureus.
M. C. Allwood,A. D. Russell +1 more
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Variation in the composition of the metabolic pool of heated cells occurred during the early lag phase and may be as a result of damage to the cytoplasmic membrane with resulting loss of permeability control.Abstract:
Staphylococcus aureus cells which had been heated at 50 or 60° were transferred to various growth media and intracellular ribonucleic acid (RNA), deoxyribonucleic acid (DNA) and amino acids were measured during the lag phase of growth. The duration of the lag phase depended on the temperature to which the cultures had been subjected, and was longest following storage at 60°. RNA synthesis occurred almost immediately on placing treated cells in a growth medium, but at a slower rate than with unheated cells. Variation in the composition of the metabolic pool of heated cells occurred during the early lag phase and may be as a result of damage to the cytoplasmic membrane with resulting loss of permeability control.read more
Citations
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Journal ArticleDOI
Lethal effects of heat on bacterial physiology and structure.
TL;DR: High temperatures have profound effects on the structural and physiological properties of sporulating and non-sporulating bacteria, with membranes, RNA, DNA, ribosomes, protein and enzymes all affected.
Book ChapterDOI
Mechanisms of Thermal Injury in Nonsporulating Bacteria
M.C. Allwood,A.D. Russell +1 more
TL;DR: Although moist and dry heat have been used as methods of sterilization for a considerable period of time, it is not unreasonable to propose that this effect masks other, more delicate, changes in the bacterial cell that could be induced before coagulation becomes apparent.
Journal ArticleDOI
Repair of injury in freeze-dried Salmonella anatum.
B. Ray,J.J. Jezeski,F. F. Busta +2 more
TL;DR: In this article, the authors showed that repair of freeze-drying Salmonella anatum in nonfat milk solids occurred rapidly after rehydration, independent of influence of pH between 6.0 and 7.0.
Book ChapterDOI
Chapter V Methods for Assessing Damage to Bacteria Induced by Chemical and Physical Agents
TL;DR: This chapter presents various methods of studying the effects of antibiotics and other chemotherapeutic agents and of heat, ionizing, and ultraviolet radiations on bacteria at the cellular, biochemical, and molecular levels.
Journal ArticleDOI
Thermal injury of yersinia enterocolitica
L Restaino,W S Jeter,W M Hill +2 more
TL;DR: The use of metabolic inhibitors demonstrated that ribonucleic acid synthesis was required for repair, whereas deoxyribon nucleic, cell wall, and protein synthesis were not necessary for recovery of 0:17 cells injured in 0.1 M PO4 buffer, BHI, or PI.
References
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Journal ArticleDOI
Repair of Thermal Injury of Staphylococcus aureus
John J. Iandolo,Z. John Ordal +1 more
TL;DR: The data presented demonstrate that thermal injury, in part, occurred owing to changes in the cell membrane, which allowed soluble cellular components to leak into the heating menstruum, and implied that ribonucleic acid synthesis was particularly involved.
Journal ArticleDOI
Effects of thermal stress on viability and ribonucleic acid of aerobacter aerogenes in aqueous suspension.
R. E. Strange,M. Shon +1 more
TL;DR: Generally, conditions which accelerated the death-rate of Aerobacter aerogenes at 47° also increased the rate of degradation of endogenous RNA and this was accompanied by an increase in the ultraviolet absorption of cold acid-extracts of bacteria and of the suspending fluid.
Journal ArticleDOI
Regeneration of Ribosomes and Ribosomal Ribonucleic Acid During Repair of Thermal Injury to Staphylococcus aureus
Stephen J. Sogin,Z. John Ordai +1 more
TL;DR: The data presented here further substantiate the noninvolvement of protein synthesis during recovery and further demonstrate the site of the thermally induced nucleic acid lesion.
Journal ArticleDOI
The Effect of Sublethal Heat Treatment on the Growth of Staphylococcus aureus.
H. Jackson,M. Woodbine +1 more
TL;DR: When an enterotoxigenic strain of Staphylococcus aureus was subjected to sublethal heat treatment and subsequently inoculated into nutrient broth and incubated at 37° there was a fall in viable numbers, followed by a lag phase of growth.
Journal ArticleDOI
Thermally Induced Ribonucleic Acid Degradation and Leakage of Substances from the Metabolic Pool in Staphylococcus aureus
M. C. Allwood,A. D. Russell +1 more
TL;DR: There is a leakage of free amino acids, protein, and 260 mmu-absorbing material from both types of cell suspension, and membrane damage, as measured by the intracellular penetration of 8-anilino-1-naphthalene-sulfonic acid, may be partially related to this leakage.
Related Papers (5)
Regeneration of Ribosomes and Ribosomal Ribonucleic Acid During Repair of Thermal Injury to Staphylococcus aureus
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