Journal ArticleDOI
Lactate dehydrogenase and malate dehydrogenase isozyme patterns in tissues of temperature-acclimated goldfish (Carassius auratus L.).
TLDR
Electrophoretic analyses reveal no detectable qualitative or quantitative alterations in the isozyme patterns due to the effects of temperature acclimation at 5, 15 and 25°C.Abstract:
1. 1. The lactate dehydrogenase and malate dehydrogenase isozyme patterns from goldfish ( Carassius auratus L.) tissues were examined by starch gel electrophoresis and immunoprecipitation. 2. 2. Electrophoretic analyses reveal no detectable qualitative or quantitative alterations in the isozyme patterns due to the effects of temperature acclimation at 5, 15 and 25°C. 3. 3. The only variations of LDH * isozyme patterns observed were those attributable to genetic polymorphism and to different ratios of red and white skeletal muscle fibres.read more
Citations
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Journal ArticleDOI
Evolution of a gene. Multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation
Journal ArticleDOI
Temperature Adaptation of Enzymes: Biological Optimization Through Structure-Function Compromises
TL;DR: Most aspects of enzyme structure and function are highly sensitive to temperature variation, whether this variation occurs rapidly or over long evolutionary time, and the so-called "goals" of temperature adaptation are identified.
Journal ArticleDOI
Specialized lactate dehydrogenase isozymes: The molecular and genetic basis for the unique eye and liver LDHS of teleost fishes
TL;DR: Electrophoretic and immunochemical analysis of the lactate dehydrogenase isozyme patterns of fishes indicates that these isozymes are the tetrameric products of three LDH loci — A, B, and C — which are present in virtually all teleost fishes.
Book ChapterDOI
Molecular and cytological investigations
TL;DR: Molecular and cytogenetic studies of cyprinid fishes continue to contribute to the understanding of this diverse and fascinating group and have addressed questions regarding historical evolution, i.e. phylogenetic relationships and, to a lesser extent, zoogeography.
References
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Journal ArticleDOI
Starch gel electrophoresis of enzymes—A compilation of recipes
Charles R. Shaw,Rupi Prasad +1 more
TL;DR: Most of the methods outlined here have been used primarily for tissue extracts from mammalian species, and a few have been developed only on plants or lower animals, but this is not to say that they will not work on higher organisms.
Journal ArticleDOI
Lactate dehydrogenase isozyme patterns of fish.
TL;DR: Fish may be classified into four groups on the basis of their possession of one, two, three, or five major isozymes of LDH, which can be attributed to the polymers of two protein subunits under the control of two genes, as in mammals and birds.
Journal ArticleDOI
Developmental genetics of the lactate dehydrogenase isozymes of fish
TL;DR: Three genes (A, B, and E) encoding lactate dehydrogenase (LDH) subunits are shown to be present in fish by: (1) molecular hybridization, (2) allelic variants at two loci, and (3) kinetic, physical, and immunochemical studies.
Journal ArticleDOI
Functional significance of isoenzymes in thermal acclimatization. Acetylcholinesterase from trout brain
John Baldwin,Peter W. Hochachka +1 more
TL;DR: It is concluded that the K(m)-temperature relationship is adaptive, and that the critical process during thermal acclimatization, in cases where enzymes show sharp changes in K( m) with temperature, is the synthesis of a new enzyme variant that is better suited for catalysis and control of catalysis under the conditions of theacclimatized state.
Journal ArticleDOI
The effect of temperature acclimation on pathways of glucose metabolism in the trout
P. W. Hochachka,F. R. Hayes +1 more
TL;DR: In warm (15 °C) acclimated Salvelinus fontinalis, the respiration of epaxial muscle homogenates was almost completely inhibited by iodoacetate and C14O2 was incorporated primarily into positi...
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Functional significance of isoenzymes in thermal acclimatization. Acetylcholinesterase from trout brain
John Baldwin,Peter W. Hochachka +1 more