Leukocyte antigens in renal transplantation. IV. The effect of blood transfusions on leukocyte typing by lymphocytotoxicity.

TLDR: No change in the results of typing were found, even within 5 min of transfusion, in 22 of these 25 studies, providing a strong argument in favour of the use of a cytotoxic technique for leukocyte typing in renal transplantation, where recipients and cadaver donors often have received blood transfusions before typing.

Abstract: The reproducibility of leukocyte typing by a microdroplet lymphocytotoxicity test was studied in 25 patients after blood transfusions of 1.5–6 litres. No change in the results of typing were found, even within 5 min of transfusion, in 22 of these 25 studies. This contrasts markedly with typing by leukoagglutination where prior transfusions can significantly falsify results. This provides a strong argument in favour of the use of a cytotoxic technique for leukocyte typing in renal transplantation, where recipients and cadaver donors often have received blood transfusions before typing.