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Patent

Method for quantitatively determining a particular substrate catalyzed by a multisubstrate enzyme

Jay M. Johnson
TLDR
In this article, a multisubstrate enzyme was incorporated into a thin layer electrochemical cell laminate having exterior membrane layers and an interior enzyme layer, and a control electrode located within the enzyme layer applied an electrical potential to the enzyme.
Abstract: 
A method for control of relative enzyme activity for analytical purposes. The activity of a multisubstrate enzyme may be controlled as a function of the electrical potential applied to the enzyme. The enzyme is preferrably incorporated into a thin layer electrochemical cell laminate having exterior membrane layers and an interior enzyme layer. A control electrode located within the enzyme layer applies an electrical potential to the enzyme. An intermediate electron transfer agent may be used to transfer electrons to and from the enzyme and the control electrode.

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Patent

Analyte Monitoring Device And Methods Of Use

James Say, +9 more
TL;DR: An analyte monitor includes a sensor, a sensor control unit, and a display unit as discussed by the authors, which is used to display an indication of a level of an analyte, based on the data obtained using the sensor.
Patent

Subcutaneous glucose electrode

Adam Heller, +1 more
TL;DR: In this article, a small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described, which is designed to allow "one-point" in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood.
Patent

Small volume in vitro analyte sensor

Adam Heller, +3 more
TL;DR: In this article, a sensor is designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator.
Patent

Electrochemical analyte sensor

James Say, +7 more
TL;DR: An electrochemical analyte sensor formed using conductive traces on a substrate can be used for determining and/or monitoring a level of analyte in in vitro or in vivo analyte-containing fluids as discussed by the authors.
Patent

Method of using a small volume in vitro analyte sensor

Adam Heller, +3 more
TL;DR: In this article, a sensor is designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator.
References
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Patent

Membrane for enzyme electrodes

David P. Newman
TL;DR: A laminated membrane for use with an enzyme electrode without the need for use of a compensating electrode was proposed in this paper, which consists of essentially homogeneous material such as cellulose acetate or silicone rubber which will prevent passage of even low molecular weight interfering materials, an adhesive layer of the enzyme itself (with or without other materials that may be blended with it), and a layer of support film which will also prevent the passage of high molecular weight interference materials.
Patent

Method and apparatus for continuously controlling an enzymatic reaction

Jean Marie Fresnel, +1 more
TL;DR: In this article, a control method and apparatus for carrying out enzymatic reactions at a constant rate is described. But this method requires the enzyme to be fixed on a solid electronically conductive support.
Patent

Method and apparatus for governing the reaction rate of enzymatic reactions

Jean-Marie Fresnel, +1 more
TL;DR: In this article, the potential of the enzyme support is adjusted by adjusting the mean value of the potential to maintain a corresponding mean enzymatic activity for transforming the substrate at a desired rate.
Patent

Stabilization of activated galactose oxidase enzyme

Jay M. Johnson
TL;DR: Galactose oxidase enzyme can effectively be used for the determination of a number of different substances if activated and stabilized in accordance with the present invention as discussed by the authors, but it is not suitable for the use in medical applications.
Patent

Method for measuring substrate concentrations

Philippe Racine, +3 more
TL;DR: In this article, the concentration of substrates of enzyme reactions is measured by determining the current produced at an platinum or gold electrode by oxidizing an acceptor utilizing a measuring device comprising a measuring cell, an enzyme electrode in contact with the fluid sample and containing a reference electrode.