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Open AccessJournal ArticleDOI

Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan

Endang Gati Lestari
- Vol. 7, Iss: 1, pp 63-68
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TLDR
In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction and the existence of a certain growth regulating substances can enhance growth regulator activity of other substances.
Abstract
The Role of Growth Regulator in Tissue Culture Plant Propagation. Endang G. Lestari. In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction. Cytokinin and auxin are two prominent growth regulator. Cytokinin consists of BA (benzil adenin), kinetin (furfuril amino purin), 2-Ip (dimethyl allyl amino purin), and zeatin. While auksin covers IAA (indone acetic acid), NAA (napthalene acetic acid), IBA (indole butiric acid) 2.4-D (2.4- dicholophenoxy acetic acid), dicamba (3,6 dicloro-O-anisic acid), and picloram (4-amino 3,5,6-tricloropicolinic acid). The emphasis of plant growth purposes decide the use of growth regulator. Cytokinin is applied mainly for the purpose of shoot, while auxin is mainly used for the purpose of root and callus. The application of growth regulator application is varied, depending on the genotype and physiological condition of the plant. The existence of a certain growth regulating substances can enhance growth regulator activity of other substances. The type and concentration of the appropriate growth regulators for each plant is not the same because it depends on the genotype and physiological condition of plant tissue. However so often both are frequently required depend on the ratio/ratio of auxin cytokines or vice versa. The existence of a certain growth regulating substances can enhance growth regulator activity of other substances. The type and concentration of the appropriate growth regulators for each plant is not the same because it depends on the genotype and physiological condition of plant tissue. For the propagation, multiple and adventive shoots along with embriosomatic formation could be applied. The seedling is obtained from one somatic cell. Here, strong auxin, such as dicamba and picloram 2.4-D, is utilized for callus production. For this reason, seedling per unit could be produced more than that of organogenesis.

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Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin

TL;DR: The results showed that explants could not produce callus, the explants response just a swelling leaf explants, and the treatments that could maintain 66.67% explants growth was the combination 0.4-D + 0.5 mg / L 2.5mg / L kinetin.
Dissertation

Pengaruh penambahan Zat Pengatur Tumbuh (ZPT) terhadap perkecambahan dan induksi kalus embrionik tanaman cendana (Santalum album L.) secara in vitro

TL;DR: Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL), perkecambahan biji cendana mengg unakan IBA dan BAP, anda pengaruh 2,4-D terhadap induksi kalus embrionik dari daun dan kotiledon c endana (Santalum album L.) secara in vitro.
Journal ArticleDOI

Micropropagation of Red Ginger (Zingiber officinale Rosc. Var. Rubrum) Using Several Types of Cytokinins

TL;DR: This study aimed to obtain the best type and concentration of cytokinins in increasing the multiplication of red ginger shoots in vitro and showed that 1 ppm thidiazuron treatment produced the highest number of shoots and the highest shoot length in the first subculture.
Journal ArticleDOI

PENGARUH ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS DAN BAHAN PENYANGGA PADA PEMBENTUKAN PLANTLET KANTONG SEMAR ADRIANII (Nepenthes adrianii) DENGAN KULTUR IN VITRO

TL;DR: Tahap Multiplikasi Tunas menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor, dan tujuan penelitian pada tahap pembentukan plantlet adalah untuk mendapatkan tanaman kecil yang sejati.
Journal ArticleDOI

In vitro embryogenic callus induction of Carica pubescens Lenne and K.Koch using 2,4-D (2,4-Dichlorophenoxy Acetic Acid) and BAP (6-Benzylaminopurin):

TL;DR: In this article, the effect of 2,4-D growth regulators and BAP interactions on the induction of embryogenic callus C. pubescens was investigated using a completely randomized design with 25 treatments and 5 replications.