Patent
Reaction device and method for visual detection of isothermal amplification of nucleic acid
Wu Jian,Zhang Fang +1 more
TLDR
In this paper, a method and a reaction device for visual detection of isothermal amplification of nucleic acid is presented, which comprises the following steps: in a special hermetic reaction device, adding a reagent capable of decomposing pyrophosphoric acid into phosphoric acid, after reaction, under the condition of not uncapping, mixing the amplified reaction liquid with other selected color reagents which are additionally stored in the device in advance to react and observing the color of the mixture after further reaction.Abstract:
The invention discloses a method and a reaction device for visual detection of isothermal amplification of nucleic acid. The method comprises the following steps: in a special hermetic reaction device, adding a reagent capable of decomposing pyrophosphoric acid into phosphoric acid into a nucleic acid amplified reaction liquid in advance; after reaction, under the condition of not uncapping, mixing the nucleic acid amplified reaction liquid with other selected color reagents which are additionally stored in the device in advance to react and observing the color of the mixture after further reaction; and judging whether nucleic acid amplified reaction is carried out on a sample or not, wherein the selected color reagents are reagent combination capable of being in color reaction with phosphoric acid (radicals). The method disclosed by the invention is simple to operate and is quick and sensitive. The test result can be detected by virtue of naked eyes. The method is low in cost and makes up the defects in the existing technology for detecting nucleic acid amplification.read more
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Patent
Sealed portable nucleic acid detection apparatus and method thereof
TL;DR: In this article, a portable visualized nucleic acid detection method and a sealed portable NCA detection apparatus are presented, where a visualized mixing container is provided with a plurality of connection ports, such that the visualised mixing container can be used as the total sealing body for connecting the NCA reaction tube and the detection reaction liquid storage container.
Patent
Detection method for nucleic acid amplification
Jian Wu,Liu Wang,Ying Yibin +2 more
TL;DR: In this article, a detection method for nucleic acid amplification is proposed, which comprises the following steps: adding a reaction amplification solution in a first PCR pipe, then adding calcein and a MnO2 nano-material into a second PCR pipe and adding a pyrophosphate solution into a third PCR pipe; respectively placing test strip probes into the second and third PCR pipes, or adding an antigen-modified primer into the first and the third PCR pipelines, respectively.
Patent
Vibrio parahaemolyticus detection kit and detection method thereof
Xu Wentao,Lyu Shuxia,Luo Yunbo,Huang Kunlun,Li Xuetong,Su Yuan,Xiaoyun He,Du Zaihui,Zhu Longjiao +8 more
TL;DR: In this paper, the authors proposed a method for the detection of vibrio parahaemolyticus, which combines a single cross-primer constant-temperature amplification technology and amolybdenum-rhenium anti-photometric method.
Patent
Integrated tubular reaction device
Su Xing,Kai Yuan Wu +1 more
TL;DR: In this paper, an integrated tubular reaction device consisting of a reaction vessel, a cover body and a seal member is proposed to resolve the problem of reaction product pollution in processes of multiple or multi-step biological enzyme reactions.
References
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Patent
Container and method for detecting nucleic acid reaction
TL;DR: In this paper, the authors proposed a container for detecting nucleic acid reaction, which has the advantages of simple and convenient operation and low cost, and a method for detecting the nucleic acids reaction by using the container.
Patent
Visual detection method of isothermal amplification products of nucleic acids
TL;DR: In this paper, two visual detection reagents of isothermal amplification products of nucleic acids were used, and the two reagents are used alone, at the beginning of a reaction, the reagent 1 or loses the magnesium ions to become blue from red, and reagent 2 becomes yellow from orange, amplification reaction results can be judged by color change, detection without opening of a cover can be realized by the method, the aerosol pollution can be overcome and the false positive problem caused by primer dimmers can be solved.
Patent
Method for quantification of total phosphorous
TL;DR: In this paper, a method for quantifying the total phosphorus in water of interest by decomposing phosphorous compounds contained in the water to convert the phosphorous compound into phosphate ions and determining the quantity of the phosphate ions in water, wherein an alkali metal salt of peroxydisulfuric acid and sulfuric acid are added to the water and the resulting mixture is heated at a temperature ranging from 65 DEG C to a boiling point for a predetermined period.
Related Papers (5)
Reaction device for visual detection of nucleic acid isothermal amplification
Wu Jian,Zhang Fang +1 more