Journal ArticleDOI
Sectioning of undecalcified bone; with special reference to radioautographic applications.
TLDR
A rapid technic for the preparation of 6 μ serial sections of undecalcified bone is described and microscopic structural detail is preserved and there is no evidence of translocation of the radioactivity.Abstract:
A rapid technic for the preparation of 6 μ serial sections of undecalcified bone is described. The specimen is fixed and dehydrated in acetone and ether. It is then treated with a 1:1 mixture of the monomers of ethyl and n-butyl methacrylate catalyzed with benzoyl peroxide. The monomers are removed with ether and the ether is removed under vacuum. Infiltration is accomplished under vacuum using a partial polymer of the same mixture of monomers. Polymerization is completed in 36 hours under pressure at 50°C. The tissue is sectioned with a heavy-duty microtome, the sections are mounted on glass slides and nuclear emulsions applied. Young and adult bone of laboratory animals and man have been cut successfully. Microscopic structural detail is preserved and there is no evidence of translocation of the radioactivity.read more
Citations
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Journal ArticleDOI
Preparation of thin undecalcified bone sections by rapid manual method.
TL;DR: Sections from 3 μ to over 100 μ thick of fresh, unfixed, unembedded, unde-calcified and undehydrated bone are made by grinding 1 to 2 mm slabs of the desired orientation on waterproof carborundum abrasive paper, grit No. 320, 360 or 400.
Journal ArticleDOI
Präparative Voraussetzungen zur klinischen Histologie des menschlichen Knochenmarks
TL;DR: In this article, a neue arbeitstechnik zur lichtmikroskopischen Acrylat-Histologie wird beschrieben, eignet sich besonders für die routinemasige Verarbeitung von Knochenmarksbiopsien, deren diagnostischer und wissenschaftlicher Wert bisher zu wenig erschlossen war.
Journal ArticleDOI
Preparatory conditions for clinical histology of human bone marrow. 2. A new procedure for histological preparation of bone and bone marrow biopsies
Journal ArticleDOI
An improved method for embedding hard tissue in polymethyl methacrylate.
Rob Buijs,Arend A. Dogterom +1 more
TL;DR: An improved routine method for embedding tissue, especially hard tissue, in polymethyl methacrylate (pMMA) is described and bis (4-tert-butylcyclohexyl)peroxydicarbonate (bbpd) is preferred to bpo because it is not explosive, far less is needed and it has a suitable half life.
Journal ArticleDOI
A Method for the Preparation of Undecalcified Bone Sections for Light Microscopy and Microradiography
TL;DR: A method which gives good quality 1-2 μm thick sections of undecaldfied cancellous and thin cortical bones for light miuoscopy is described and the same specimens can be used to prepare thick sections for microradiography.
References
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Journal ArticleDOI
Calcification and ossification. Calcification in normal growing bone
Franklin C. McLean,William Bloom +1 more
Journal Article
Radioautography; theory, technic, and applications.
Journal ArticleDOI
Embedding and sectioning undecalcified bone, and its application to radioautography.
TL;DR: A method is described for embedding and sectioning hard, undecalcified bone, which is designed for use by technical personnel and the results obtained are illustrated.