Pathogenic e.coli in animal feed?5 answersPathogenic E. coli has been found in animal feed. A study compared different products for pathogen control in animal feed and found that a novel non-formaldehyde combination product, as well as certain organic acid feed additive products, significantly reduced Salmonella counts in poultry feed. Another study investigated the prevalence of E. coli contamination and E. coli virulence gene signatures in commercially available conventional diets and raw-meat-based diets (RMBDs). They found that RMBDs had bacterial contamination, including E. coli, and identified different pathotypes of E. coli in these diets. Additionally, E. coli populations in swine production systems can acquire virulence and antibiotic resistance genes, leading to disease-causing strains that are difficult to manage with antibiotics. Furthermore, E. coli strains with virulence-associated genes have been isolated from feed and ingredients, highlighting the need for strict microbiological control during food production. The use of antimicrobials in food-producing animals contributes to the development and spread of antimicrobial resistance in E. coli, which can be transferred through the food chain.
What is the role of the flich7 gene in E. coli virulence and pathogenesis in broiler?5 answersThe fliC gene plays a role in the virulence and pathogenesis of E. coli in broiler chickens. It is involved in the regulation of global gene expression and signal transduction pathways, enabling the survival of E. coli under various environmental conditions. The fliC gene is part of the FlhD/FlhC control system, which transduces signals from the environment to several cellular processes. In E. coli O157:H7, a parent strain compared to its isogenic flhC mutant, the expression pattern of the fliC gene was found to be associated with central metabolism, cell division, biofilm formation, and pathogenicity. The flhC mutant exhibited higher expression levels of genes related to these cellular functions, indicating that the fliC gene is involved in regulating these processes. Overall, the fliC gene contributes to the complex regulatory network of E. coli, allowing it to survive and cause disease in broiler chickens.
What infected dose of E.coli in broiler?4 answersThe minimal oral colonization dosage of E. coli in broiler chickens was found to be 101 colony forming units (cfu). In another study, the infective dose-50 of E. coli isolates was determined to be 2 X 10^2.6 bacterial cells per ml for one isolate and 2 X 10^1.8 bacterial cells per ml for another isolate.
Isolation of e coli5 answersIsolation of E. coli can be achieved through various methods. One study conducted a retrospective analysis of human clinical specimens and found that 5.09% of the total specimens were positive for E. coli growth. Another study focused on isolating E. coli from water samples and identified biofilm-producing strains in drinking water. Additionally, a study aimed at developing alternative approaches to combatting bacterial infections successfully isolated bacteriophages from environmental objects that were capable of lysing clinical strains of E. coli. These bacteriophages showed high antibacterial activity against pathogenic E. coli strains. Overall, these studies highlight the importance of isolating and characterizing E. coli strains for various purposes, such as understanding antibiotic resistance, studying virulence genes, and developing alternative therapies for bacterial infections.
Does the presence of E. coli in poultry challenged with salmonella affect the growth of the bacteria?5 answersThe presence of E. coli in poultry challenged with salmonella can affect the growth of the bacteria. In a study by Campos et al., it was found that E. tenella infection in chickens led to a significant differential abundance of bacterial taxa, including an increase in Escherichia coli in the cecal contents and mucosal microbiota. Additionally, another study by Wang et al. showed that the addition of antimicrobial peptide microcin J25 (MccJ25) decreased the population of E. coli in the feces of broiler chickens. These findings suggest that the presence of E. coli can be influenced by factors such as infection and the use of antimicrobial peptides. However, further research is needed to fully understand the relationship between E. coli and salmonella growth in poultry.
How does Escherichia coli affect the health of chickens?5 answersEscherichia coli (E. coli) can have a significant impact on the health of chickens. It is a common bacterium found in the intestinal tract of birds and can cause a disease called colibacillosis, which is considered one of the most common and important diseases in poultry. Colibacillosis can affect birds of all ages and different types of poultry production, including broiler chickens, commercial layers, and breeders. The ability of E. coli to cause colibacillosis varies, and it can act as a primary or secondary pathogen depending on various predisposing factors. Antibiotics have been used as the main treatment for colibacillosis, but the emergence of antibiotic resistance has highlighted the need for alternative treatments and preventive measures. Probiotics have shown promise in improving the health of chickens and reducing E. coli infections, but further research is needed to develop effective strategies. The presence of antibiotic-resistant E. coli serotypes in chickens poses a potential threat to the poultry industry and public health.