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Amylase, alkaline protease, and laccase activity within biogas slurry 


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Amylase, protease, and laccase activities have been studied in biogas slurry. The isolation of a strain yielding both amylase and protease in the early stage of biogas slurry has been reported, with amylase and protease activities of 102.61U/mL and 92.93U/mL, respectively . Additionally, a multi-enzymatic biopreparation from Trichoderma atroviride G79/11 origin exhibited amylase and protease activities among other enzymes, enhancing biogas production by 30% when applied to organic waste prior to fermentation . Furthermore, the accumulation of amylase and protease through anaerobic co-digestion of waste-activated sludge and food waste was investigated, showing optimal temperatures for amylase and protease activities at 37°C and 50°C, respectively . These studies collectively highlight the presence and significance of amylase and protease activities in biogas slurry, contributing to enhanced biogas production.

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The study focused on proteolytic and lipolytic activities; amylase activity was observed in the tested strains isolated from sewage sludge, enhancing biogas production. Alkaline protease and laccase activities were not specifically mentioned.
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