scispace - formally typeset
Search or ask a question

Can CRISPR be used to diagnose COVID-19? 

Pandemic
Guide RNA
CRISPR
Cas9
Subgenomic mRNA
Best insight from top research papers

CRISPR-based diagnostic techniques have shown promise in detecting and diagnosing COVID-19 . These techniques offer rapid and accurate assays for the detection of the SARS-CoV-2 virus . Different CRISPR-based methods have been developed, including the use of dCas9-sgRNA complexes to specifically detect viral genes . The CRISPR/Cas12 system has been employed in point-of-care tests, showing high sensitivity and rapid detection of the virus . Additionally, CRISPR-based amplification-free viral RNA electrical detection platforms have been developed for the rapid identification of SARS-CoV-2 variants . These platforms utilize CRISPR RNA assays to discriminate between mutant and wild RNA genomes, achieving highly sensitive detection of single-base mutations . Overall, CRISPR technology has proven to be effective, specific, and sensitive in diagnosing COVID-19, making it a promising tool for disease control and management .

Answers from top 5 papers

More filters
Papers (5)Insight
Journal Article•DOI
CRISPR-Mediated Profiling of Viral RNA at Single Nucleotide Resolution.
Duo Chen, Wan Qi Huang, Yun Zhang, Bowen Chen, Jie Tan, Yanbing Yang, Quan Yuan 
22 May 2023-Angewandte Chemie
Yes, CRISPR can be used to diagnose COVID-19. The paper describes a CRISPR-based platform called CAVRED that enables rapid detection and identification of SARS-CoV-2 variants.
Open access•Journal Article•DOI
CRISPR as a novel technique for COVID-19 diagnosis: a review
Aisha Saleem, Aaedah F Annooz, Milane A. Hadi, Arkan Hammoodi Hasan Kabla 
26 Apr 2023-Ukrainian Biochemical Journal
Yes, CRISPR technology has been proven to be effective in detecting COVID-19 due to its simplicity, specificity, and high sensitivity.
Journal Article•DOI
CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution
Dragana Šunjka, Claire Dickinson 
16 Jun 2023-Angewandte Chemie
Yes, CRISPR can be used to diagnose COVID-19. The paper describes a CRISPR-based platform called CAVRED that can rapidly detect and identify SARS-CoV-2 variants without the need for amplification.
Open access•Journal Article•DOI
Rapid Detection of SARS-CoV-2 Based on the LAMP Assay Associated with the CRISPRCas12a System
Karoline Almeida Felix de Sousa, Carolina Kymie Vasques Nonaka, Ricardo Khouri, Clarissa Araújo Gurgel Rocha, Carlos Gustavo Regis-Silva, Bruno Solano de Freitas Souza 
30 Jun 2023-Diagnostics
Yes, the paper reports that the CRISPR/Cas12a system can be used as a diagnostic tool for COVID-19.
Open access•Journal Article•DOI
A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2
Mustapha Aouida, Maryam Saifaldeen, Dana E Al-Ansari, Sara Taleb, Ali Ait Hssain, Dindial Ramotar 
14 Jun 2023-Frontiers in Molecular Biosciences
Yes, CRISPR can be used to diagnose COVID-19. The paper describes a CRISPR-based approach using dead Cas9-sgRNA to detect the SARS-CoV-2 virus.

Related Questions

What are the current uses of crispr?5 answersCRISPR technology has a wide range of current uses. It has transformed multiple fields, including cancer and immunology, by enabling direct genomic manipulation of immune cells for unbiased functional genetic screens and the discovery of novel drug targets. CRISPR-Cas systems are being used as therapeutics for human diseases, including monogenic diseases like sickle cell anemia and complex heterogenous diseases like cancer and diabetes. In ophthalmology research, CRISPR/Cas9 has been used to correct pathogenic mutations in eye stem cells and has shown promise in treating inherited retinal disorders. CRISPR-Cas13, a type VI CRISPR-Cas system, has been adapted for various applications, including killing antibiotic-resistant bacteria, developing highly specific detection tools, regulating gene expression, and modifying RNA in vivo. CRISPR/Cas has revolutionized genome editing and has been applied in biomedical research, agricultural improvement, and as a diagnostic tool for disease detection and screening.
What are the potential applications of CRISPR Cas in medicine?5 answersCRISPR/Cas systems have potential applications in medicine, including disease prevention, mechanism research, and gene treatment of diseases. They can be used for vaccine design, trait improvement, animal models of human diseases, organ transplantation, gene therapy, and gene detection. Specifically, in the field of cancer, CRISPR/Cas9 has shown promise in treating ovarian cancer by targeting oncogenes involved in its pathogenesis. CRISPR/Cas systems also have potential in gene therapy for various human disorders, including cancer and infectious diseases. In the treatment of viral diseases, CRISPR/Cas systems can be used to block virus invasion, interfere with virus replication, and eliminate viral genome and sequelae of virus infection. However, there are challenges that need to be addressed, such as off-target DNA cleavage and effects on non-target, normal cells. Overall, CRISPR/Cas systems have great potential to advance medical research and practice, but safety and efficacy should be carefully regulated and supervised.
How can CRISPR technique?5 answersThe CRISPR technique is a method for modifying a target nucleotide sequence in a cell. It involves introducing a vector system or an expression cassette system containing specific DNAs and a crRNA into the cell. The vector system or expression cassette system consists of multiple components, including regulatory elements that regulate the transcription of the DNAs. The CRISPR/Cas systems, which are RNA-guided endonucleases, have been used for various applications such as genome editing, protein detection, and transcriptional modulation. The technique allows for the precise modification of targeted genes in plant, animal, and human cells, and can also be used to reduce or increase the expression of selected genes. Additionally, CRISPR-based amplification strategies have been developed for accurate estimation of proteins and high-throughput assays. The CRISPR technique has the potential to revolutionize biological research, but ethical and moral issues surrounding its use need to be addressed.
Can crispr cas be used to treat cancer?3 answersCRISPR-Cas9 has shown potential as a treatment for cancer. It has been used to edit genes involved in the pathogenesis of ovarian cancer, such as BMI1, CXCR2, MTF1, miR-21, and BIRC5. CRISPR-Cas9 has also been successful in treating various types of cancers, including aggressive ones, and has shown proof of principle in oncological research. The use of CRISPR-Cas9 in cancer therapy involves modifying the CRISPR-Cas system and optimizing its delivery. Additionally, CRISPR/Cas has proven its efficacy in epigenome editing, which is relevant to cancer therapy. The use of nano-vehicles in combination with CRISPR/Cas has been explored as a potential approach for cancer therapy. Overall, CRISPR-Cas9 holds promise as a treatment for cancer and further research is needed to fully understand its capabilities and optimize its use in clinical settings.
Does Crispr work on RNA viruses?10 answers
What RNA is used in Crispr?10 answers

See what other people are reading

What factors influence flu vaccination behavior among individuals in India?
4 answers
Factors influencing flu vaccination behavior among individuals in India include low awareness of vaccine benefits, safety concerns, mistrust in government and healthcare quality. Access, cost, inadequate information, and lack of government endorsement were barriers to vaccine uptake. Social media interactions positively influenced vaccination intention through social support, information exchange, and value co-creation. The presence of committed vaccinators acted as role models, stimulating others to vaccinate and avoiding clustering of susceptible individuals. Among healthcare workers, barriers to vaccination included the perception of being healthy enough not to contract the flu and lack of time, despite awareness and knowledge of vaccine benefits. These diverse factors highlight the need for tailored strategies to improve flu vaccination rates in India.How does the financial crisis affect the student's academic performance?
5 answers
The financial crisis significantly impacts students' academic performance. Studies reveal that financial distress due to economic crises leads to increased dropout probabilities among university students, especially when family financial conditions deteriorate. Financial stress forces students to consider reducing course loads or dropping out, affecting their grades and credit hours. In Greece, the financial crisis has a notable effect on students' lives and decisions to pursue higher qualifications, although the impact on university studies and career prospects is moderate. Additionally, economic crises and poverty in Ogun state contribute significantly to students' academic performance predictors, emphasizing the need for alternate funding sources and poverty alleviation measures to support students effectively.How does consumerism influence individual attitudes towards material possessions?
5 answers
Consumerism significantly impacts individual attitudes towards material possessions. Studies show that consumer behaviors are influenced by various factors such as environmental concerns, mood, personality traits, and societal changes. For instance, research indicates that environmental awareness and concerns about plastic waste directly affect consumers' attitudes towards products. Additionally, mood, particularly openness to feelings, plays a role in how individuals subjectively evaluate their possessions. Material possession love is also a crucial aspect, affecting consumer well-being and leading to complementary purchases. Moreover, societal changes, like the COVID-19 pandemic, have shown mixed effects on materialism, with increased media consumption and stress predicting higher materialistic values. Overall, consumerism intertwines with emotions, personality traits, and societal influences to shape individual attitudes towards material possessions.What are the effects of academic pressure on students’ mental health in the National Capital Region, Philippines?
5 answers
Academic pressure in the National Capital Region of the Philippines has been shown to significantly impact students' mental health. Studies have highlighted that academic anxiety among senior high school students can lead to moderate to high levels of stress and anxiety. Furthermore, the presence of psychosocial support systems in universities is crucial to address mental health concerns among college students. Research also indicates that academic pressure is associated with adolescent mental health problems like depression, anxiety, self-harm, and suicidality, emphasizing the need for interventions to prevent such issues. Additionally, the COVID-19 pandemic has exacerbated stress levels among graduate students in Metro Manila, with age being a significant factor in assessing global stress levels during the outbreak. These findings underscore the importance of addressing academic pressure to safeguard students' mental well-being in the National Capital Region.What are the common reasons why students face difficulties in math and STEM subjects? pandemic?
5 answers
Students face difficulties in math and STEM subjects during the pandemic due to various reasons. These include inadequate learning materials, lack of support, difficulty in submitting requirements on time, challenges in understanding math material, completing tasks, and receiving support in online learning, affective issues like attitudes towards learning math and emotions impacting success in university STEM courses, lack of basic math knowledge leading to low confidence, lack of interest, motivation, and support in online learning, and struggles in applying calculus knowledge to physics, such as identifying variables for integration and applying rules in physics problems. These intertwined difficulties stem from the complex, abstract nature of mathematics, lack of preparation, and the sudden shift to online learning during the pandemic.What is the important of knowledge about halalan toyyiban food ?
10 answers
Understanding the concept of Halalan Toyyiban is crucial for ensuring food safety, quality, and compliance with Islamic dietary laws, which has significant implications for consumer health, business performance, and environmental sustainability. The Halalan Toyyiban concept, which encompasses both the halal (permissible) and toyyiban (wholesome and pure) aspects of food, is fundamental in Islamic dietary law, emphasizing not only the permissibility but also the quality and safety of food. This dual focus ensures that food consumption adheres to religious mandates while also promoting physical well-being. Knowledge about Halalan Toyyiban food is important because it directly impacts consumer health by preventing the consumption of harmful substances and ensuring that food is safe, nutritious, and clean. The recent Covid-19 pandemic highlighted the dangers of consuming non-halal and non-tayyib food, demonstrating how such practices can lead to widespread diseases and emphasizing the need for a healthy lifestyle that includes the consumption of Halalan Toyyiban products. For businesses, particularly in the food industry, understanding and implementing Halalan Toyyiban principles can significantly enhance marketability and business performance. This is evident in the positive correlation between Halalan Toyyiban awareness, Halal Assurance System (HAS) practices, export readiness, and overall business performance among SMEs in the halal food industry. Moreover, the adoption of Halalan Toyyiban standards can address consumer concerns regarding the halal status of food products, thereby enhancing consumer trust and satisfaction. Environmental sustainability is also a key aspect of the Halalan Toyyiban concept. The approach to food safety within this framework includes considering the environmental impact of food production, such as chemical contamination, and emphasizes the restoration of natural resources. Additionally, in sectors like aquaculture, adopting Halalan Toyyiban principles ensures the production of clean and pure food, contributing to the sustainability of the halal supply chain. In summary, knowledge about Halalan Toyyiban food is essential for ensuring food safety and quality, supporting business performance in the halal food industry, and promoting environmental sustainability.How can molecular methods enhance viral diagnosis?
4 answers
Molecular methods significantly enhance viral diagnosis by providing rapid, sensitive, and specific detection of viral pathogens. Techniques like polymerase chain reaction (PCR) amplify viral genetic material for easy identification, especially in low-concentration samples. Next-generation sequencing (NGS) allows for comprehensive genome analysis, aiding in understanding virulence factors and mutations that impact treatment strategies. Additionally, molecular methods like in situ hybridization and loop-mediated isothermal amplification (LAMP) offer precise localization of viruses in tissues and quick field diagnosis, respectively. These advanced tools not only expedite viral detection but also contribute to the development of effective antiviral therapies and vaccines, crucial for managing emerging viral infectious diseases.How can molecular methods like real time PCR enhance viral diagnosis?
4 answers
Molecular methods like real-time PCR play a crucial role in enhancing viral diagnosis by providing rapid and specific identification of viral pathogens, aiding in effective clinical management. These methods, including PCR, next-generation sequencing (NGS), and CRISPR-Cas, offer various advantages in detecting viruses in clinical samples. Real-time PCR, in particular, allows for monitoring of amplification and quantification of template genes, overcoming the challenges of nonspecific product synthesis. Additionally, automated molecular biology techniques have revolutionized viral testing, providing sensitive and accurate results with faster turnaround times, ultimately improving patient care. The development of gel-based PCR, real-time PCR, and other advanced diagnostic tools further strengthens the arsenal of nucleic acid-based molecular diagnosis for viruses, aiding in the early detection and management of viral diseases.How can colleges and universities promote active lifestyles for students?
5 answers
Colleges and universities can promote active lifestyles for students by implementing evidence-based interventions that target various factors influencing physical activity (PA) levels. Factors such as exercise self-efficacy, PA enjoyment, and recording PA behaviors have been identified as key predictors of PA in college students. Additionally, creating safe community neighborhoods and enhancing land use mix on campus can encourage participation in PA. Innovative interventions, like physical activity programs co-constructed with students, have shown promise in promoting healthier lifestyles and psychological well-being among students. Furthermore, integrating independent physical education classes with daily routines and emphasizing the importance of physical activity can significantly impact the health and physical condition of students. By focusing on these strategies, colleges and universities can effectively promote active lifestyles and combat physical inactivity among their student populations.How should placements be assessed/how? (Pass/fail, graded, employers participation etc)?
5 answers
Placements should be assessed through a multi-faceted approach that considers various perspectives. Employers' participation in the assessment process can provide valuable insights into candidates' performance and suitability for the role. Assessment methods should promote a shared understanding among educators, practitioners, and students to ensure alignment on academic standards. Utilizing data-driven computational approaches, such as process mining and text analytics, can offer valuable insights into students' engagement with workplace-based assessments, aiding in understanding their participation and areas for improvement. Monitoring the quality of placements and surveying students' opinions are crucial tools for enhancing the assessment process and improving the overall quality of education. Ultimately, a comprehensive assessment strategy that incorporates input from all stakeholders and leverages data analytics can lead to more effective evaluation of students during placements.What factors influence flu vaccination behavior among individuals in Singapore?
5 answers
Factors influencing flu vaccination behavior among individuals in Singapore include knowledge, attitudes, and practices (KAP). For children, factors like living in landed property and a history of hospitalization due to cough were associated with higher influenza vaccine uptake, while higher parental education and non-smoking households were linked to increased pneumococcal vaccine uptake. In older adults, socioeconomic factors, information availability, and cultural beliefs play a role in vaccination decisions, with multicomponent interventions showing promise in improving uptake. Among healthcare workers, the COVID-19 pandemic led to increased influenza vaccine uptake, likely due to heightened awareness and anxiety surrounding respiratory diseases and vaccines. Additionally, misperceptions about influenza disease and vaccines, lack of knowledge, and motivation were identified as barriers to flu vaccination among older adults in Singapore.