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The extraction of cyanobacterial slime from community samples and subsequent analysis via GC-MS is an experiment utilizing GC-MS with non-biological samples . This protocol details the process of extracting cyanobacterial slime from community samples and analyzing it using GC-MS, showcasing the application of gas chromatography-mass spectrometry in a non-biological context. While GC-MS is commonly used in metabolomics research with biological samples, this experiment demonstrates its versatility in analyzing non-biological samples like cyanobacterial slime. By focusing on the extraction and analysis of cyanobacterial slime, this experiment highlights the broad applicability of GC-MS beyond traditional biological sample analysis, showcasing its utility in various scientific investigations involving non-biological substances.

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Open access•Journal Article•DOI Chlorine speciation in complex hydrocarbon matrices using GC-ICP-MS/MS Vincent Souchon, Fabien Chainet, Charles-Philippe Lienemann - Show less +2 more 01 Jan 2023-Journal of Analytical Atomic Spectrometry	The experiment described in the paper utilizes GC-ICP-MS/MS for chlorine speciation in complex hydrocarbon matrices, demonstrating a non-biological sample analysis using GC-MS technology.
Open access•Posted Content•DOI Extraction of Cyanobacterial Slime from Community Samples and Subsequent Analysis via GC-MS v1 Montana Valley CBD Gummies 04 Mar 2023	Not addressed in the paper.
Open access•Journal Article•DOI GC-MS analysis of Adulterants in Captagon Tablet Ali Zaid Alshehri, Mohammed saeed Al Qahtani, Mohammed Aedh Al Qahtani, Abdulhadi M Faeq, Jawad Aljohani, Ammar Alfarga - Show less +5 more 31 Dec 2020	GC-MS analysis of Captagon tablets for adulterants was conducted, revealing amphetamine and caffeine presence, showcasing a non-biological sample experiment using GC-MS for substance profiling.
Open access•Journal Article•DOI Matrix Effects in GC–MS Profiling of Common Metabolites after Trimethylsilyl Derivatization Elena Tarakhovskaya, Andrea Marcillo, Caroline J. Davis, Sanja Milkovska-Stamenova, Antje Hutschenreuther, Claudia Birkemeyer - Show less +5 more 01 Mar 2023-Molecules	Not addressed in the paper.
Open access•Journal Article•DOI Experiences and Perspectives of GC-MS Application for the Search of Low Molecular Weight Discriminants of Schizophrenia Natalia Porozova, Elena Danilova, I.V. Senshinov, Andreas Tsakalof, A.Ye. Nosyrev - Show less +4 more 31 Dec 2022-Molecules	Not addressed in the paper.

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What is gc-ms?5 answersGas Chromatography-Mass Spectrometry (GC-MS) is a powerful analytical technique extensively used in various fields such as metabolomics and bioactive compound analysis. GC-MS allows for the simultaneous measurement of a wide range of compounds, including organic acids, amino acids, sugars, and fatty acids. In the context of herbal plants, GC-MS is utilized to identify bioactive compounds like alkaloids, terpenoids, flavonoids, and glycosides, which are crucial for their therapeutic effects. Moreover, in the study of Sphenocentrum jollyanum, GC-MS was employed to analyze the ethanol extract and identify 45 bioactive compounds, showcasing its utility in determining the composition of plant extracts. Overall, GC-MS plays a vital role in identifying and quantifying compounds in complex mixtures, making it an invaluable tool in research and diagnostics.

Disadvantages of GC-FID-MS analysis method for food analysis?5 answersThe GC-FID-MS analysis method for food analysis has some disadvantages. One key issue is the potential for false positives due to interferences that may not be effectively filtered out by GC-MS. Additionally, in the presence of compounds like nitrite, the conventional assays may lead to underestimation of lipid oxidation, affecting the accuracy of results. Moreover, the limitations of GC-MS include the need for careful interpretation of chromatograms to avoid including hydrocarbons of natural or synthetic origin, which can lead to faulty data. Despite its high resolution and sensitivity, GC-MS may not always provide the required selectivity for certain food analysis applications, potentially impacting the accuracy and reliability of the results.

What are the advantages of GC-MS for compound determination?4 answersGas chromatography-mass spectrometry (GC-MS) has several advantages for compound determination. It is well regarded for its ability to separate mixtures, making it a valuable tool in identifying seized drugs. GC-MS can provide more selective analysis through derivatization techniques, enhancing chromatographic results and allowing for the characterization of compounds that have similar mass spectral data. It offers reduced peak tailing and increased abundance after derivatization, improving the detection and identification of compounds. GC-MS also allows for the analysis of a wide range of compounds, including psychoactive substances, amphetamines, synthetic cathinones, and polycyclic aromatic hydrocarbons (PAHs). Additionally, GC-MS can be a less expensive and more ecologically friendly alternative to liquid chromatography-mass spectrometry (LC-MS) for the detection of certain compounds. Overall, GC-MS is a versatile and effective technique for compound determination in various fields, including forensic science and environmental analysis.

What are the different applications of GC-MS?4 answersGas chromatography-mass spectrometry (GC-MS) has various applications in different fields. In biotechnology research, GC-MS is used to analyze herbal plants and identify bioactive compounds. In metabolomics and exposomics, high-resolution accurate mass (HRAM) GC-MS systems like GC-Orbitrap-MS are used for capturing metabolites and exposures. GC-IMS, a type of GC-MS, is used for breath analysis and has potential in clinical areas such as screening for pulmonary diseases and infections. GC-MS is also used for metabolic screening and profiling of amino acid transporters, providing insights into their substrate specificity and role in intracellular signaling pathways. Additionally, GC-MS is used in state-of-the-art applications such as food aroma analysis, food contamination detection, petrochemical analysis, environmental contamination analysis, and human fluid metabolomics.

Can GC-MS be used to analyze the composition of ghee?5 answersGC-MS can be used to analyze the composition of ghee. Various studies have used GC-MS to characterize the fatty acid composition of ghee samples. The fatty acid profile of ghee residue was analyzed using GC-MS, revealing the presence of saturated fatty acids such as palmitic acid and unsaturated fatty acids such as oleic acid. Additionally, GC-MS was used to identify and quantify the flavor compounds in Tibet Ghee, with 34 components being identified. Furthermore, GC-MS analysis of headspace volatiles was used to understand the flavor differences between different types of ghee, including Desi Ghee and industrial Ghee. In summary, GC-MS is a valuable analytical technique for studying the composition of ghee, including its fatty acid profile and flavor compounds.

What are the advantages and limitations of using GC-MS for fluorine compounds detection?5 answersGC-MS is a useful technique for the detection of fluorine compounds. It offers several advantages such as high sensitivity, no background signal, and a large chemical shift range. However, there are also limitations to using GC-MS for fluorine compound detection. One limitation is the broad linewidths of fluorine, which can complicate spectral analysis and accurate peak deconvolution. Another limitation is the need for careful control of reactions with offsite cysteines to avoid spectral simplification and accurate identification of component peaks. Additionally, the use of detergent micelles can lead to non-covalent probe sequestration, which can affect the accuracy of fluorine compound detection. Overall, while GC-MS is a valuable tool for fluorine compound detection, it is important to consider these limitations and optimize experimental protocols to ensure accurate and reliable results.

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