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How calculate IC50 of ACacia senegal and boswellia sacra resin on cell line cancer cells Caco-2?

Boswellia sacra

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The IC50 values for Acacia senegal and Boswellia sacra resin on cancer cells can be determined through cytotoxicity assays like the MTT assay. For Acacia senegal, in vitro cytotoxic potential was observed against various cancer cell lines, including cervical, neuroblastoma, and lung cells, with significant effects at concentrations of 30 and 100 μg/ml . On the other hand, Boswellia sacra resin extracts exhibited cytotoxicity against metastatic breast cancer cells, with the resin of B. sacra showing the most potent effects with an average IC50 of 8.3 ± 0.6 µg/mL . These values indicate the concentration at which 50% of the cancer cells are inhibited, providing crucial information for assessing the efficacy of these natural products against cancer cells like Caco-2.

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Open access•Journal Article•DOI Essential Oils Extracted from Boswellia sacra Oleo Gum Resin Loaded into PLGA–PCL Nanoparticles: Enhanced Cytotoxic and Apoptotic Effects against Breast Cancer Cells H. Azzazy, Anwar Abdelnaser, Hadeer Al Mulla, Amany M. Sawy, Samir Shamma, Mahmoud Elhusseiny, S. Alwahibi, N. Mahdy, Sherif A. Fahmy - Show less +8 more 19 Dec 2022-ACS Omega 2 Citations	Not addressed in the paper.
Open access•Journal Article•DOI Acacia senegal Possesses in vitro Cytotoxicity Against Human Cancer Cells Vikas Sharma 01 Oct 2016-International Journal of Medical Sciences	Not addressed in the paper.
Open access•Journal Article•DOI Sacraoxides A-G, Bioactive Cembranoids from Gum Resin of Boswellia sacra . Bingyang Zhang, Di Liu, Wenyue Ji, Kouharu Otsuki, Koji Higai, Feng Zhao, Wei Li, Kazuo Koike, Feng Qiu - Show less +8 more 30 Mar 2021-Frontiers in Chemistry 4 Citations	Not addressed in the paper.
Journal Article•DOI Impact of Surfactants on Formulation Parameters and in vitro cytotoxicity of Boswellic Acids Loaded Nanoparticles on Human Colon Cancer Cell Lines Harish Dureja Neeta 19 Nov 2018-Indian Journal of Pharmaceutical Education and Research 3 Citations	Not addressed in the paper.
Open access•Journal Article•DOI Comparative Analysis of Pentacyclic Triterpenic Acid Compositions in Oleogum Resins of Different Boswellia Species and Their In Vitro Cytotoxicity against Treatment-Resistant Human Breast Cancer Cells. Michael Schmiech, Sophia J. Lang, Katharina Werner, Luay J. Rashan, Tatiana Syrovets, Thomas Simmet - Show less +5 more 07 Jun 2019-Molecules 28 Citations	Not addressed in the paper.

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What does IC50 measure?5 answersIC50 measures the potency of a drug or therapeutic agent by determining the concentration at which it inhibits a specific biological process by 50%. It is utilized across various fields, such as cancer research, carbohydrate inhibition studies, and drug-induced proarrhythmicity assessments. In cancer research, IC50 is used to assess the effectiveness of chemotherapeutic drugs on cancer cell viability, with seeding density affecting drug response. Additionally, IC50 is employed in carbohydrate studies to evaluate the efficiency of inhibiting lectin binding. Moreover, in drug-induced proarrhythmicity evaluations, IC50 quantifies the potency of drugs in blocking hERG channels, with protocol variations impacting measurements. Overall, IC50 serves as a crucial metric for determining the efficacy and potency of various compounds in different biological contexts.

HOW TO CALCULATE IC50 of acacia senegal on cell line?5 answersTo calculate the IC50 of Acacia senegal on a cell line, such as keratinocytes or cancer cells, a dose-response curve can be constructed by testing various concentrations of the plant extract. For instance, in the context of keratinocytes, the IC50 was found to be 1302 ± 60 μg/mL. Similarly, in the context of cancer cells, the effective concentration (EC50) was determined to be 40 μg/mL for Acacia nilotica leaf ethanolic extract. By evaluating the cytotoxic activity at different concentrations and plotting the results, the concentration at which 50% inhibition of cell growth occurs can be extrapolated as the IC50 value, indicating the potency of the plant extract against the specific cell line.

What are the effects of Calocybe indica on cancer cells?5 answersCalocybe indica has been found to have various effects on cancer cells. The polysaccharides extracted from Calocybe indica showed antioxidant and antitumor activities, as well as anticoagulant effects. Another study found that crude polysaccharides from Calocybe indica exhibited antioxidant activity and antiproliferative effects on cancer cell lines. Furthermore, the ethanolic extract of Calocybe indica was shown to have anti-inflammatory and anticancer properties, inhibiting the growth of breast cancer cells. In vitro studies on pancreatic cancer cell lines also demonstrated that the ethanolic extract of Calocybe indica inhibited cell growth, induced apoptosis, and inhibited cell migration. Additionally, the fruiting body extract of Calocybe indica showed potent anticancer effects on cervical cancer cells, inducing apoptosis, arresting the cell cycle, and inhibiting colony formation and cell migration. These findings suggest that Calocybe indica has potential as a natural anticancer agent.

What is the mechanism of action of Boswellia serrata?5 answersBoswellia serrata, commonly known as frankincense, has a diverse mechanism of action. It has been found to block various inflammatory pathways, making it beneficial for treating conditions such as gingivitis, asthma, diabetes, and inflammatory bowel diseases. Boswellic acids, the major constituents of Boswellia serrata, have been shown to have anti-cancer activity and can potentially be used as safe and effective anti-cancer drugs. Boswellia serrata extract has also been found to have potential therapeutic effects against COVID-19, including antioxidant, anti-inflammatory, immunomodulatory, and antiviral activities. Additionally, Boswellia serrata extract has demonstrated antifungal activity against Candida species, making it a potential treatment for infections caused by these pathogens. Furthermore, Boswellia serrata has been shown to modulate the gut microbiome composition, with effects on bacterial richness and the abundance of specific bacteria such as Akkermansia muciniphila and Bifidobacterium.

What is the germination rate of boswellia sacra?5 answersThe germination rate of Boswellia sacra varies depending on the source location and external ecological factors. The low rate of genetic variation among wild stands and the common practice of vegetative propagation through tree cuttings in cultivated stands contribute to the low germination rate. Seed germination rates were observed to differ among Boswellia species, localities, and years, indicating the influence of external ecological factors on seed development. The resin and essential oil of Boswellia sacra have been found to inhibit aflatoxin production by Aspergillus species, suggesting their potential as bioreservatives. Genetic diversity analysis using DNA sequences and ISSR markers showed low variation among geographically different populations of Boswellia sacra, with differentiation observed between eastern and western coastal populations.

How does IC50 mechanisms inhibits cancer cell line proliferation?5 answersIC50 is a measure of the concentration of a drug that is required to inhibit the proliferation of cancer cells by 50%. The mechanisms by which IC50 inhibits cancer cell line proliferation vary depending on the specific drug being used. In the case of carbendazim, it inhibits proliferation and induces mitotic arrest in breast cancer cells by suppressing microtubule dynamic instability. Chalcone-3, on the other hand, inhibits proliferation of breast cancer cells by reducing the expression of pEGFR and cyclin-D1, leading to cell cycle arrest. Ursolic acid inhibits proliferation and induces apoptosis in gastric cancer cells by arresting the cell cycle, inhibiting COX-2 expression, and down-regulating Bcl-2 expression. Sarang Semut, a plant extract, demonstrates anti-tumor activity in colon cancer cells by inducing cell death and inhibiting cell growth and colony formation. Overall, IC50 mechanisms inhibit cancer cell line proliferation by targeting specific cellular processes and pathways involved in cell growth and division.

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