How can you grow Mycobacterium tuberculosis in laboratory?
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34 Citations | These findings indicate that positive M. tuberculosis cultures resulting from laboratory contamination can occur. |
| In order to carry out the right treatment and epidemiologic evaluation, it is important to identify non-tuberculosis mycobacterium by culture methods. | |
| As the incidence of tuberculosis declines, more resources will be used inappropriately for patients whose culture specimens grow mycobacteria other than M. tuberculosis. | |
| Mycobacterium tuberculosis might have originated from an environmental ancestor. |
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What is the growth rate of Mycobacterium tuberculosis?5 answersThe growth rate of Mycobacterium tuberculosis (M. tuberculosis) varies depending on the conditions. Studies have shown that M. tuberculosis can have a mean generation time ranging from 23.1 hours for fast-growing bacilli to 69.3 hours for slow-growing ones. Additionally, exposure to tobacco smoke condensate has been found to stimulate the growth and biological activities of M. tuberculosis, resulting in accelerated growth and increased bacterial mass. Furthermore, isothermal microcalorimetry experiments revealed that in serum-supplemented urine, M. tuberculosis exhibited a growth rate of 0.008 [h^-1], with enhanced biofilm formation. Understanding the growth rates of M. tuberculosis is crucial for developing effective treatment strategies and combating this pathogen.
What is the genus Mycobacterium?3 answersThe genus Mycobacterium is a group of non-motile, non-spore-forming, and aerobic bacilli that includes both pathogenic and non-pathogenic species. Mycobacteria are slightly curved rods that can exhibit filamentous and branching forms. They are named after the word "mould" due to their ability to form mould-like pellicles in liquid medium. Mycobacterium tuberculosis, the causative agent of tuberculosis, is one of the pathogenic species within this genus. There has been a debate about splitting the genus Mycobacterium into five new genera, but recent studies support the original genus label for all species within this group. Mycobacterium avium subspecies paratuberculosis is a pathogenic species that affects cattle and wildlife, causing paratuberculosis or Johne's disease. Non-tuberculous mycobacteria, which have a wide host range, are also of medical relevance, particularly in individuals with compromised immune systems.
How to cultivate bacterial strain?5 answersBacterial strain cultivation can be done using various methods and devices. One method involves preparing a culture medium by boiling sterile water and adding brown sugar, peptone, potassium dihydrogen phosphate, and vitamins. The mixture is then cooled and a strain is added, followed by standing for 1-2 days before transferring to a complete set device for shake culture. Another method involves cultivating strains that can survive in extreme environments and then inoculating them onto the roots of selected plants. This method increases plant biomass, drought resistance, and survival rate. However, the cultivation of microorganisms by traditional methods is limited, and the majority of microbial diversity remains uncultured. Different cultivation strategies are required based on the scientific goals, such as using high-throughput non-standard methods for isolating strains representing higher ranking taxa. Additionally, there are specific devices available for bacterial strain cultivation, such as a bacterial strain culture device that allows for adjusting the height according to the growth of the strain, and a bacterial strain electric acclimatizing equipment that provides a controlled environment for culturing strains.
Why is fatty acid biosynthesis important for mycobacterium tuberculosis?3 answersFatty acid biosynthesis is important for Mycobacterium tuberculosis (Mtb) because it plays a crucial role in the pathophysiology and survival of the bacteria. The mycolic and fatty acids are the primary lipid components of the mycobacterial cell envelope, which protect the bacteria from antibiotic susceptibility and help them evade the host immune system. Proteins involved in the lipid biosynthesis pathway, such as Rv2881c and Rv2764c, are essential for lipid synthesis and are potential drug targets for Mtb. Lipids are also stored as intrabacterial lipid inclusions (ILI), which serve as a critical source of energy for Mtb under nutrient-limiting conditions, promoting bacterial survival and persistence. The dynamics of ILI metabolism and lipid homeostasis are important for Mtb adaptation, survival, and virulence during infection. Understanding fatty acid biosynthesis and lipid metabolism in Mtb can provide insights for the development of effective treatment strategies against tuberculosis.
How to culture mycobacterium tuberculosis?5 answersMycobacterium tuberculosis can be cultured using various methods. One method involves using an anti-contamination culture tube with a horizontal design to uniformly distribute the nutrient solution and prevent excessive accumulation or sparsity of the solution. Another method is a quick separation and culture method that includes anti-pollution liquefaction treatment, adding a foreign bacteria inhibitor to a liquid culture medium, inoculating the specimen solution onto the culture medium, and performing suspension cultivation at a constant temperature. Additionally, a culture medium for mycobacterium tuberculosis can be prepared using components such as banana pulp, pig lung homogenate, cow spleen homogenate, watermelon juice, grape juice, and milk, which provide complex nutrition and energy for bacterial growth. Furthermore, a culture medium can be formed by mixing solid and liquid culture media, such as L-G medium and 7H9 culture medium, and enriching the Mycobacterium tuberculosis specimen in the culture vessel.
What are the laboratory practices for mycobacterium tubercolosis?2 answersLaboratory practices for the diagnosis of Mycobacterium tuberculosis include various techniques such as acid-fast staining, rapid-cycle PCR, Xpert MTB/RIF assay, culture, DNA probes, broad-range PCR with DNA sequencing, high-performance liquid chromatography, matrix-assisted laser desorption/ionization-time of flight mass spectrometry, susceptibility testing, and strain typing. These practices are used to detect, identify, and characterize M. tuberculosis in clinical specimens. The World Health Organization encourages the use of sensitive and accurate techniques like Gene Xpert, interferon-gamma release assay (IGRAs), and urinary antigen analysis for routine laboratory diagnosis, especially in high TB burden countries. State laboratories play a significant role in mycobacterial testing services, including species identification, culture, and drug susceptibility testing, while hospital and commercial laboratories often refer specimens to state laboratories for testing. Molecular methods are considered the gold standard for rapid detection and identification of M. tuberculosis, and they are also used for drug resistance testing. The use of nucleic acid amplification tests has been included in the diagnostic criteria for tuberculosis in some countries.