How does calcium phosphat transfection work?
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Calcium phosphate-mediated transfection involves the use of calcium phosphate coprecipitated with DNA to introduce genetic material into cells. This method is versatile, with variations for different cell types. It has been utilized for over two decades and has shown efficacy in both in vitro and in vivo settings, with factors like the amount of powder and contact time influencing transfection efficiency. The mechanism of transfection is not fully understood but may involve the growth of carbonated apatite on ceramic surfaces. This technique has been explored for transfecting high-molecular-weight genomic DNAs. Overall, calcium phosphate-mediated transfection offers a promising approach for introducing genetic material into cells, with potential applications in various fields like tissue engineering and DNA vaccines.
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3 Citations | Calcium phosphate transfection optimizes gene transfer in Mesenchymal Stem Cells by providing a safe and effective method for diverse biological processes in MSC therapies. |
11 Citations | Calcium phosphate-mediated transfection involves using plasmid DNAs and adherent cells for gene delivery. The protocol also includes an alternative method for generating stable transfectants with high efficiency. |
| The mechanism of calcium phosphate transfection is possibly linked to carbonated apatite growth on ceramic surfaces, enhancing cell transfection efficiency, particularly in bone tissue engineering and DNA vaccines. | |
| Calcium phosphate transfection involves forming a coprecipitate of DNA with calcium phosphate, which is then added to cells for gene delivery, enhancing transfection efficiency in adherent or suspension cell cultures. | |
5 Citations | Calcium phosphate-mediated transfection facilitates the transfer of high-molecular-weight genomic DNA into cells, as outlined in the protocol provided in the paper. |
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