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How to estimate lateral flow? 


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Lateral flow assays can be estimated using various methods and techniques. One approach is to use a device that includes a receptacle with a compartment for holding a lateral flow assay test strip, a liquid passageway, and a lid with a sample receiving portion . Another method involves using lateral flow test strips that can decomplex the analyte from the analyte-antibody complex during the assay, resulting in high-quality assays without the need for a decomplexation pre-treatment step . Additionally, a lateral flow assay device can be configured with a removable gap between the conjugate pad and the membrane, allowing the fluid to flow into the membrane and the test line by capillary action . Continuous monitoring of the assay until the detection of an analyte can be achieved using a surface with a reflectance profile or an optical detector for continuous image detection . Finally, a semi-quantitative lateral flow assay device with a porous substrate and multiple reaction zones can be used to generate semi-quantitative data based on the presence of different amounts of analyte .

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Papers (3)Insight
Patent
09 Oct 2014
24 Citations
The paper describes improved methods for estimating lateral flow using test strips that can decomplex analytes from analyte-antibody complexes during the assay.
The device described in the text provides a method for estimating lateral flow using a receptacle, compartment, liquid passageway, and lid.
The paper describes a lateral flow assay device with a removable gap that allows controlled flow of fluid from the conjugate pad to the membrane.

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