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How to make Bacterial inoculation preparation in 2023? 


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In 2023, bacterial inoculation preparations can be made using various methods outlined in recent research papers. One approach involves culturing microorganisms in a growth medium, mixing the culture with a carrier, and air-drying the mixture slowly to reduce moisture levels and form a dried composition . Another method includes culturing nitrobacteria spores with glucose solution, Tween-20, and Tween-80 to create a nitrobacteria inoculation liquid . Additionally, a technique for producing agriculturally useful inoculants of dried, dormant bacteria suggests maintaining a bacterial suspension at a specific temperature, mixing it with a carrier, and air-drying the mixture under aseptic conditions . These methods offer simplicity, convenience, and effectiveness in preparing bacterial inoculation products for various agricultural applications.

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The preparation method in 2023 involves activating specific bacterial strains, culturing them to obtain seed bacterium suspensions, mixing, and fermenting until spore concentration reaches (2-5)*10 CFU/ml.
The method involves isolating microorganisms, processing with infrasonic waves, seeding in nutrient medium, and cultivating without pure cultures. This innovative approach simplifies bacterial inoculation preparation in 2023.
The process involves culturing microorganisms in a growth medium, mixing with a carrier, incubating, and air drying to produce a bacterial agricultural product with high viability for inoculation.
The preparation method for nitrobacteria inoculation liquid in 2023 involves culturing nitrobacteria spores on a PDA panel and mixing with glucose solution, Tween-20, and Tween-80.
Patent
26 Jun 1987
41 Citations
To make a bacterial agricultural inoculant in 2023, maintain a bacterial suspension at 0°-30°C, mix with a carrier at a ratio of 0.5-1.0, and air dry for 2-10 days.

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