How to reduce contamination in the lab during protein purification?
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To reduce contamination during protein purification in the lab, several methods can be employed. One approach is to add surfactants or non-surfactant, non-ionic agents to protein solutions to dissociate or prevent the formation of protein aggregates, which can help in reducing fouling of ultrafiltration membranes . Additionally, incorporating certain types of carbonaceous materials in protein purification processes can effectively remove undesirable impurities without affecting the yield of the desired protein product . Furthermore, eliminating host cell proteins (HCPs) by disrupting multiple genes in Chinese hamster ovary (CHO) cells can significantly reduce total HCP content, leading to improved protein titer and purity during purification processes . These strategies collectively contribute to minimizing contamination and enhancing the efficiency of protein purification in laboratory settings.
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05 Dec 2014 | To reduce contamination during protein purification, utilize a three-step strategy: capture, intermediate separation, and final polishing, removing impurities progressively to achieve high purity efficiently. |
| Multiplex secretome engineering in CHO cells by disrupting multiple genes reduces host cell protein contamination, enhancing protein purity during purification processes in the lab. | |
| To reduce contamination during protein purification, add a surfactant or non-surfactant, non-ionic agent to the protein solution to prevent protein aggregation and fouling of ultrafiltration membranes. | |
Patent 09 Aug 2012 4 Citations | Novel protein purification methods using specific carbonaceous materials effectively remove impurities without compromising protein yield, reducing contamination risks in the lab during purification processes. |
Patent 02 Aug 2012 23 Citations | Novel protein purification methods using specific carbonaceous materials effectively remove impurities without compromising protein yield, reducing contamination in the lab during protein purification. |
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