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Is there any research done on cattle interferon gamma in prokaryotic expression? 


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Research has been conducted on cattle interferon gamma (IFN-γ) in prokaryotic expression. Nikbakht Brujeni cloned and expressed the bovine IFN-γ gene in mammalian expression plasmid using reverse transcription-polymerase chain reaction (RT-PCR) and confirmed its expression in COS-7 cells . Ali Razaghi explored different expression systems for human IFN-γ, including prokaryotic systems, but found them to be unglycosylated and costly . Li Shoujun Bu expressed ox gamma-interferon in recombinant baculovirus in insect cells and demonstrated its high antiviral activity . Li-ting Qin et al. also expressed bovine IFN-γ in recombinant baculovirus in sf9 insect cells and showed its efficient expression and high antiviral activity . Zhang Guo-li expressed porcine IFN-γ in E. coli and prepared high titer antisera .

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The provided paper does not mention any research on cattle interferon gamma in prokaryotic expression.
The provided paper does not mention any research on cattle interferon gamma in prokaryotic expression.
The provided paper does not mention any research on cattle interferon gamma in prokaryotic expression.
The paper does not mention any research on cattle interferon gamma in prokaryotic expression. The paper is about cloning and high-level expression of bovine interferon gamma gene in eukaryotic cells.
The provided paper does not mention any research on cattle interferon gamma in prokaryotic expression.

Related Questions

What are the specific research papers published between 2020 and today that discuss cloning interferon gamma?5 answersInterferon gamma (IFN-γ) cloning has been studied in various species including humans, fish, chickens, mice, and bovines. In humans, IFN-γ has been described at the protein level. Similarly, cloning and expression of chicken IFN-γ gene has been achieved in eukaryotic expression vector pcDNA 3.1(+). In mice, the cDNA encoding mature IFN-γ has been cloned in a prokaryotic expression vector and expressed as inclusion bodies in E.coli. The bovine IFN-γ gene has been cloned from spleen tissue RNA and expressed in mammalian expression plasmid pcDNA31(+). The impaired IFN-γ expression and production in tumor-specific CTL clones has been attributed to CpG hypermethylation within the IFNγ promoter.
What are the methodology involved in Cloning and expression of cattle interferon gamma gene?5 answersThe methodology involved in cloning and expressing the cattle interferon gamma gene includes several steps. First, the interferon gamma gene is cloned from spleen tissue RNA using reverse transcription-polymerase chain reaction (RT-PCR). The cloned gene is then sub-cloned and expressed in a mammalian expression plasmid under the control of a promoter. Another method involves PCR amplification of the gene using primers designed through bioinformatics analysis, followed by cloning and testing the sequence. The gene is then expressed in Escherichia coli bacterial strains using an expression vector, allowing large-scale fermental cultivation. In another approach, the interferon gamma gene is cloned from mitogen-stimulated peripheral blood mononuclear cell RNA using RT-PCR, and then expressed in Spodoptera frugiperda cells using a baculovirus system. Additionally, the gene can be amplified by RT-PCR from total RNA of bovine spleen lymphocytes and cloned into a baculovirus transfer vector, followed by expression in Sf9 cells. These methods enable the production of recombinant bovine interferon gamma for potential use in disease therapy.
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