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Molar ratio can affect the value of KM constant in a cascade reaction?

Cascade reaction

Carboxymethyl cellulose

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The molar ratio can affect the value of the KM constant in a cascade reaction. The Michaelis constant KM is a measure of the affinity of an enzyme for a specific substrate. In the context of enzyme kinetics, it is shown that KM may vary with ligand concentration depending on the mechanism involved . Additionally, the application of robotic systems to study complex reaction kinetics in cascade reactions has practical problems in calculating the rate constants k1 and k2 from concentration measurements . Theoretical limitations of the quasi-steady-state approximation are discussed in relation to defining the KM value . Furthermore, a machine and deep learning model successfully predicts KM values for natural enzyme-substrate combinations, providing genome-scale predictions for model organisms . Overall, the molar ratio can influence the KM constant in a cascade reaction, and understanding the specific mechanism and using predictive models can aid in estimating KM values.

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Open access•Journal Article•DOI Deep learning allows genome-scale prediction of Michaelis constants from structural features. Alexander Kroll, Martin K. M. Engqvist, David Heckmann, Martin J. Lercher - Show less +3 more 01 Oct 2021-PLOS Biology 22 Citations	The provided paper does not mention anything about the molar ratio affecting the value of the KM constant in a cascade reaction.
Open access•Journal Article•DOI Effect of KM Molar Mass on CMC-KM-Acid Gel Takamitsu Kasahara, Machiko Takigami, Naotsugu Nagasawa, Shoji Takigami - Show less +3 more 01 Sep 2009-Transactions-Materials Research Society of Japan	The provided paper does not mention anything about a cascade reaction or the effect of molar ratio on the KM constant.
Open access•Journal Article•DOI The apparent Km is a misleading kinetic indicator I W Plesner 01 Oct 1986-Biochemical Journal 16 Citations	The provided paper does not directly address the effect of molar ratio on the value of the KM constant in a cascade reaction.
Open access•Posted Content•DOI The relative values of the turnover number and the dissociation rate constant determine the definition of the Michaelis-constant Nazzal G 11 May 2016-bioRxiv 2 Citations	The provided paper does not discuss the effect of molar ratio on the value of the KM constant in a cascade reaction.
Journal Article•DOI Kinetic studies of cascade reactions in high-throughput systems. David Iron, Hans F. M. Boelens, Johan A. Westerhuis, Gadi Rothenberg - Show less +3 more 01 Dec 2003-Analytical Chemistry 6 Citations	The provided paper does not discuss the effect of molar ratio on the value of the KM constant in a cascade reaction.

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