What are Decellularized scaffolds?
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Decellularized scaffolds are biomaterials that have been treated to remove cellular components while preserving the extracellular matrix (ECM) structure. These scaffolds provide a framework for cell attachment, proliferation, and tissue regeneration. Lena Mungenast et al. used decellularized spinal cord ECM (dECM) to create electrospun fiber scaffolds for spinal cord regeneration. René García-Contreras et al. decellularized nopal (Opuntia Ficus-indica) to create scaffolds for tissue engineering and regenerative medicine. Jagadeesh Kumar Reddy Mutra et al. discussed the methodology for making scaffolds directly from natural materials without extracting substances from them. Francesco Giovanniello et al. investigated the mechanical properties of decellularized porcine aortas for aortic grafts. Ho-Seung Kang et al. used decellularized squid mantle scaffold (DSMS) as a potential tissue-engineered corneal stroma material. Overall, decellularized scaffolds offer a promising approach for tissue engineering and regenerative medicine by providing a biocompatible and biomimetic environment for cell growth and tissue regeneration.
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2 Citations | Decellularized scaffolds are obtained by removing cellular components from tissues, such as porcine and human aortas, while preserving the extracellular matrix structure. |
| Decellularized scaffolds are scaffolds made from tissues that have had their cellular components removed, leaving behind the extracellular matrix (ECM) components. | |
| Decellularized scaffolds are structures that have had their cellular components removed, leaving behind a framework that can be used for tissue engineering and regenerative medicine. | |
| Decellularized scaffolds are discussed in the paper as one of the prominent methods employed for scaffold preparation in tissue engineering. |
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How to characterize decellularized amniotic membrane or decllularized and micronized amniotic membrane?5 answersDecellularized amniotic membrane can be characterized using various methods. Morphological studies using light microscopy and scanning electron microscopy can be performed to assess the preservation of the biomaterial structure and the degree of decellularization. Raman spectroscopy can be used to analyze the spectral lines of the biopolymer and identify the presence of specific biological substances. Histology can be conducted to evaluate the structure of the decellularized membrane and compare it with the native membrane. Hematoxylin and eosin staining, Masson's trichrome staining, and scanning electron microscopy can provide insights into the extracellular matrix composition and the removal of native cells. Physico-chemical evaluations such as differential scanning calorimetry, tensile testing, and Fourier transform infrared spectroscopy can be used to assess the stability and intact structure of the decellularized membrane. In vitro studies can be conducted to evaluate the biocompatibility and cell proliferation on the decellularized membrane.
How does decellularized amniotic powder affect the proliferation of adipose stem cells in vitro?5 answersDecellularized amniotic powder has been shown to stimulate the proliferation of adipose stem cells in vitro. Treatment with soluble extracts of decellularized human amnion/chorion membrane (dHACM) tissue, which is derived from amniotic membranes, has been found to promote the proliferation of adipose-derived stem cells (ADSCs). In addition, dHACM treatment has been shown to enhance the migration of ADSCs. The decellularization process of amniotic membranes does not affect the presence of extracellular matrix (ECM) components in the stromal layer, which may play a role in promoting stem cell activity. These findings suggest that decellularized amniotic powder can positively impact the proliferation of adipose stem cells in vitro, potentially making it a valuable tool for stem cell-based therapies and wound healing applications.
How does decellularized amniotic powder affect the differentiation of adipose stem cells in vitro?5 answersDecellularized amniotic powder has been shown to affect the differentiation of adipose stem cells (ASCs) in vitro. The decellularized placental sponge (DPS) derived from amniotic tissue has been found to guide the osteogenic differentiation of ASCs when co-cultured with macrophages. Additionally, the cell-secreted extracellular matrix (ECM) derived from ASCs during adipogenic differentiation has been shown to play a role in regulating ASC behavior. The ECM secreted by ASCs during adipogenesis undergoes remodeling, with a conversion from fibronectin-rich ECM to laminin-rich ECM. Reseeding ASCs on decellularized ECM (d-ECM) has been found to promote ASC migration when reseeded on growth d-ECM and adipogenic differentiation when reseeded on adipogenic d-ECM. These findings suggest that decellularized amniotic powder can modulate the differentiation of ASCs in vitro, providing a potential strategy for precise regulation of ASC function in adipose tissue engineering.
Do the components of decellularized amniotic membrane have a positive or negative effect on different cell types?4 answersDecellularized amniotic membrane (dAM) components have a positive effect on different cell types. The dAM extract (dAME) showed biocompatibility with corneal epithelial cells, corneal stromal cells, and fibroblasts, promoting normal cell morphology and growth. In the case of human-induced pluripotent stem cells (hiPSCs), dAM matrices improved hepatic differentiation, leading to higher levels of hepatic markers and more stable metabolic functions. The combination of dAM with human adipose-derived mesenchymal stromal cells (AD-MSCs) maintained the stemness and differentiation potential of the cells, allowing them to grow and expand without alterations in cell biology parameters. Decellularized hAM (d-hAM) provided a suitable three-dimensional bioscaffold for culturing human mesenchymal stem cells (hMSCs), with no observable difference in ultrastructural characteristics compared to intact hAM. Decellularized bovine amniotic membranes (BAM) showed high effectiveness in eliminating native cells and preserving the native extracellular matrix (ECM) architecture, making them a potential scaffold for epithelial tissue regeneration.
What is scaffold?5 answersA scaffold is a structure used in construction to provide a safe working area and support for workers. It can be used for various purposes such as bridging steel beams, constructing large-sized wall parts for building external walls, and providing a platform for workers to stand on. Scaffolds can be made of different materials, including steel and polymeric components. They are designed to ensure construction safety, provide a good construction space, and allow for rapid and simple detachment during aloft work. Scaffolds can also be used in tissue engineering for wound healing and tissue regeneration. They are used as carriers for cell and drug delivery, promoting the differentiation of cells and production of growth factors to enhance wound healing. Overall, scaffolds play a crucial role in construction and tissue engineering, providing support, safety, and facilitating various construction and healing processes.