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What are the advantages and disadvantages of different ngs platforms?

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Illumina dye sequencing

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Massive parallel sequencing

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Next-generation sequencing (NGS) platforms offer various advantages and disadvantages. Advantages include high sensitivity, specificity, and cost-effectiveness, making them valuable for applications like precision medicine and genetic diagnosis . NGS platforms, such as Roche 454 GS FLX+ and Illumina MiSeq, have shown the ability to accurately identify pathogens and provide detailed insights into microbial communities in clinical samples . On the other hand, challenges like low-quality base calls at the end of reads can hinder analyses, necessitating pre-processing steps like quality filtering, which can be complex and require deep computer knowledge . Additionally, concerns about security, technology restrictions, and unexpected downtime are disadvantages associated with cloud computing models used in NGS data analysis .

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Papers (5)	Insight
Book Chapter•DOI Next-Generation Sequencing (NGS) Min Wang 01 Jan 2021 1 Citations	Advantages of NGS platforms like Ion Torrent, Illumina, and Complete Genomics include high sensitivity, specificity, and cost-effectiveness. Disadvantages may involve complex procedures impacting accuracy in clinical decision-making.
Open access•DOI Advantages and disadvantages of cloud computing Aldis Plotņikovs, Sergejs Kodors - Show less +1 more 19 Apr 2017 1 Citations	Not addressed in the paper.
Open access•Journal Article Comparison of two NGS platforms for metagenomic analysis of clinical samples Tommaso Biagini, Barbara Bartolini, Emanuela Giombini, Fabrizio Ferrè, Marina Selleri, Gabriella Rozera, Maria Rosaria Capobianchi, Giovanni Chillemi, Alessandro Desideri - Show less +8 more 19 Feb 2017-Journal of Biochemical Technology 3 Citations	Advantages of Roche 454 GS FLX+ and Illumina MiSeq platforms include high-quality assembly of H1N1 virus, while differences exist in depth and genome coverage between platforms.
Proceedings Article•DOI An intelligent genetic platform diagnosing DNA sequencing pathogens by using NGS Christos Bellos, Aleksandros Fyraridis, Georgios S. Stergios, Konstantinos A. Stefanou - Show less +3 more 24 Sep 2021	NGS platforms offer advantages in diagnosing chromosomal abnormalities with higher resolution than aCGH. However, limitations include cost per unit of information and depth of data compared to NGS.
Open access•Journal Article•DOI e-QA NGS: a user-friendly tool to preprocessing data from next generation sequencing Antonio Sérgio Cruz Gaia, Monica S. de Oliveira, Gislenne da Silva Moia, Victória Cardoso Dos Santos, Jorianne Thyeska Castro Alves, Pablo H. C. G. de Sá, Adonney A. O. Veras - Show less +6 more 13 Mar 2023-Peer Review	Not addressed in the paper.

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