What are the common methods for quantitative determination of hormones in urine?
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Common methods for quantitative determination of hormones in urine include solid-phase analytical derivatization (SPAD), ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPHLC/ESI(+)-MS/MS), high performance liquid chromatography–mass spectrometry (HPLC–MS), and a combination of immunoassay and MS/MS. These methods offer sensitive and rapid quantification of various steroid hormones such as testosterone, estrone, progesterone, corticosteroids, and more. Techniques like liquid-liquid extraction, derivatization, and the use of stable isotopes as internal standards enhance the accuracy and efficiency of hormone quantification. The methods provide low limits of detection, high extraction efficiency, and wide linear ranges for multiple hormones, making them valuable tools for understanding hormonal profiles in biological systems and diagnosing endocrine-related disorders.
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| Papers (5) | Insight |
|---|---|
| Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with liquid-liquid extraction and dansylation of estrogens is a common method for quantifying 19 steroid hormones in urine and serum/plasma. | |
| The paper discusses a method utilizing a single antibody for simultaneous capture and MS analysis of multiple hormones, offering a potential approach for quantitative hormone determination in urine. | |
| Not addressed in the paper. | |
| Common methods for quantitative determination of hormones in urine include solid-phase derivatization (SPAD) with trimethylsilyl (TMS) derivatives and conventional liquid-liquid extraction (LLE), with SPAD offering higher recovery rates and shorter preparation time. | |
| The common method for quantitative determination of hormones in urine is ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPHLC/ESI(+)-MS/MS) with multiple reaction monitoring in the positive ionization mode. |
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