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What are the highest HHx contents reached with R. eutropha from P(HB-co-HHx) production?

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Cupriavidus necator

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The highest HHx contents achieved with Ralstonia eutropha in the production of poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)] were within a range of 2–17 mol% without compromising polymer yields. By utilizing a substrate-flexible two-stage fed-batch approach with fructose for biomass accumulation and rapeseed oil for polymer production, a final HHx content of 17 mol% was obtained, along with a polymer content of 86 wt%. The ability to precisely control the HHx content of P(HB-co-HHx) through adjusting substrate ratios highlights the potential for manufacturing tailor-made biopolymers with defined properties. This approach not only allows for customization of the polymer's properties but also contributes to the commercialization of low-cost polyhydroxyalkanoates (PHAs).

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Open access•Journal Article•DOI Recovery of the PHA Copolymer P(HB-co-HHx) With Non-halogenated Solvents: Influences on Molecular Weight and HHx-Content. Moritz Bartels, Björn Gutschmann, Torsten Widmer, Thomas Grimm, Peter Neubauer, Sebastian L. Riedel - Show less +5 more 07 Aug 2020-Frontiers in Bioengineering and Biotechnology 20 Citations	Highest HHx contents reached with R. eutropha for P(HB-co-HHx) production were over 15 mol%, extracted efficiently using acetone and 2-propanol as solvent-precipitant pair.
Open access•Journal Article•DOI A review on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] and genetic modifications that affect its production Huihua Tang, Soon Zher Neoh, Kumar Sudesh - Show less +2 more 05 Dec 2022-Frontiers in Bioengineering and Biotechnology 2 Citations	The highest HHx contents reached with R. eutropha for P(3HB-co-3HHx) production are influenced by factors like PhaCs, PhaCs engineering, and metabolic engineering of strains.
Open access•Journal Article•DOI Substrate-Flexible Two-Stage Fed-Batch Cultivations for the Production of the PHA Copolymer P(HB-co-HHx) With Cupriavidus necator Re2058/pCB113. Lara Santolin, Saskia Waldburger, Peter Neubauer, Sebastian L. Riedel - Show less +3 more 22 Mar 2021-Frontiers in Bioengineering and Biotechnology 9 Citations	The highest HHx content reached with Cupriavidus necator was 18.1 mol% during P(HB-co-HHx) production, demonstrating the potential for high HHx incorporation in the copolymer.
Open access•Journal Article•DOI Tailoring the HHx monomer content of P(HB-co-HHx) by flexible substrate compositions: scale-up from deep-well-plates to laboratory bioreactor cultivations Lara Santolin, Isabel Thiele, Peter Neubauer, Sebastian L. Riedel - Show less +3 more 02 May 2023-Frontiers in Bioengineering and Biotechnology	The highest HHx contents reached with R. eutropha for P(HB-co-HHx) production were up to 36 mol% by genetic modifications, and up to 17 mol% using fructose and canola oil mixtures.

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What feeding strategies exist for P(HB-co-HHx) production?5 answersDifferent feeding strategies have been explored for the production of P(HB-co-HHx) biopolymer. One approach involves utilizing fructose for biomass accumulation and rapeseed oil for polymer production, resulting in high polymer content and productivity yields. Another strategy includes implementing exponential feeding or DO-stat mode with used cooking oil as the carbon source, leading to high P(3HB) volumetric productivity and molecular properties optimization. Additionally, a method has been developed to produce P(HB-co-HHx) using fat extracted from egg waste as a carbon source, demonstrating the potential for high-value bioplastic production and waste reduction. Furthermore, the use of butyrate as a carbon source has enabled the production of P(HB-co-HHx) copolymer with favorable properties in engineered Ralstonia eutropha strains.

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