What are the methods for LNP synthesis?5 answersVarious methods are employed for the synthesis of nanoparticles (NPs). Green chemistry approaches have been proposed to address the toxicity concerns associated with chemically synthesized NPs, offering economical and eco-friendly alternatives. Solvothermal and hydrothermal synthesis techniques enable the controlled formation of low-dimensional materials, allowing for the manipulation of NP morphology and properties. Additionally, DNA-functionalized gold nanoparticles (DNA-AuNPs) can be synthesized using a polyvalent DNA-AuNPs conjugation approach, where AuNPs are rapidly synthesized at polyguanine strands, resulting in polyvalent DNA-AuNPs with dense DNA layers suitable for biomedical applications. Chemical synthesis techniques like vapor–liquid–solid (VLS) and various deposition methods are also commonly used for the growth of nanomaterials, including cadmium-based II–VI semiconducting materials.
What are some protocols for cdna synthesis?5 answersThere are several protocols for cDNA synthesis. One method involves mixing a lysis solution containing a chaotropic substance and a nucleic acid-binding solid-phase carrier in a sample containing RNA, adsorbing the RNA on the carrier, reverse-transcribing the RNA while keeping it adsorbed on the carrier, and eluting the synthesized cDNA with an eluent. Another protocol suggests measuring the total concentration of RNAs in the samples before treating them with RNase T1, as RNase T1 can partially digest double-stranded RNAs in the absence of single-stranded RNA. This method is used for dsRNA sequencing by nanopore sequencing.
What are the different methods used to identify lncRNAs?4 answersDifferent methods used to identify lncRNAs include traditional bio-sequencing, machine learning approaches, and deep learning-based frameworks. Traditional bio-sequencing methods involve tedious work of biological characteristic-based feature extraction procedures and are prone to artifacts during the sequencing process. Machine learning approaches, such as the Bagging method and Decision Tree algorithm in ensemble learning, have been proposed to identify lncRNAs from massive RNA sequence data. These methods have been compared with other models like Byes, Support Vector Machine, Logical Regression, Decision Tree, and Random Forest, showing higher accuracy in lncRNA identification. Deep learning-based frameworks, like lncDLSM, have been developed to differentiate lncRNAs from protein-coding transcripts without relying on prior biological knowledge. These frameworks utilize transfer learning to achieve satisfactory results in identifying lncRNAs across different species. Computational methods have also been proposed to identify misannotated lncRNAs based on sequence information alone, offering potential for characterizing the hidden proteome encoded by misannotated lncRNAs.
What are some of the most common green synthesis methods?5 answersGreen synthesis methods for nanoparticles include the use of plant extracts, microorganisms, and proteins as bio-capping and bio-reducing agents. These methods are environmentally friendly, biocompatible, and economically effective. Plant extracts contain phytoconstituents that can be used as reducing and capping agents for the synthesis of metallic and metal oxide nanoparticles. Microorganisms, such as bacteria and yeast, can also be used for the synthesis of nanoparticles, with yeast being the most promising method due to its ability to produce a large amount of nanomaterial and control the size and shape of the nanoparticles. Green synthesis methods have diverse applications in various industries, including agrochemical, food, medication delivery, cosmetics, cellular imaging, and biomedical industries. These methods offer a greener and more sustainable approach to nanomaterial synthesis, reducing the generation of hazardous byproducts and utilizing natural resources.
How to make synthesis?4 answersSynthesis can be achieved through various methods depending on the context. One method involves producing a carbonate compound from carbon monoxide and an alcohol-based compound at an anode of an electrochemical cell, followed by a dealcoholization reaction to synthesize a first product. The alcohol-based compound eliminated in the second step is recycled in the first step. Another method is high-level synthesis, where circuits are specified using conventional programming languages. This approach increases design productivity by leveraging the mathematical structure shared between functional programming languages and diagrammatic descriptions of hardware. The Geometry of Synthesis technique utilizes the game-semantic model of the programming language to reduce computational effects to signal-like message passing, supporting features such as higher-order functions, local state, concurrency, and recursion. Additionally, synthesis algorithms can be used to generate program fragments from specifications, making programs easier to write and reason about. These algorithms should support unbounded data types and guarantee correctness and applicability to software. Generalizing decision procedures into predictable and complete synthesis procedures allows for the synthesis of code that satisfies the specification, even in the presence of parametric coefficients.
What are the different types of methods employed for synthesis of nanoparticles?5 answersNanoparticles can be synthesized using various methods, including chemical, physical, and green synthesis approaches. Chemical methods involve the use of toxic chemicals and organic solvents, which can have adverse effects on health and the environment. Physical methods include techniques such as sol-gel, solvothermal process, and ion sputtering, which involve the decomposition of metals or deposition of thin layers of nanoparticles. Green synthesis, on the other hand, is an environmentally friendly approach that utilizes natural reagents such as herbs, bacteria, fungi, and agricultural waste to produce nanoparticles. This method is considered safe, cost-effective, and regenerative, with less or no toxicity to the environment. Mechanochemical processing is another method used for the solid-state synthesis of nanoparticles, where mechanical activation of chemical reactions is employed. These different methods offer a range of options for synthesizing nanoparticles with unique properties for various applications.