What are the specific industrial applications of glycolipids, lipopeptides, and viscosins synthesized by pseudomonads?5 answersGlycolipids, lipopeptides, and viscosins synthesized by pseudomonads have diverse industrial applications. Glycolipids, such as rhamnolipids, are potent antibiofilm agents with potential in various industrial sectors due to their amphiphilic structure. Lipopeptides, like pseudodesmin and viscosinamide, exhibit antimicrobial properties and can distort or disintegrate soil-borne pathogens, making them valuable for agricultural applications. Viscosins, specifically viscosin produced by Pseudomonas fluorescens SBW25, play a crucial role in biofilm dispersal, indicating their significance in biofilm formation and architecture in industrial settings. These compounds offer sustainable alternatives to synthetic surfactants, with downstream processing methods ensuring high purity levels for industrial use.
Isolation and molecular characterization of Pseudomonas species from infected freshwater fish5 answersPseudomonas species were isolated and characterized from infected freshwater fish in several studies. In Turkey, a total of 90 Pseudomonas strains were isolated from water and diseased and healthy fish, and were classified under 20 species. The strains were characterized based on various phenotypic and genotypic methods, including multilocus sequence analysis. Another study compared the identification of Pseudomonas species using MALDI-TOF MS Biotyper and 16S rDNA sequencing. Thirteen different Pseudomonas species were isolated from freshwater fish, and the identification methods showed accordance in 6 out of 15 isolates. In a study conducted in pet shops' fish tanks and home aquaria, 13 different Aeromonas species were identified, indicating the diversity of Aeromonas in freshwater fish tanks. Additionally, in a study conducted in Egypt, several Pseudomonas species, including A. hydrophila, A. veronii, A. caviae, and A. sobria, were isolated from clinically diseased fish, highlighting the presence of septicemic bacterial pathogens in freshwater fish. Furthermore, in Turkey, several putative novel Pseudomonas species were isolated from infected rainbow trouts, and their molecular characterization confirmed the presence of two novel species, Pseudomonas anatoliensis and Pseudomonas iridis.
What organic acids pseudomonas produces in carbon metabolism?5 answersPseudomonas species are known to produce a wide variety of organic acids in carbon metabolism. Gluconate, 2-ketogluconate, aketoglutarate, and pyruvate are important intermediates of glucose breakdown in Pseudomonas aeruginosa and Pseudomonas fluorescens. Additionally, Pseudomonas putida has been shown to stimulate the TCA cycle and glyoxylate shunt, resulting in higher intracellular flux via acetyl-CoA when accumulating polyhydroxyalkanoates (PHAs) from octanoic acid. This indicates that Pseudomonas species have the ability to produce organic acids such as gluconate, 2-ketogluconate, aketoglutarate, pyruvate, and acetyl-CoA as part of their carbon metabolism.
What is pseudomonas?5 answersPseudomonas is a versatile and cosmopolitan bacterial group that can occur in metal contaminated as well as clinical environments. They possess the ability to proliferate resistance to bacteria from their own or other groups. Antibiotic resistance in clinical bacteria is a growing concern, and pseudomonads have been shown to have dual resistance to metal and antibiotics through various mechanisms such as co-resistance, cross-resistance, and biofilm production. The genus Pseudomonas is a major group of rod-shaped, Gram-negative, polarly flagellated bacteria. They are ubiquitous in soil and water and can grow in simple media using a variety of organic compounds. Some strains are pathogenic for plants and animals. Pseudomonas is a diverse bacterial genus with over 60 validly described species. They are obligate aerobic chemoorganotrophs capable of living on a wide range of carbon compounds. Ps. aeruginosa, a type species of Pseudomonas, is pathogenic for humans and is often found in genitourinary, respiratory, and skin lesions.
Has Pseudomonas putida been used for production of polyketides?4 answersYes, Pseudomonas putida has been used for the production of polyketides. P. putida is a metabolically versatile bacterium that has been studied for its potential to produce various industrial products, including polyhydroxyalkanoates and terpenoids. In particular, P. putida has been used as a host microorganism for the recombinant synthesis of type III polyketide synthase (T3PKS)-derived compounds, which are widely used as nutraceuticals and medicines. The bacterium's diverse carbon metabolisms and genetic machinery make it suitable for the production of these valuable compounds. Additionally, P. putida's robust metabolism, tolerance to toxic compounds, and ease of genetic modification make it an attractive candidate for industrial applications and a good provider of the molecular machinery needed for polyketide synthesis.
Has Pseudomonas putida been used for production of indoles?0 answersPseudomonas putida has been used for the production of indigo from indole, as demonstrated by the study conducted by Choi et al.. They isolated Pseudomonas sp. BCNU 106 from industrial wastewater, which was able to produce indigo effectively when grown in the presence of p-xylene and indole. The study showed that Pseudomonas sp. BCNU 106 could be a potential candidate for industrially important indigo production. However, there is no specific mention of Pseudomonas putida being used for the production of other indoles in the abstracts provided.