What is the wavelength of UV light with maximal absorbance by purified protein?5 answersThe wavelength of UV light with maximal absorbance by purified protein varies depending on the specific protein being studied. For instance, myosin B solution shows maximum absorption at 278 nm, while a protein assay at 187 nm allows quantitation of proteins in aqueous solutions. Additionally, exposure to UV light between 290-320 nm induces aggregation in crystallin proteins. Electronic absorption spectra of proteins typically range from 185-320 nm, with some proteins exhibiting absorption beyond 350 nm. Moreover, a study comparing absorbance readings for protein determination at 600 nm using different instruments showed high correlation values. Therefore, the maximal absorbance wavelength for purified protein can vary based on the protein type and experimental conditions.
What kind of material can detected with UV 274?5 answersA material that can be detected with UV 274 is a Ga2O3/TiO2 planar heterojunction photodetector (PD) [[. This PD exhibits impressive deep-ultraviolet (DUV) sensing properties, including an ultrahigh responsivity of 275 A/W, a large specific detectivity of 6.69 x 10^16 Jones, and an external quantum efficiency of 1.35 x 10^5 under specific light intensity conditions. The PD is capable of sensing weak DUV light signals with high sensitivity and can operate in a self-powered mode. The excellent performance of this PD is attributed to the built-in electric field in the heterojunction and the dual absorption of DUV light by both the Ga2O3 and TiO2 layers. This innovative Ga2O3/TiO2 PD holds promise for energy-conserving DUV sensing systems.
What compounds could possibly have at the absorbance of 254.33 to 315 in UV-Vis?5 answersCompounds that could potentially absorb light in the range of 254.33 to 315 nm in the UV-Vis spectrum include novel UV absorbing compounds synthesized through methods involving reduction, exposure to acidic environments, and acylation. These compounds are designed for use in various applications such as sunscreen formulations, paints, plastics, fabrics, and UV protective coatings. Additionally, novel benzotriazoles have been identified as photostabilizers and solubilizers for dibenzoylmethane derivatives, potentially exhibiting absorption in the specified range. The absorption properties of these compounds make them valuable for protecting against UV radiation and enhancing the stability of related formulations.
What is the mechanism of the UV-vis absorbance of ascorbic acid?3 answersThe mechanism of the UV-vis absorbance of ascorbic acid involves several factors. Firstly, it has been observed that the absorption of UV radiation by ascorbic acid can lead to the formation of free radicals, which can then undergo further reactions. Additionally, the presence of ascorbic acid has been found to quench the fluorescence of tryptophan and protein in the eye media, thereby minimizing UV radiation to the lens. Furthermore, the presence of ascorbic acid has been shown to hinder the photodegradation of collagen molecules under UV radiation, potentially through the formation of hydrogen bonds between ascorbic acid and collagen. Finally, the absorbance of ascorbic acid itself can be affected by the presence of other substances, such as the Fe3+-Clofibric Acid complex, which has been shown to mediate the oxidation of ascorbic acid.
Chloraphenicol absorb at what wavelength in the UV spectrophotometer?5 answersChloramphenicol absorbs at a wavelength in the UV spectrophotometer, but the specific wavelength is not mentioned in the abstracts provided.
In which range in UV-Vis absorb the thiol groups?5 answersThiol groups absorb light in the UV-Vis range. The absorbance peaks for thiol groups were found to be in the range of 385-390 nm.