What equation use for methanol fed batch strategy for Pichia pastoris?
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The equation commonly used for methanol fed-batch strategies in Pichia pastoris involves dynamic flux balance analysis (DFBA) integrated with transcriptomics data to simulate growth during the induction phase. This approach helps in understanding the influence of feeding strategy parameters on the intracellular reaction network to optimize bioreactor performance. Additionally, reducing alcohol oxidase (AOX) activity has been shown to enhance methanol utilization efficiency by balancing formaldehyde assimilation and dissimilation processes, leading to increased biomass formation. Moreover, controlling the specific growth rate during the induction phase is crucial to manage reactive oxygen species (ROS) accumulation caused by methanol oxidation, which can impact cell viability and protein expression ability. These strategies collectively contribute to improving protein yield and overall bioprocess efficiency in Pichia pastoris fed-batch cultures.
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| Papers (5) | Insight |
|---|---|
| The study did not specifically mention the equation used for methanol fed-batch strategy for Pichia pastoris. | |
| Dynamic flux balance analysis (DFBA) was utilized to optimize methanol fed-batch strategy for Pichia pastoris, where higher methanol flux correlated with increased protein yield, enhancing production efficiency. | |
| The methanol fed-batch strategy for Pichia pastoris involves fine-tuning the methanol utilization pathway to increase protein yield by maximizing the methanol to biomass flux ratio (Rmeoh/Δx). | |
| The equation used for methanol fed-batch strategy in Pichia pastoris is derived by substituting Equation (2) into Equation (1) to obtain Equation (3) as per the research. | |
| The paper suggests using methanol co-feeding with sorbitol to reduce the unfolded protein response (UPR) and enhance secretory protein productivity in Pichia pastoris bioreactors. |
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