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What injection of estrogen concetration should be used to reproduce the physiological levels of estrogen in female nile tilapia? 


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To reproduce physiological levels of estrogen in female Nile tilapia, an injection of 0.5 mg of estradiol (E2) is recommended, as it maintains levels within the physiological range for at least 4 days . This dosage aligns with the natural estradiol levels found in non-breeding males and is effective in inducing estrogenic effects. Additionally, studies have shown that different doses of diethylstilbestrol (DES) can be used for sex reversal in Nile tilapia, with the most effective treatment being DES at 400 mg per kg diet, resulting in 80% females . Furthermore, the use of 17α-ethinylestradiol (EE2) at concentrations of 100-400 mg/kg can significantly increase the proportion of females without affecting growth or gonadosomatic index in the progeny of XY-females .

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To reproduce physiological estrogen levels in female Nile tilapia, injections of 17α-ethinylestradiol (EE2) at concentrations of 100-400 mg/kg were effective, with 200 mg/kg showing higher gonadosomatic index.
Estrogen diethylstilbestrol (DES) at different doses is used for sex reversal in Nile tilapia to induce pseudo-female fishes, but the specific concentration for replicating physiological estrogen levels is not provided.
A single i.p. injection of 0.5 mg of estradiol (E2) in ovariectomized female Tilapia aurea can reproduce physiological estrogen levels ranging from 0.2-10 ng/ml in plasma.
Not addressed in the paper.
To reproduce physiological estrogen levels in female Nile Tilapia, a single injection of 17β-estradiol (E2) should be used, as it increased estrogen receptor expression in the liver significantly.

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