What is RNA extraction?
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RNA extraction is a fundamental step in molecular biology experiments that involves isolating RNA molecules from cells or tissues. It is necessary to obtain high-quality and high-completeness RNA for downstream molecular biological experiments such as cDNA library construction, real-time quantitative PCR, and transcriptome sequencing. Various methods have been developed for RNA extraction, including phenol-based protocols, commercial kits, and innovative techniques. Phenol-based protocols are considered robust and can yield high quantity and quality RNA from different strains of organisms, such as Chlamydomonas . Some methods involve the use of specific solutions and techniques, such as ultrasonic breaking, centrifugation, and the addition of chloroform or isopropyl alcohol . These methods aim to avoid incomplete tissue breaking, interference of endogenous RNA enzymes, and RNA degradation during the extraction process. Overall, RNA extraction is a crucial procedure that enables the study of gene expression and molecular biology in various organisms .
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| RNA extraction is a process used to isolate RNA molecules from a biological sample. | |
05 Jul 2018 | RNA extraction is a basic procedure in molecular biology that involves isolating RNA molecules from a biological sample. |
| RNA extraction is a method described in the paper that involves preserving tissues or cells with extracting solutions, performing ultrasonic breaking, and using centrifugation to obtain RNA. | |
Patent 15 May 2018 3 Citations | RNA extraction is the process of isolating RNA from a biological sample. |
Related Questions
What is the role of phenol in RNA extraction?5 answersPhenol plays a crucial role in RNA extraction by enabling the isolation of high quantity and quality RNA from various sources. It is highlighted in multiple studies as a key component in robust RNA extraction protocols. Phenol-based methods are known for their effectiveness in obtaining RNA from challenging samples like Chlamydomonas algae, urinary sediment cells, and yeast, overcoming issues related to RNA quality and quantity. The use of phenol in extraction procedures helps in breaking down cell walls, facilitating the release of RNA molecules for downstream applications like northern blot hybridization, RT-PCR, and cDNA construction. Overall, phenol's ability to efficiently extract RNA from diverse biological samples makes it a valuable component in molecular biology research.
What is DNA extraction in biotechnology?4 answersDNA extraction in biotechnology refers to the process of isolating DNA from complex samples or environments for various applications in molecular biology. It is an essential component of many molecular biology methods, including disease diagnosis, food microbiology, and environmental microbiology. There are several DNA extraction methods available, including commercial kits that utilize different technologies for DNA purification. These methods can be automated, allowing for high-throughput analysis of samples. DNA extraction is often combined with polymerase chain reaction (PCR) to amplify specific DNA sequences for further analysis. The quality of the extracted DNA is crucial for accurate detection methods, such as hybridization, amplification, and sequencing. New technologies, such as next-generation sequencing, are rapidly evolving and replacing existing detection methods. Additionally, there are inventions involving compositions for lysing cells and stabilizing RNA in whole blood, expanding the range of products for DNA extraction. A portable device has also been developed for extracting DNA from tissue samples, providing a convenient and efficient method.
How to extract rna from blood sample using trizol method step by step?4 answersTo extract RNA from a blood sample using the Trizol method, the following steps can be followed. First, collect the blood using a negative pressure blood sampling tube filled with an anticoagulant and Trizol agent. Store the blood at room temperature for 30 days. Next, add Trizol reagent to the pretreated sample in an RNase-free centrifuge tube and mix. Extract chloroform and add isopropanol to obtain a white sediment. Wash and centrifuge the sediment, then discard the supernatant. Finally, add diethyl pyrocarbonate to dissolve the sediment and obtain a purified RNA sample. Another method involves using an integrated centrifugo-pneumatic micro-homogenizer, which efficiently lyses whole blood and yields the aqueous phase with the total RNA content. These methods provide high-quality RNA extraction from blood samples.
What is the best alternatives for RNA extraction?2 answersبهترین جایگزین برای استخراج RNA عبارتند از استفاده از دیتیوتریتول (DTT) به جای β-مرکاپتواتانول (β-ME) به عنوان عامل کاهنده. جایگزین دیگر سازگاری روش صفحه Qiagen RNeasy 96 برای استخراج RNA از سواب های نازوفارنگ است که معتبر شده و نشان داده شده است که معادل روش Roche-Magnapureاست. علاوه بر این، لیز مستقیم سلول با یک معرف مبتنی بر فنل گوانیدین یا یک عامل تفکیک مبتنی بر پروتئاز بدون منبع حیوانی می تواند برای جلوگیری از تجزیه RNA در طول تریپسینیزاسیوناستفاده شود. یکی دیگر از روش های کارآمد و ساده برای استخراج RNA استفاده از دانه های مغناطیسی پوشش داده شده با سیلیکا است که با اکسید گرافن (GO) به عنوان جاذباست. این جایگزین ها به اطمینان از استخراج RNA با کیفیت بالا و حفظ یکپارچگی RNA برای کاربردهای پایین دستیکمک می کنند.
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