What is the best method to check if two proteis that co-localize directly interact??
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The most effective method to determine if two proteins that co-localize directly interact is through techniques like Proximity-Dependent PAINT (PD-PAINT) and COLA. PD-PAINT allows sub-diffraction imaging of protein pairs by decoupling proximity detection from optical resolution, enabling nanoscale resolution imaging. On the other hand, COLA is a proteomics-based strategy that rapidly detects protein-protein co-localizations on a global scale, indicating functional interactions by matching proteins with similar subcellular localization signatures . These methods leverage the spatial colocalizations between proteins to infer their direct interactions, providing valuable insights into the dynamic nature of protein interaction networks within cells.
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| Papers (5) | Insight |
|---|---|
| COLA (Colocalisation Analysis) is a proteomics strategy that detects protein-protein co-localisations, indicating potential functional interactions based on subcellular localisation similarity. | |
| The Locational and Topological Overlap Model (LTOM) is the best method to check if two proteins that co-localize directly interact by considering protein localization and topological overlap in networks. | |
28 Oct 2015 1 Citations | Not addressed in the paper. |
| The best method to check if co-localizing proteins directly interact is Proximity-Dependent PAINT (PD-PAINT), which decouples proximity detection from optical resolution, providing nanoscale imaging of protein pairs. | |
| The best method to check if two proteins that co-localize directly interact is through spatial colocalization analysis (COLA) using mass spectrometry to reveal functional interactions based on significant colocalizations. |
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