What is the rate of recessive mutations introduced by crispr-cas9?
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The rate of recessive mutations introduced by CRISPR-Cas9 varies depending on the system and target. Studies have shown that CRISPR-Cas9 can induce off-target mutations, which may include recessive mutations . However, the specificity of CRISPR-Cas9 for genome editing has been observed to be high in certain systems, such as grapevine, with minimal off-target effects. Strategies like using a heat-shock inducible CRISPR/Cas9 system have been developed to control genome editing activities, limiting off-target effects and improving precision. Additionally, the analysis of CRISPR-Cas9-induced substitutions in high-throughput sequencing data has revealed that these substitutions are generated by the non-homologous end joining (NHEJ) repair pathway, which can lead to the introduction of mutations, including recessive ones.
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| Papers (4) | Insight |
|---|---|
| The heat-shock inducible CRISPR/Cas9 system in rice showed a low rate of targeted mutations before heat shock, but a higher rate after treatment, leading to heritable monoallelic and biallelic mutations. | |
13 Citations | The rate of apparent substitution mutations induced by CRISPR-Cas9 in human cells was found to be 0.8% on average, with no specific nucleotide preference patterns observed. |
| CRISPR-Cas9 introduces up to 1700 off-target mutations due to loss of heterozygosity, potentially affecting recessive alleles, with a 99-fold lower efficiency in heterozygous yeast genomes. | |
42 Citations | CRISPR/Cas9 introduced recessive mutations in 5% of mice via homologous recombination and 29% at an off-target site with three mismatches, highlighting potential off-target concerns in genome editing. |
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