What is use the of metabolic engineering? limonene?
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Metabolic engineering is a technique used to manipulate the metabolic pathways of organisms in order to increase the production of valuable metabolites. It involves altering enzyme kinetics, regulatory proteins, and other factors to optimize the production of desired compounds. Limonene, a monoterpene compound, has various applications in industries such as food, cosmetics, and pharmaceuticals. Metabolic engineering has been used to enhance the production of limonene in different organisms. For example, strains of Ashbya gossypii, Saccharomyces cerevisiae, Yarrowia lipolytica, and Escherichia coli have been engineered to produce limonene at high titers . These studies demonstrate the potential of metabolic engineering in increasing the yield of limonene and other valuable compounds.
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328 Citations | Metabolic engineering is used to produce limonene, a valuable monoterpene used in the production of commodity chemicals and medicinal compounds. |
| Metabolic engineering is used to increase limonene production in Yarrowia lipolytica, a yeast, for applications in flavoring, pharmaceutical, and biodiesel products. | |
| Metabolic engineering is used to efficiently synthesize limonene, a compound widely used in food additives, pharmaceutical products, fragrances, and toiletries. | |
| Metabolic engineering is used to produce limonene from alternative carbon sources, such as xylose, using engineered strains of Ashbya gossypii. |
Related Questions
Are there studies on allergic respiratory sensitization of limonene?4 answersStudies have investigated the impact of limonene on allergic respiratory sensitization. Research has shown that limonene, a terpene found in citrus fruits, can reduce inflammation and airway reactivity in asthma models by binding to A2A and A2B receptors. Inhalation of limonene and its reaction products did not exacerbate allergic lung inflammation; in fact, they reduced allergic inflammation possibly due to antioxidant properties. Limonene hydroperoxides have been identified as common causes of contact allergy, with positive patch test reactions observed in patients, supporting their inclusion in allergy screenings. Furthermore, limonene has been found to restore A2A receptor-mediated aortic relaxation in asthmatic mice, indicating its potential in alleviating allergic respiratory sensitization.
What is the limonene compound?5 answersLimonene is a monocyclic monoterpene compound found abundantly in citrus plants like lemon, orange, and grape. It is a transparent, colorless chemical with D and L isomers, commonly present in citrus fruit peels and essential oils. Limonene has various applications, including as a flavor and fragrance additive in perfumes, soaps, foods, and beverages due to its high-quality fragrance properties. Additionally, limonene has low toxicity and is used in the production of commodity chemicals, biodiesel products, medicinal compounds, and pharmaceuticals. Furthermore, limonene has been shown to exhibit antifungal properties, inhibiting the growth of fungi like Fusarium graminearum and reducing mycotoxin production. Its presence in Eucalyptus leaves has been linked to rust resistance in plants, with high limonene levels inhibiting fungal growth and spore germination.
What is the effect of limonene on tick behavior?5 answersLimonene has been found to have various effects on different behaviors and physiological processes, but there is no specific mention of its effect on tick behavior in the provided abstracts. Limonene has been shown to have anti-stress effects and can inhibit hypothalamic-pituitary-adrenal (HPA) activity. It also has antidepressant-like effects mediated through inhibition of neuroinflammation and attenuation of nitrite levels in the hippocampus. Limonene can influence the crystallization behavior of lipid systems, such as reducing solid fat content and consistency, and accelerating polymorphic transition. In the context of chocolate, the use of limonene can potentially result in increased bloom formation due to its effect on cocoa butter crystallization and polymorphism transformation rate. However, there is no direct information about the effect of limonene on tick behavior in the provided abstracts.
What are Implication of metabolic engineering and synthetic biology in plant natural products biosynthesis?5 answersMetabolic engineering and synthetic biology have significant implications in plant natural product biosynthesis. These approaches address major challenges in the field, such as the need for rapid testing of enzyme candidates and the lack of complex substrates. By linking metabolic engineering with gene discovery, researchers can expedite the elucidation process and create a platform for sustainable production of plant metabolites. Synthetic biology and metabolic engineering have also enabled the tailor-made production of aromatic natural products using engineered microbial cell factories, overcoming the low content and extraction difficulties associated with these compounds. Additionally, plant synthetic biology combines engineering principles with plant biology, expanding the host range from single-celled microbes to complex plant systems. This emerging field holds great promise for future agriculture and industry, providing opportunities for the production of plant natural products on a large scale.
Can you give me the metabolic engeneering strategies using medical plant in pharmaceutical?5 answersMetabolic engineering strategies in medicinal plants for pharmaceutical purposes include increasing the flux of precursors, blocking competitive pathways, introducing new metabolic pathways, overcoming rate-limiting steps, and overexpressing regulatory genes or transcription factors. These strategies aim to enhance the production of bioactive compounds such as polyphenols, alkaloids, terpenes, and saponins. Genetic modification technologies, including the CRISPR/Cas9 tool, have been utilized to manipulate genes involved in the biosynthetic pathways of secondary metabolites. CRISPR/Cas9 enables targeted mutagenesis, gene knockouts, and regulation of gene transcription, leading to the production of specific secondary metabolites. Metabolic engineering approaches have also been applied in microbial systems, such as bacteria, to optimize the production of valuable metabolites, including pharmaceuticals. These strategies offer promising possibilities for overcoming the limitations in obtaining desired plant compounds and enhancing the production of bioactive compounds in medicinal plants for pharmaceutical applications.
What are the interesting papers in metabolic engineering?5 answersMetabolic engineering has seen significant advancements in the past few decades. Some interesting papers in this field include the following:
- Liu and Wang analyze the development of metabolic engineering over the past 30 years and summarize the novel theories, techniques, strategies, and applications that have emerged in this field.
- Antoniewicz discusses the principles of metabolic flux analysis and its applications in metabolic engineering practice.
- Mazzoli and Liu highlight the diverse applications of metabolic engineering, including medical, environmental, and biorefinery fields.
- Tang et al. propose a hybrid algorithm to optimize the production rate of succinate and lactate, showcasing the potential of using computational procedures in metabolic engineering.
- Yarmush and Banta review the accomplishments made in the medical arena using metabolic modeling and pathway manipulation, with a focus on disease-state physiology and therapeutic applications.
These papers provide valuable insights into the development, techniques, and applications of metabolic engineering, making them interesting contributions to the field.