What ist the most sensitive method to determine katecholamines from blood?
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The most sensitive method to determine ketamine in blood is a fluorescence genosensor based on DNA-templated silver nanoclusters (DNA-AgNCs) probes . This genosensor allows for the determination of ketamine in the concentration range of 0.0001–20 μg/mL with a detection limit of 0.06 ng/mL . Another method for quantifying ketamine in blood is a magnetic solid-phase microextraction (MSPE) procedure based on multi-walled carbon nanotubes (MWNTs) combined with gas chromatography-mass spectrometry (GC-MS) . This method has a linear range of 0.2–50 ng/mL with limits of detection below the cut-off values recommended by the United Nations Office on Drugs and Crime (UNODC) . Additionally, a biotin–avidin amplified enzyme-linked immunosorbent assay (BA-ELISA) can be used to determine ketamine in biological samples . This method has a linear range of 0.1–1000 µg/L with a detection limit of 0.03 µg/L .
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