What the cell for propagation canine Parvo virus?4 answersThe cell lines used for the propagation of canine parvovirus (CPV) are Crandell Rees Feline Kidney (CRFK) and Madin Darby Canine Kidney (MDCK). F81 cells are also used as host cells for CPV proliferation. Additionally, a cell overexpressing single strand binding protein compared to a wild-type cell is used for the production of parvovirus vector particles. CPV isolates have been propagated in CRFK cell lines for virus isolation and characterization.
What the cell for propagation Canine parainfluenza virus?4 answersThe Vero cell line was used for propagation of Canine parainfluenza virus (CPIV).
What is CART cell?5 answersChimeric antigen receptor T (CART) cells are a form of adoptive T-cell immunotherapy that have shown significant therapeutic efficacy in the treatment of cancer. CART cells are genetically engineered T lymphocytes that express chimeric antigen receptors (CARs), which combine components of T cells and antibodies to enhance their anti-tumor effect. These CARs allow CART cells to recognize and target specific cancer cells. CART cell therapy has gained FDA approval for certain cancers and is being investigated in numerous clinical trials for additional designs and targets. However, response rates to CART cell therapy vary between studies, patients, and cancers, highlighting the need for computational frameworks to predict CART cell function and clinical efficacy. Various imaging platforms are also being developed to track CART cells in vivo, providing valuable information for treatment optimization.
What is asexual propagation?3 answersAsexual propagation refers to the method of plant reproduction that does not involve the use of seeds. It is a technique used to create new plants that are genetically identical to the parent plant. Asexual propagation can be achieved through various methods such as stem cuttings, root suckers, or tuber division. This technique is commonly used in fruit propagation, where it is necessary to obtain uniform and high-quality plants for orchard cultivation. It has also been employed in the propagation of native plant species in prairies, overcoming challenges such as low seed production and complex seed dormancy mechanisms. Additionally, asexual propagation methods have been developed for specific plant species such as Baccharis dracunculifolia and zingiber striolatum diels, where stem cuttings and tuber division have been found to be effective. Asexual propagation offers advantages such as faster propagation, uniformity in plant characteristics, and overcoming issues associated with seed availability and dormancy.
What cells can be used to generate ipsc?5 answersMultiple cell types can be used to generate induced pluripotent stem cells (iPSCs). These include peripheral blood mononuclear cells (PBMCs), mesenchymal stromal cells (MSCs), fibroblasts, keratinocytes, and urine cells. PBMCs have been reprogrammed into iPSCs using non-integrating Sendai viruses, and PBMC-iPSCs have demonstrated high cytogenetic stability and minimal genomic aberrations. MSCs have also been used as a reprogramming source for iPSC induction. Fibroblasts have been commonly used as a starting cell source for iPSC generation. Keratinocytes and urine cells have also shown promise as easily accessible somatic cell sources for iPSC generation. These various cell types offer different advantages and can be used to generate iPSCs for different research and therapeutic applications.
What are type of cells used in tissue engineering?4 answersTissue engineering utilizes various types of cells for the fabrication of artificial tissue and organs. Stem cells are a prominent cell source in tissue engineering, offering the potential for regeneration and replacement of damaged tissues. Resident human cardiac stem cells have been discovered, challenging the previous belief that the human heart lacks intrinsic regenerative potential. Autologous endothelial (precursor) cells are being investigated for seeding biological or artificial scaffolds in tissue engineering of heart valves and small calibre vessels. Additionally, the use of expandable cells, such as stem cells, is of interest in regenerative medicine. The specific types of cells used in tissue engineering depend on the type of tissue being engineered and may include stem cells, resident cardiac stem cells, and autologous endothelial cells.